約 4,071,594 件
https://w.atwiki.jp/elvis/pages/4211.html
Corporate and Municipal Securities Richard D Irwin? R. Kuhn? RichardDIrwin? R.Kuhn? Substores-UnknownASINs-35 Subjects-Business&Investing-Economics-PublicFinance? Subjects-Business&Investing-Finance? Subjects-Nonfiction-Economics-PublicFinance? Subjects-Professional&Technical-Accounting&Finance-Economics-PublicFinance? Mergers Acquisitions and Leveraged Buyouts Irwin Professional Pub? Robert Lawrence Kuhn? IrwinProfessionalPub? RobertLawrenceKuhn? Substores-UnknownASINs-35 Subjects-Business&Investing-Finance? Subjects-Business&Investing-Management&Leadership-Production&Operations? Subjects-Professional&Technical-BusinessManagement-Management&Leadership-Production&Operations? Mortgage and Asset Securitization Irwin Professional Pub? Rick Kuhn? IrwinProfessionalPub? RickKuhn? Substores-UnknownASINs-35 Subjects-Business&Investing-Economics-PublicFinance? Subjects-Business&Investing-Finance? Subjects-Business&Investing-General? Subjects-Business&Investing-PersonalFinance-General? Subjects-Nonfiction-Economics-PublicFinance? Subjects-Professional&Technical-Accounting&Finance-Economics-PublicFinance? The Spreadsheet Style Manual Irwin Professional Pub? David Harrison? John W. Yu? IrwinProfessionalPub? DavidHarrison? JohnW.Yu? Substores-UnknownASINs-35 Subjects-Business&Investing-General? Subjects-Business&Investing-Management&Leadership? Subjects-Professional&Technical-BusinessManagement-Management&Leadership? Subjects-Computers&Internet-Software-General? The Data Exchange Moving Data Between 1-2-3 dBASE and Other Popular Programs Irwin Professional Pub? Alfred Poor? IrwinProfessionalPub? AlfredPoor? Substores-UnknownASINs-35 Subjects-Computers&Internet-Programming-General? Subjects-Computers&Internet-Databases-SpecificDatabases-dBASE? Subjects-Computers&Internet-Software-General? Index to the Library of Investment Banking Richard D Irwin? Rick Kuhn? RichardDIrwin? RickKuhn? Substores-UnknownASINs-35 Subjects-Business&Investing-Finance? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-General? Classics an Investors Anthology Richard D Irwin? James R. Vertin? Charles D. Ellis? RichardDIrwin? JamesR.Vertin? CharlesD.Ellis? Substores-UnknownASINs-35 Subjects-Business&Investing-Finance? Subjects-Business&Investing-Investing-Introduction? Classics II Another Investor's Anthology Irwin Professional Pub? James R. Vertin? Charles D. Ellis? IrwinProfessionalPub? JamesR.Vertin? CharlesD.Ellis? Substores-UnknownASINs-35 Subjects-Business&Investing-Economics-PublicFinance? Subjects-Business&Investing-General? Subjects-Business&Investing-Investing-General? Subjects-Business&Investing-Investing-Introduction? Subjects-Nonfiction-Economics-PublicFinance? Subjects-Professional&Technical-Accounting&Finance-Economics-PublicFinance? Cash Flow and Security Analysis Irwin Professional Pub? Kenneth S. Hackel? Joshua Livnat? IrwinProfessionalPub? KennethS.Hackel? JoshuaLivnat? Substores-UnknownASINs-35 Subjects-Business&Investing-Finance-Budgeting? Subjects-Business&Investing-Finance-CorporateFinance? Subjects-Business&Investing-General? Subjects-Business&Investing-Investing-Stocks? Subjects-Professional&Technical-Accounting&Finance-Finance-Budgeting? Subjects-Professional&Technical-Accounting&Finance-Finance-CorporateFinance? Hospital Home Care Strategic Management for Integrated Care Delivery Amer Hospital Pub? Dan Lerman? Eric B. Linne? AmerHospitalPub? DanLerman? EricB.Linne? Substores-UnknownASINs-35 Subjects-Health,Mind&Body-General? Subjects-Medicine-Administration&Policy-HospitalAdministration? Subjects-Medicine-Nursing-Home&CommunityCare? Subjects-Medicine-Nursing-NursingHomeCare? Subjects-Nonfiction-CurrentEvents-Poverty-SocialServices&Welfare? Subjects-Professional&Technical-Medical-Administration&MedicineEconomics-HospitalAdministration? Subjects-Professional&Technical-Medical-Nursing-Home&CommunityHealth? Subjects-Professional&Technical-Medical-Nursing-NursingHomeCare? Subjects-Professional&Technical-Medical-Nursing-Long-TermCare? Subjects-Science-General Subjects-Science-Medicine-Administration&Policy-HospitalAdministration? Subjects-Science-Medicine-Nursing-Home&CommunityCare? Subjects-Science-Medicine-Nursing-NursingHomeCare? Time and Money Irwin Professional Pub? Robert M. Crowe? IrwinProfessionalPub? RobertM.Crowe? undefined undefined Self Directed Work Teams Universal (F72ax) Irwin Professional Pub? Jack D. Orsburn? IrwinProfessionalPub? JackD.Orsburn? Substores-UnknownASINs-35 Subjects-Business&Investing-Industries&Professions-HumanResources&PersonnelManagement? Subjects-Professional&Technical-BusinessManagement-HumanResources&PersonnelManagement? The Business Inv Alm '92 McGraw-Hill Education? Levine? McGraw-HillEducation? Levine? Substores-UnknownASINs-35 Subjects-Business&Investing-Economics-EconomicConditions? Subjects-Business&Investing-International-EconomicConditions? Subjects-Nonfiction-Economics-EconomicConditions? Subjects-Professional&Technical-Accounting&Finance-Economics-EconomicConditions? Reshaping the Equity Markets A Guide for the 1990s Richard D Irwin? Robert A. Schwartz? RichardDIrwin? RobertA.Schwartz? Substores-UnknownASINs-35 Subjects-Business&Investing-Economics-PublicFinance? Subjects-Business&Investing-Investing-General? Subjects-Nonfiction-Economics-PublicFinance? Subjects-Professional&Technical-Accounting&Finance-Economics-PublicFinance? Masterg Art Q a-Pb Longman Higher Education? MARTEL? LongmanHigherEducation? MARTEL? Substores-UnknownASINs-35 Subjects-Business&Investing-General? Firing on All Cylinders The Service/Quality System for High-Powered Corporate Performance Business One Irwin? Jim Clemmer? Barry Sheehy? BusinessOneIrwin? JimClemmer? BarrySheehy? Substores-UnknownASINs-35 Subjects-Business&Investing-Management&Leadership-Management? Subjects-Business&Investing-Marketing&Sales? Subjects-Business&Investing-Reference-BusinessStudies? Subjects-Professional&Technical-BusinessManagement-Management&Leadership-Management? Subjects-Professional&Technical-BusinessManagement-Marketing&Sales? Investment Policy How to Win the Loser's Game Irwin Professional Pub? Charles D. Ellis? IrwinProfessionalPub? CharlesD.Ellis? undefined undefined The Handbook of Employee Benefits Design, Funding and Administration Irwin Professional Pub? Jerry S. Rosenbloom? IrwinProfessionalPub? JerryS.Rosenbloom? Substores-UnknownASINs-35 Subjects-Business&Investing-General? Subjects-Business&Investing-Industries&Professions-HumanResources&PersonnelManagement? Subjects-Professional&Technical-BusinessManagement-HumanResources&PersonnelManagement? Fables from Aesop One to Six Wright Group/ Mcgraw-Hill? Val Biro? WrightGroup/Mcgraw-Hill? ValBiro? Substores-UnknownASINs-35 Subjects-Literature&Fiction-WorldLiterature-Mythology-General? Tales of South Asia (Including Teachers Notes) Wright Group/ Mcgraw-Hill? Beulah Candappa? Derek Collard? WrightGroup/Mcgraw-Hill? BeulahCandappa? DerekCollard? undefined undefined Just This Once Wright Group/ Mcgraw-Hill? Cowley? WrightGroup/Mcgraw-Hill? Cowley? Substores-UnknownASINs-35 Subjects-Children sBooks-Ages4-8-General? Joe's Gang Wright Group/ Mcgraw-Hill? Martin Waddell? WrightGroup/Mcgraw-Hill? MartinWaddell? undefined undefined The Penguin/Big Book (First Nature Watch) Wright Group/ Mcgraw-Hill? WrightGroup/Mcgraw-Hill? undefined undefined The Polar Bear (First Nature Watch) Wright Group/ Mcgraw-Hill? WrightGroup/Mcgraw-Hill? undefined undefined Start With Rhymes 7-12 Wright Group/ Mcgraw-Hill? Aidan Warlow? WrightGroup/Mcgraw-Hill? AidanWarlow? Substores-UnknownASINs-35 Subjects-Children sBooks-Baby-3-NurseryRhymes-General? Buzzing Flies Wright Group/ Mcgraw-Hill? Joy Cowley? WrightGroup/Mcgraw-Hill? JoyCowley? Substores-UnknownASINs-35 Subjects-Children sBooks-Baby-3? Dragon With a Cold Wright Group/ Mcgraw-Hill? Cowley? WrightGroup/Mcgraw-Hill? Cowley? undefined undefined The New Man Wright Group/ Mcgraw-Hill? John Tully? WrightGroup/Mcgraw-Hill? JohnTully? Substores-UnknownASINs-35 Subjects-Literature&Fiction? Scat! Scat! Wright Group/ Mcgraw-Hill? Val Marshall? Bronwyn Tester? Susie Danlen? WrightGroup/Mcgraw-Hill? ValMarshall? BronwynTester? SusieDanlen? Substores-UnknownASINs-35 Subjects-Children sBooks-Baby-3? Bakers Bluejay Yarn Redpath Pr? Mark Twain? RedpathPr? MarkTwain? undefined undefined Taste (Perfect Presents Story-Gifts Ser) Redpath Pr? Roald Dahl? RedpathPr? RoaldDahl? Substores-UnknownASINs-35 Subjects-Literature&Fiction-General-Contemporary? A P Redpath Pr? John Updike? RedpathPr? JohnUpdike? undefined undefined Diary of a New Born Father (Perfect Presents Story-Gifts) Redpath Pr? Bob Greene? RedpathPr? BobGreene? undefined undefined Hour of Lead (Perfect Presents Story-Gifts Series) Redpath Pr? Anne Morrow Lindbergh? Etienne Delessert? RedpathPr? AnneMorrowLindbergh? EtienneDelessert? Substores-UnknownASINs-35 Subjects-Literature&Fiction-WorldLiterature-UnitedStates? Revolt of Mother Redpath Pr? Mary Eleanor Wilkins Freeman? RedpathPr? MaryEleanorWilkinsFreeman? undefined undefined Roses, Rhododendron Redpath Pr? Alice Adams? RedpathPr? AliceAdams? undefined undefined Don the True Story of a Young Person Redpath Pr? Garrison Keillor? RedpathPr? GarrisonKeillor? undefined undefined The Three Fat Women of Antibes (Perfect Presents Story-Gifts Series) Redpath Pr? W. Maughan? RedpathPr? W.Maughan? Substores-UnknownASINs-35 Subjects-Literature&Fiction-General-Contemporary? The Gift of the Magi Love at All Costs Redpath Pr? O. Henry? RedpathPr? O.Henry? undefined undefined Who Am I This Time For Romeos and Juliets Redpath Pr? Kurt Vonnegut? RedpathPr? KurtVonnegut? Substores-UnknownASINs-35 Subjects-Literature&Fiction-Authors,A-Z-(V)-VonnegutJr.,Kurt? Subjects-Literature&Fiction-General-Literary? Chronicle Vocational School Manual 2003-2004 A Directory of Accredited, Approved, Certified, Licensed or Registered Vocational and Technical Schools (Chronicle Vocational School Manual) Chronicle Guidance Pubns Inc? ChronicleGuidancePubnsInc? Substores-UnknownASINs-35 Subjects-Business&Investing-Careers-VocationalGuidance? Subjects-Nonfiction-Education-Counseling-VocationalGuidance? Subjects-Nonfiction-Education-General? Subjects-Professional&Technical-Education-General? Subjects-Professional&Technical-Education-Counseling-VocationalGuidance? Subjects-Reference-Careers-VocationalGuidance? Chronicle Financial Aid Guide 2003-2004 Scholarships and Loans for High School Students, College Undergraduates, Graduates, and Adult Learners (Chronicle Financial Aid Guide) Chronicle Guidance Pubns Inc? ChronicleGuidancePubnsInc? Substores-UnknownASINs-35 Subjects-Nonfiction-Education-College&University-FinancialAid? Subjects-Reference-Education-FinancialAid? Subjects-Reference-General? GURPS Steve Jackson Games,U.S.? SteveJacksonGames,U.S.? Substores-UnknownASINs-35 Subjects-Entertainment-Puzzles&Games-RolePlaying&Fantasy? GURPS Steve Jackson Games,U.S.? SteveJacksonGames,U.S.? Substores-UnknownASINs-35 Subjects-Entertainment-Puzzles&Games-RolePlaying&Fantasy? GURPS Steve Jackson Games,U.S.? SteveJacksonGames,U.S.? Substores-UnknownASINs-35 Subjects-Entertainment-Puzzles&Games-RolePlaying&Fantasy? Gurps Vikings Wizard s Attic,US? Steve Jackson? Wizard sAttic,US? SteveJackson? ユーズドブック(洋書)-Comics&GraphicNovels? Substores-UnknownASINs-35 Subjects-Comics&GraphicNovels-GraphicNovels-General? Gurps Vehicles From Chariots to Cybertanks...and Beyond! Steve Jackson Games? David L. Pulver? SteveJacksonGames? DavidL.Pulver? ユーズドブック(洋書)-ScienceFiction&Fantasy? Substores-UnknownASINs-35 Subjects-Entertainment-Puzzles&Games-General? Subjects-Entertainment-Puzzles&Games-RolePlaying&Fantasy-General? Subjects-ScienceFiction&Fantasy-Gaming-General? Yamara Steve Jackson Games,U.S.? SteveJacksonGames,U.S.? Substores-UnknownASINs-35 Subjects-Entertainment-Puzzles&Games-RolePlaying&Fantasy? Voodoo Steve Jackson Games? C. J. Carella? SteveJacksonGames? C.J.Carella? Substores-UnknownASINs-35 Subjects-Entertainment-Puzzles&Games-RolePlaying&Fantasy? GURPS Steve Jackson Games,U.S.? David L. Pulver? SteveJacksonGames,U.S.? DavidL.Pulver? Substores-UnknownASINs-35 Subjects-Entertainment-Puzzles&Games-RolePlaying&Fantasy? Substores-UnknownASINs-35 洋書
https://w.atwiki.jp/elvis/pages/7989.html
California Commercial Code 2003 West Group? West? WestGroup? West? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-General? Subjects-Nonfiction-Law-General? Subjects-Professional&Technical-Law-General? Business Statistics Contemporary Decision Making West Group? Ken Black? WestGroup? KenBlack? ジャンル別? Substores-UnknownASINs-8 Subjects-Science-General West's Federal Taxation Individual Income Taxes 1997 (Annual) West Group? William H., Jr Hoffman? WestGroup? WilliamH.,JrHoffman? ジャンル別? Substores-UnknownASINs-8 Subjects-Business&Investing-Industries&Professions-Accounting-Taxes-TaxLaw? Subjects-Law-Business-Taxation? Subjects-Law-General? Subjects-Nonfiction-Law-Business-Taxation? Subjects-Nonfiction-Law-General? Subjects-Professional&Technical-Law-General? Subjects-Professional&Technical-Accounting&Finance-Industries&Professions-Accounting-Taxes-TaxLaw? Subjects-Reference-General? Lawyering Skills Mediation and Arbitration (American Casebooks) West Group? Peter B. Knapp? Ann Juergens? Roger S. Haydock? WestGroup? PeterB.Knapp? AnnJuergens? RogerS.Haydock? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-Business-Arbitration,Negotiation&Mediation? Subjects-Law-One-L-CivilProcedure? Subjects-Nonfiction-Law-Business-Arbitration,Negotiation&Mediation? Subjects-Nonfiction-Law-One-L-CivilProcedure? Invitation to Oceanography Jones & Bartlett Pub? Paul R. Pinet? Jones&BartlettPub? PaulR.Pinet? ジャンル別? Substores-UnknownASINs-8 Subjects-Science-General Subjects-Science-Nature&Ecology-Oceans&Seas-Oceanography-General? Invitation to Oceanography (Earth Science Series) West Group? Paul R. Pinet? WestGroup? PaulR.Pinet? ジャンル別? Substores-UnknownASINs-8 Subjects-Science-General Subjects-Science-Nature&Ecology-Oceans&Seas-Oceanography-General? Human Intimacy Wadsworth Pub Co? Frank D. Cox? WadsworthPubCo? FrankD.Cox? ジャンル別? Substores-UnknownASINs-8 Subjects-Health,Mind&Body-MentalHealth-General? Prosser and Keeton on the Law of Torts West Group? Dan B. Dobbs? Robert E. Keeton? David G. Owen? W. Page Keeton? WestGroup? DanB.Dobbs? RobertE.Keeton? DavidG.Owen? W.PageKeeton? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-Business-Torts? Subjects-Law-Series-Hornbooks? Subjects-Nonfiction-Law-Business-Torts? Subjects-Nonfiction-Law-Series-Hornbooks? Subjects-Professional&Technical-Law-Business-Torts? Map Workbook to Accompany World Civilizations Wadsworth Pub Co? Philip J. Adler? Arthur Durand? WadsworthPubCo? PhilipJ.Adler? ArthurDurand? ジャンル別? Substores-UnknownASINs-8 Subjects-Reference-StudyGuides? Primary Source Document Workbook for World Civilizations Wadsworth Pub Co? Philip J. Adler? WadsworthPubCo? PhilipJ.Adler? ジャンル別? Substores-UnknownASINs-8 Student Study Guide to Accompany World Civilizations Wadsworth Pub Co? Philip J. Adler? Raymond Hylton? WadsworthPubCo? PhilipJ.Adler? RaymondHylton? ジャンル別? Substores-UnknownASINs-8 Subjects-Reference-StudyGuides? Geolink West Electronic Resource Library for Geo. Brooks/Cole Pub Co? Paul Pinet? Reed Wicander? Brooks/ColePubCo? PaulPinet? ReedWicander? ジャンル別? Substores-UnknownASINs-8 Subjects-Science-General Lawyering Skills Interviewing, Counseling and Negotiation West Group? Peter B. Knapp? Ann Juergens? Roger S. Haydock? WestGroup? PeterB.Knapp? AnnJuergens? RogerS.Haydock? ジャンル別? Substores-UnknownASINs-8 Subjects-Law? Subjects-Nonfiction-Law? Lawyering Skills Depositions and Trial West Group? Peter B. Knapp? Ann Juergens? Roger S. Haydock? WestGroup? PeterB.Knapp? AnnJuergens? RogerS.Haydock? ジャンル別? Substores-UnknownASINs-8 Subjects-Law? Subjects-Nonfiction-Law? Lawyering Skills Mediation and Arbitration West Group? Peter B. Knapp? Ann Juergens? Roger S. Haydock? WestGroup? PeterB.Knapp? AnnJuergens? RogerS.Haydock? ジャンル別? Substores-UnknownASINs-8 Subjects-Law? Subjects-Nonfiction-Law? Lawyering Skills Interviewing, Counseling and Negotiations; Mediation and Arbitration; Depositions and Trial West Group? Peter B. Knapp? Ann Juergens? Roger S. Haydock? WestGroup? PeterB.Knapp? AnnJuergens? RogerS.Haydock? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-One-L-SurvivalGuides? Subjects-Nonfiction-Law-One-L-SurvivalGuides? Federal Civil Judicial Procedure and Rule 1996 West Group? WestGroup? ジャンル別? Substores-UnknownASINs-8 Subjects-Law? Subjects-Nonfiction-Law? Intermediate Algebra A Graphing Approach Thomson Learning? Dennis C. Ebersole? ThomsonLearning? DennisC.Ebersole? ジャンル別? Substores-UnknownASINs-8 Subjects-Science-General Foundations of Personal Fitness Glencoe/Mcgraw-Hill? McGraw-Hill? Glencoe/Mcgraw-Hill? McGraw-Hill? ジャンル別? Substores-UnknownASINs-8 Subjects-Health,Mind&Body-Exercise&Fitness-General? Subjects-Nonfiction-Education-Pedagogy? Subjects-Professional&Technical-Education-Pedagogy? Family Law Delmar Pub? William P. Statsky? DelmarPub? WilliamP.Statsky? ジャンル別? Substores-UnknownASINs-8 Subjects-Law? Subjects-Nonfiction-Law? Introduction to Traffic Investigation and Enforcement West Group? Aric Steven Frazier? WestGroup? AricStevenFrazier? ジャンル別? Substores-UnknownASINs-8 Subjects-Law? Subjects-Nonfiction-CurrentEvents-Poverty-SocialServices&Welfare? Subjects-Nonfiction-Law? Introduction to Nutrition Thomson Learning? Terry S. Maness? ThomsonLearning? TerryS.Maness? ジャンル別? Substores-UnknownASINs-8 Subjects-Science-General Problem Supplement to Cases and Materials on Torts (American Casebooks) West Group? Grady? WestGroup? Grady? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-Business-Torts? Subjects-Nonfiction-Law-Business-Torts? Subjects-Professional&Technical-Law-Business-Torts? Selected Federal Taxation Statutes and Regulations 1997 West Group? Michael D. Rose? WestGroup? MichaelD.Rose? ジャンル別? Substores-UnknownASINs-8 Subjects-Business&Investing-Industries&Professions-Accounting-Taxes-General? Subjects-Professional&Technical-Accounting&Finance-Industries&Professions-Accounting-Taxes-General? Legal Research Exercises to Accompany How to Find the Law Finding the Law West Group? Nancy P. Johnson? WestGroup? NancyP.Johnson? ジャンル別? Substores-UnknownASINs-8 Subjects-Law? Subjects-Nonfiction-Law? Pocket Part to Local Government (Hornbooks) West Group? Osborne M. Reynolds? WestGroup? OsborneM.Reynolds? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-Series-Hornbooks? Subjects-Nonfiction-Politics-General? Subjects-Nonfiction-Law-Series-Hornbooks? Selected Commercial Statutes West Group? WestGroup? ジャンル別? Substores-UnknownASINs-8 Subjects-Business&Investing-Reference-BusinessLaw? Subjects-Law-PracticalGuides-Business? Subjects-Nonfiction-Law-PracticalGuides-Business? Listening to Music Wadsworth Pub Co? Craig Wright? WadsworthPubCo? CraigWright? ジャンル別? Substores-UnknownASINs-8 Subjects-Entertainment-Music-BooksonCD? Formats-AudioCDs-Music? Formats-AudioCDs-General? Listening to Music West Group? Craig M. Wright? WestGroup? CraigM.Wright? ジャンル別? Substores-UnknownASINs-8 Subjects-Entertainment-Music-Theory,Composition&Performance-Appreciation? Environmental Science Systems and Solutions Jones & Bartlett Pub? Michael L. McKinney? Jones&BartlettPub? MichaelL.McKinney? ジャンル別? Substores-UnknownASINs-8 Subjects-Outdoors&Nature-Environment-EnvironmentalScience Subjects-Professional&Technical-ProfessionalScience-EarthSciences-EnvironmentalScience? Subjects-Science-EarthSciences-EnvironmentalScience Subjects-Science-General Foundations of Personal Fitness Teachers Resource Binder Schools? McGraw-Hill? Schools? McGraw-Hill? ジャンル別? Substores-UnknownASINs-8 Subjects-Nonfiction-Education? College Algebra and Trigonometry West Group? David Dwyer? Mark Gruenwald? WestGroup? DavidDwyer? MarkGruenwald? ジャンル別? Substores-UnknownASINs-8 Subjects-Professional&Technical-ProfessionalScience-Mathematics-PureMathematics-Algebra-General? Subjects-Professional&Technical-ProfessionalScience-Mathematics-Trigonometry? Subjects-Science-General Subjects-Science-Mathematics-Trigonometry? Subjects-Science-Mathematics-PureMathematics-Algebra-General? Selected Environmental Law Statutes West Group? WestGroup? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-Business-Property? Subjects-Law-Environmental&NaturalResourcesLaw? Subjects-Nonfiction-Law-Business-Property? Subjects-Nonfiction-Law-Environmental&NaturalResourcesLaw? Subjects-Reference-General? Corrections West Group? Philip L. Reichel? WestGroup? PhilipL.Reichel? ジャンル別? Substores-UnknownASINs-8 Subjects-Nonfiction-Crime&Criminals-Penology? Modern Scientific Evidence The Law and Science of Expert Testimony West Pub Co? West Information Pub. Group? WestPubCo? WestInformationPub.Group? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-One-L-CivilProcedure? Subjects-Nonfiction-Law-One-L-CivilProcedure? Subjects-Professional&Technical-Law-One-L-CivilProcedure? Modern Scientific Evidence The Law and Science of Expert Testimony West Pub Co? West Information Pub. Group? WestPubCo? WestInformationPub.Group? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-One-L-CivilProcedure? Subjects-Nonfiction-Law-One-L-CivilProcedure? Subjects-Professional&Technical-Law-One-L-CivilProcedure? Modern Scientific Evidence The Law and Science of Expert Testimony West Pub Co? West Information Pub. Group? WestPubCo? WestInformationPub.Group? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-One-L-CivilProcedure? Subjects-Nonfiction-Law-One-L-CivilProcedure? Subjects-Professional&Technical-Law-One-L-CivilProcedure? Federal Civil Judicial Procedure and Rules West Group? West Information Pub Group Staff? WestGroup? WestInformationPubGroupStaff? ジャンル別? Substores-UnknownASINs-8 Subjects-Law? Subjects-Nonfiction-Law? Tennessee Rules of Court 2002 (Tennessee Rules of Court) West Group? Tennessee Courts? WestGroup? TennesseeCourts? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-Procedures&Litigation-CourtRules? Subjects-Nonfiction-Law-Procedures&Litigation-CourtRules? Subjects-Professional&Technical-Law-Procedures&Litigation-CourtRules? Patent Applications Handbook West Group? Stephen Becker? WestGroup? StephenBecker? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-IntellectualProperty-General? Subjects-Nonfiction-Law-IntellectualProperty-General? Federal Rules of Civil Procedure As Amended to May 21, 2002 (Federal Rules of Civil Procedure) West Group? WestGroup? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-General? Subjects-Nonfiction-Law-General? Subjects-Professional&Technical-Law-General? California Employment Laws 2003 West Group? West Staff? WestGroup? WestStaff? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-General? Subjects-Nonfiction-Law-General? Subjects-Professional&Technical-Law-General? Federal Labor Laws, 2002 (Selected Statutes) West Group? WestGroup? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-Business-Labor&Employment? Subjects-Nonfiction-Law-Business-Labor&Employment? Subjects-Professional&Technical-Law-Business-Labor&Employment? Federal Civil Judicial Procedure and Rules West Group? West Information Pub Group Staff? WestGroup? WestInformationPubGroupStaff? ジャンル別? Substores-UnknownASINs-8 Subjects-Law? Subjects-Nonfiction-Law? Federal Criminal Code and Rules 2002 West Group? WestGroup? ジャンル別? Substores-UnknownASINs-8 Subjects-Law? Subjects-Nonfiction-Law? California Business Statistic 2003 West Group? West? WestGroup? West? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-General? Subjects-Nonfiction-Law-General? Subjects-Professional&Technical-Law-General? California Civil Code 2003 West Group? West Staff? WestGroup? WestStaff? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-General? Subjects-Nonfiction-Law-General? Subjects-Professional&Technical-Law-General? California Civil Procedure Stature Rules 2003 West Group? West? WestGroup? West? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-General? Subjects-Nonfiction-Law-General? Subjects-Professional&Technical-Law-General? California Code of Civil Procedure 2003 West Group? West Staff? WestGroup? WestStaff? ジャンル別? Substores-UnknownASINs-8 Subjects-Law-General? Subjects-Nonfiction-Law-General? Subjects-Professional&Technical-Law-General? Postcolonial Discourse and Changing Cultural Contexts Theory and Criticism (Contributions to the Study of World Literature) Greenwood Pub Group? Gita Rajan? Radhika Mohanram? GreenwoodPubGroup? GitaRajan? RadhikaMohanram? ジャンル別? Substores-UnknownASINs-8 Subjects-Literature&Fiction-History&Criticism-Criticism&Theory-General? Subjects-Literature&Fiction-History&Criticism-Movements&Periods-Postmodernism? Subjects-Literature&Fiction-WorldLiterature-British-General? Subjects-Nonfiction-SocialSciences-Sociology-Culture? Subjects-Nonfiction-SocialSciences-PoliticalScience-Imperialism&Independence? Substores-UnknownASINs-8 洋書
https://w.atwiki.jp/tiger/pages/4.html
TEC-/- BTK-/- double mutant T cells exhibit severely impaired T cell activitity. RLK-/-ITK-/- double mutant celles exhibit severely imparired Th2 responses. Grb2(+/-) mice disrupt T cell signaling networks and development. Dendric cells and macrophages of MEK3 deficient mice have impaired IL12 production. Bam32(-/-) B cell develop normally but have impaired T-independent antibody responses in vivo. T-cell and B-cell of RAP1A deficient mice impair integrin-mediated cell adhesion. T-cell of WASP deficient mice impair the proliferaction and antigen receptor cap formation in response to anti-CD3zeta stimulation. T-cell of SHB defective mice impair the phosphorylation of LAT and consequently the activation of MAP kinase pathways. B-cell of 3BP2 (-/-) deficient mice have defective in proliferation, cell cycle progression, PLC-gamma2 phosphorylation, calcium mobilization, NF-ATp dephosphorylation, and Erk and Jnk activation in response to BCR ligation. B-cell of Vav2(-/-) deficient mice are defective in the ability to switch immunoglobulin class. T-cell of Vav1(-/-) deficient mice exhibit impaired antigen receptor signaling. Vav1(-/-)Vav2(-/-) mice exhibit greatly reduced the mature B-cells. Vav-1-/-Vav-2-/- B cells were unresponsive to BCR-driven proliferation in vitro and to thymus-indepen-dent antigen in vivo. Fyn-deficient mice exhibit a remarkably specific lymphoid defect thymocytes are refractile to stimulation through the TCR with mitogen or antigen. Lck-deficient mice show a pronounced thymic atrophy, with a dramatic reduction in the double-positive (CD4+CD8+) thymocyte population. T cell from mice deficient in LCK is required for normal signal transduction through the TCR. T cells from mice deficient in SLAP-130/Fyb show markedly impaired proliferation. B cell of chicken deficient ITK reduce IP3 generation and phospholipase C gamma 2 tyrosine phosphorylation. T cell of mice deficient ITK reduce IP3 generation and phospholipase C gamma 1 tyrosine phosphorylation. T cell of mice deficient ITK have failure of Th2 development. Mice deficient in ITK have reduced proliferative responses to MHC stimulation and to anti-TCR cross-linking Mutations in Btk cause X-linked immunodeficiency. Gads(GRAP2) has a role in thymocyte proliferaction for maturation of T-cells. Gads(GRAP2) has a role for homeostatic proliferaction in B cells. Grap negatively regulates T-cell proliferation. Gab2 is a substrate of ZAP-70 and functions as a switch molecule toward inhibition of TCR signal transuduction. B cell signaling causes tyrosine phosphorylation of Gab1, and in turn SHP2 bind to Gab1 Gab1 phosophorylation potentiate the phosphorylation of Akt, PI3K-dependent response. RasGRP1 mediates Ras activation following TCR stimulatioin. RasGRP1 and RasGRP3 induces RAS activation in B-cell to response to T-cell stimulation. Grb2-hSos1-PLCgamma1-p36/p38-ZAP70 complexes localize in the vicinity of TCR-zeta Gads(Grap2) plays an important role in T-cell signaling via its association with SLP-76 and LAT. Lck is required for normal signal transduction through the TCR. ZAP-70 plays crucial roles in T-cell activation and development. Syk triggers cellular activation in T-cell. TEC-/- BTK-/- double mutant T cells exhibit severely impaired T cell activitity. 1 J Exp Med. 2000 Dec 4;192(11) 1611-24. Severe B cell deficiency in mice lacking the tec kinase family members Tec and Btk. Ellmeier W, Jung S, Sunshine MJ, Hatam F, Xu Y, Baltimore D, Mano H, Littman DR. Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine. wilfried.ellmeier@univie.ac.at The cytoplasmic protein tyrosine kinase Tec has been proposed to have important functions in hematopoiesis and lymphocyte signal transduction. Here we show that Tec-deficient mice developed normally and had no major phenotypic alterations of the immune system. To reveal potential compensatory roles of other Tec kinases such as Bruton's tyrosine kinase (Btk), Tec/Btk double-deficient mice were generated. These mice exhibited a block at the B220(+)CD43(+) stage of B cell development and displayed a severe reduction of peripheral B cell numbers, particularly immunoglobulin (Ig)M(lo)IgD(hi) B cells. Although Tec/Btk(null) mice were able to form germinal centers, the response to T cell-dependent antigens was impaired. Thus, Tec and Btk together have an important role both during B cell development and in the generation and/or function of the peripheral B cell pool. The ability of Tec to compensate for Btk may also explain phenotypic differences in X-linked immunodeficiency (xid) mice compared with human X-linked agammaglobulinemia (XLA) patients. Publication Types Research Support, Non-U.S. Gov't PMID 11104803 [PubMed - indexed for MEDLINE] RLK-/-ITK-/- double mutant celles exhibit severely imparired Th2 responses. 1 Nat Immunol. 2001 Dec;2(12) 1183-8. Mutation of Tec family kinases alters T helper cell differentiation. Schaeffer EM, Yap GS, Lewis CM, Czar MJ, McVicar DW, Cheever AW, Sher A, Schwartzberg PL. National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA. The Tec kinases Rlk and Itk are critical for full T cell receptor (TCR)-induced activation of phospholipase C-gamma and mitogen-activated protein kinase. We show here that the mutation of Rlk and Itk impaired activation of the transcription factors NFAT and AP-1 and production of both T helper type 1 (TH1) and TH2 cytokines. Consistent with these biochemical defects, Itk-/- mice did not generate effective TH2 responses when challenged with Schistosoma mansoni eggs. Paradoxically, the more severely impaired Rlk-/-Itk-/- mice were able to mount a TH2 response and produced TH2 cytokines in response to this challenge. In addition, Rlk-/-Itk-/- cells showed impaired TCR-induced repression of the TH2-inducing transcription factor GATA-3, suggesting a potential mechanism for TH2 development in these hyporesponsive cells. Thus, mutations that affect Tec kinases lead to complex alterations in CD4+ TH cell differentiation. Publication Types Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. PMID 11702066 [PubMed - indexed for MEDLINE] Grb2(+/-) mice disrupt T cell signaling networks and development. 1 Nat Immunol. 2001 Jan;2(1) 29-36. Disruption of T cell signaling networks and development by Grb2 haploid insufficiency. Gong Q, Cheng AM, Akk AM, Alberola-Ila J, Gong G, Pawson T, Chan AC. Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, MO 63110, USA. The developmental processes of positive and negative selection in the thymus shape the T cell antigen receptor (TCR) repertoire and require the integration of multiple signaling networks. These networks involve the efficient assembly of macromolecular complexes and are mediated by multimodular adaptor proteins that permit the functional integration of distinct signaling molecules. We show here that decreased expression of the adaptor protein Grb2 in Grb2+/- mice weakens TCR-induced c-Jun N-terminal kinase (JNK) and p38, but not extracellular signal-regulated kinase (ERK), activation. In turn, this selective effect decreases the ability of thymocytes to undergo negative, but not positive, selection. We also show that there are differences in the signaling thresholds of the three mitogen-activated protein kinase (MAPK) families. These differences may provide a mechanism by which quantitative differences in signal strength can alter the balance of downstream signaling pathways to induce the qualitatively distinct biological outcomes of proliferation, differentiation or apoptosis. PMID 11135575 [PubMed - indexed for MEDLINE] Dendric cells and macrophages of MEK3 deficient mice have impaired IL12 production. 1 EMBO J. 1999 Apr 1;18(7) 1845-57. Defective IL-12 production in mitogen-activated protein (MAP) kinase kinase 3 (Mkk3)-deficient mice. Lu HT, Yang DD, Wysk M, Gatti E, Mellman I, Davis RJ, Flavell RA. Howard Hughes Medical Institute and Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA. The p38 mitogen-activated protein kinase (MAPK) pathway, like the c-Jun N-terminal kinase (JNK) MAPK pathway, is activated in response to cellular stress and inflammation and is involved in many fundamental biological processes. To study the role of the p38 MAPK pathway in vivo, we have used homologous recombination in mice to inactivate the Mkk3 gene, one of the two specific MAPK kinases (MAPKKs) that activate p38 MAPK. Mkk3(-/-) mice were viable and fertile; however, they were defective in interleukin-12 (IL-12) production by macrophages and dendritic cells. Interferon-gamma production following immunization with protein antigens and in vitro differentiation of naive T cells is greatly reduced, suggesting an impaired type I cytokine immune response. The effect of the p38 MAPK pathway on IL-12 expression is at least partly transcriptional, since inhibition of this pathway blocks IL-12 p40 promoter activity in macrophage cell lines and IL-12 p40 mRNA is reduced in MKK3-deficient mice. We conclude that the p38 MAP kinase, activated through MKK3, is required for the production of inflammatory cytokines by both antigen-presenting cells and CD4(+) T cells. PMID 10202148 [PubMed - indexed for MEDLINE] Bam32(-/-) B cell develop normally but have impaired T-independent antibody responses in vivo. 1 Immunity. 2003 Oct;19(4) 621-32. Bam32 links the B cell receptor to ERK and JNK and mediates B cell proliferation but not survival. Han A, Saijo K, Mecklenbrauker I, Tarakhovsky A, Nussenzweig MC. Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA. Bam32 is an adaptor protein recruited to the plasma membrane upon B cell receptor (BCR) crosslinking in a phosphoinositol 3-kinase (PI3K)-dependent manner; however, its physiologic function is unclear. To determine its physiologic function, we produced Bam32-deficient mice. Bam32(-/-) B cells develop normally but have impaired T-independent antibody responses in vivo and diminished responses to BCR crosslinking in vitro. Biochemical analysis revealed that Bam32 acts in a novel pathway leading from the BCR to MAPK/ERK Kinases (MEK1/2), MAPK/ERK Kinase Kinase-1 (MEKK1), extracellular signal-regulated kinase (ERK), and c-jun NH2-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (p38). This pathway appears to be initiated by hematopoietic progenitor kinase-1 (HPK1), which interacts directly with Bam32, and differs from all previously characterized BCR signaling pathways in that it is required for normal BCR-mediated proliferation but not for B cell survival. PMID 14563325 [PubMed - indexed for MEDLINE] T-cell and B-cell of RAP1A deficient mice impair integrin-mediated cell adhesion. 1 Mol Cell Biol. 2006 Jan;26(2) 643-53. Rap1A-deficient T and B cells show impaired integrin-mediated cell adhesion. Duchniewicz M, Zemojtel T, Kolanczyk M, Grossmann S, Scheele JS, Zwartkruis FJ. Department of Computational Molecular Biology, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany. Studies in tissue culture cells have demonstrated a role for the Ras-like GTPase Rap1 in the regulation of integrin-mediated cell-matrix and cadherin-mediated cell-cell contacts. To analyze the function of Rap1 in vivo, we have disrupted the Rap1A gene by homologous recombination. Mice homozygous for the deletion allele are viable and fertile. However, primary hematopoietic cells isolated from spleen or thymus have a diminished adhesive capacity on ICAM and fibronectin substrates. In addition, polarization of T cells from Rap1-/- cells after CD3 stimulation was impaired compared to that of wild-type cells. Despite this, these defects did not result in hematopoietic or cell homing abnormalities. Although it is possible that the relatively mild phenotype is a consequence of functional complementation by the Rap1B gene, our genetic studies confirm a role for Rap1A in the regulation of integrins. PMID 16382154 [PubMed - indexed for MEDLINE] T-cell of WASP deficient mice impair the proliferaction and antigen receptor cap formation in response to anti-CD3zeta stimulation. 1 Immunity. 1998 Jul;9(1) 81-91. Wiskott-Aldrich syndrome protein-deficient mice reveal a role for WASP in T but not B cell activation. Snapper SB, Rosen FS, Mizoguchi E, Cohen P, Khan W, Liu CH, Hagemann TL, Kwan SP, Ferrini R, Davidson L, Bhan AK, Alt FW. Howard Hughes Medical Institute, Children's Hospital, Boston, Massachusetts 02115, USA. The Wiskott-Aldrich syndrome (WAS) is a human X-linked immunodeficiency resulting from mutations in a gene (WASP) encoding a cytoplasmic protein implicated in regulating the actin cytoskeleton. To elucidate WASP function, we disrupted the WASP gene in mice by gene-targeted mutation. WASP-deficient mice showed apparently normal lymphocyte development, normal serum immunoglobulin levels, and the capacity to respond to both T-dependent and T-independent type II antigens. However, these mice did have decreased peripheral blood lymphocyte and platelet numbers and developed chronic colitis. Moreover, purified WASP-deficient T cells showed markedly impaired proliferation and antigen receptor cap formation in response to anti-CD3epsilon stimulation. Yet, purified WASP-deficient B cells showed normal responses to anti-Ig stimulation. We discuss the implications of our findings regarding WASP function in receptor signaling and cytoskeletal reorganization in T and B cells and compare the effects of WASP deficiency in mice and humans. PMID 9697838 [PubMed - indexed for MEDLINE] T-cell of SHB defective mice impair the phosphorylation of LAT and consequently the activation of MAP kinase pathways. 9 Sep 24;274(39) 28050-7. Requirement of the Src homology 2 domain protein Shb for T cell receptor-dependent activation of the interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells. Lindholm CK, Gylfe E, Zhang W, Samelson LE, Welsh M. Department of Medical Cell Biology, Box 571, Biomedicum, Uppsala University, S-75123 Uppsala, Sweden. Stimulation of the T cell antigen receptor (TCR) induces tyrosine phosphorylation of numerous intracellular proteins. We have recently investigated the role of the adaptor protein Shb in the early events of T cell signaling and observed that Shb associates with Grb2, linker for activation of T cells (LAT) and the TCR zeta-chain in Jurkat cells. We now report that Shb also associates with phospholipase C-gamma1 (PLC-gamma1) in these cells. Overexpression of Src homology 2 domain defective Shb caused diminished phosphorylation of LAT and consequently the activation of mitogen-activated protein kinases was decreased upon TCR stimulation. In addition, the Shb mutant also blocked phosphorylation of PLC-gamma1 and the increase in cytoplasmic Ca(2+) following TCR stimulation. Nuclear factor for activation of T cells is a major target for Ras and calcium signaling pathways in T cells following TCR stimulation, and the overexpression of the mutant Shb prevented TCR-dependent activation of the nuclear factor for activation of T cells. Consequently, endogenous interleukin-2 production was decreased under these conditions. The results indicate a role for Shb as a link between the TCR and downstream signaling events involving LAT and PLC-gamma1 and resulting in the activation of transcription of the interleukin-2 gene. PMID 10488157 [PubMed - indexed for MEDLINE] B-cell of 3BP2 (-/-) deficient mice have defective in proliferation, cell cycle progression, PLC-gamma2 phosphorylation, calcium mobilization, NF-ATp dephosphorylation, and Erk and Jnk activation in response to BCR ligation. 1 Mol Cell Biol. 2006 Jul;26(14) 5214-25. 3BP2 deficiency impairs the response of B cells, but not T cells, to antigen receptor ligation. de la Fuente MA, Kumar L, Lu B, Geha RS. Division of Immunology, Children's Hospital, 300 Longwood Ave., Boston, MA 02115, USA. The adapter protein 3BP2 is expressed in lymphocytes; binds to Syk/ZAP-70, Vav, and phospholipase C-gamma (PLC-gamma); and is thought to be important for interleukin-2 gene transcription in T cells. To define the role of 3BP2 in lymphocyte development and function, we generated 3BP2-deficient mice. T-cell development, proliferation, cytokine secretion, and signaling in response to T-cell receptor (TCR) ligation were all normal in 3BP2(-/-) mice. 3BP2(-/-) mice had increased accumulation of pre-B cells in the bone marrow and a block in the progression of transitional B cells in the spleen from the T1 to the T2 stage, but normal numbers of mature B cells. B-cell proliferation, cell cycle progression, PLC-gamma2 phosphorylation, calcium mobilization, NF-ATp dephosphorylation, and Erk and Jnk activation in response to B-cell receptor (BCR) ligation were all impaired. These results suggest that 3BP2 is important for BCR, but not for TCR signaling. PMID 16809760 [PubMed - indexed for MEDLINE] B-cell of Vav2(-/-) deficient mice are defective in the ability to switch immunoglobulin class. 1 Nat Immunol. 2001 Jun;2(6) 542-7. Comment in Nat Immunol. 2001 Jun;2(6) 482-4. Signal transduction through Vav-2 participates in humoral immune responses and B cell maturation. Doody GM, Bell SE, Vigorito E, Clayton E, McAdam S, Tooze R, Fernandez C, Lee IJ, Turner M. Laboratory of Lymphocyte Signaling and Development, Molecular Immunology Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK. B and T lymphocytes develop normally in mice lacking the guanine nucleotide exchange factor Vav-2. However, the immune responses to type II thymus-independent antigen as well as the primary response to thymus-dependent (TD) antigen are defective. Vav-2-deficient mice are also defective in their ability to switch immunoglobulin class, form germinal centers and generate secondary immune responses to TD antigens. Mice lacking both Vav-1 and Vav-2 contain reduced numbers of B lymphocytes and display a maturational block in the development of mature B cells. B cells from Vav-1(-/-)Vav-2(-/-) mice respond poorly to antigen receptor triggering, both in terms of proliferation and calcium release. These studies show the importance of Vav-2 in humoral immune responses and B cell maturation. PMID 11376342 [PubMed - indexed for MEDLINE] T-cell of Vav1(-/-) deficient mice exhibit impaired antigen receptor signaling. 1 Nature. 1995 Mar 30;374(6521) 474-7. Defective T-cell receptor signalling and positive selection of Vav-deficient CD4+ CD8+ thymocytes. Fischer KD, Zmuldzinas A, Gardner S, Barbacid M, Bernstein A, Guidos C. Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada. During lymphocyte development, cellular proliferation and positive and negative selection events ensure the production of T and B lymphocytes bearing highly diverse, but self-tolerant, repertoires of antigen receptors. These processes are initiated when engagement of growth-factor receptors, or the T and B lymphocyte antigen receptors, induces tyrosine phosphorylation of specific SH2- and SH3-domain-containing cytoplasmic proteins, including Vav. Here we show that vav-/- embryonic stem cells generate only limited numbers of immature and mature T and B lymphocytes in the RAG-2 blastocyst complementation assay. Furthermore, Vav-deficient T lymphocytes showed severely impaired antigen receptor signalling. Finally, we demonstrate that Vav-dependent signalling pathways regulate maturation, but not CD4/CD8 lineage commitment, during T-cell-receptor-mediated positive selection of immature CD4+ CD8+ precursors into mature CD4+ CD8- or CD4- CD8+ T cells. PMID 7700360 [PubMed - indexed for MEDLINE] Vav1(-/-)Vav2(-/-) mice exhibit greatly reduced the mature B-cells. Vav-1-/-Vav-2-/- B cells were unresponsive to BCR-driven proliferation in vitro and to thymus-indepen-dent antigen in vivo. 1 Nat Immunol. 2001 Jun;2(6) 548-55. Comment in Nat Immunol. 2001 Jun;2(6) 482-4. Compensation between Vav-1 and Vav-2 in B cell development and antigen receptor signaling. Tedford K, Nitschke L, Girkontaite I, Charlesworth A, Chan G, Sakk V, Barbacid M, Fischer KD. Abteilung Physiologische Chemie, Universitat Ulm, Albert-Einstein-Allee 11, D-89069 Ulm, Germany. Vav-1 and Vav-2 are closely related Dbl-homology GTP exchange factors (GEFs) for Rho GTPases. Mutation of Vav-1 disrupts T cell development and T cell antigen receptor-induced activation, but has comparatively little effect on B cells. We found that combined deletion of both Vav-1 and Vav-2 in mice resulted in a marked reduction in mature B lymphocyte numbers. Vav-1(-/-)Vav-2(-/-) B cells were unresponsive to B cell antigen receptor (BCR)-driven proliferation in vitro and to thymus-independent antigen in vivo. BCR-stimulated intracellular calcium mobilization was greatly impaired in Vav-1(-/-)Vav-2(-/-) B cells. These findings establish a role for Vav-2 in BCR calcium signaling and reveal that the Vav family of GEFs is critical to B cell development and function. PMID 11376343 [PubMed - indexed for MEDLINE] Fyn-deficient mice exhibit a remarkably specific lymphoid defect thymocytes are refractile to stimulation through the TCR with mitogen or antigen. 1 Cell. 1992 Sep 4;70(5) 751-63. Defective T cell receptor signaling in mice lacking the thymic isoform of p59fyn. Appleby MW, Gross JA, Cooke MP, Levin SD, Qian X, Perlmutter RM. Howard Hughes Medical Institute, Department of Immunology, University of Washington, Seattle 98195. Considerable evidence supports the hypothesis that the nonreceptor protein tyrosine kinase p59fyn participates in signal transduction from the T cell receptor (TCR). To examine this hypothesis in detail, we have produced mice that lack the thymic isoform of p59fyn but retain expression of the brain isoform of the protein. fynTnull mice exhibit a remarkably specific lymphoid defect thymocytes are refractile to stimulation through the TCR with mitogen or antigen, while peripheral T cells, following what appears to be a normal maturation sequence, reacquire significant signaling capabilities. These data confirm that p59fynT plays a pivotal role in TCR signal transduction and demonstrate that additional developmentally regulated signaling components also contribute to TCR-induced lymphocyte activation. PMID 1516132 [PubMed - indexed for MEDLINE] Lck-deficient mice show a pronounced thymic atrophy, with a dramatic reduction in the double-positive (CD4+CD8+) thymocyte population. 1 Nature. 1992 May 14;357(6374) 161-4. Comment in Nature. 1993 Jan 21;361(6409) 213. Profound block in thymocyte development in mice lacking p56lck. Molina TJ, Kishihara K, Siderovski DP, van Ewijk W, Narendran A, Timms E, Wakeham A, Paige CJ, Hartmann KU, Veillette A, et al. Ontario Cancer Institute, University of Toronto, Canada. The protein Lck (p56lck) has a relative molecular mass of 56,000 and belongs to the Src family of tyrosine kinases. It is expressed exclusively in lymphoid cells, predominantly in thymocytes and peripheral T cells. Lck associates specifically with the cytoplasmic domains of both CD4 and CD8 T-cell surface glycoproteins and interacts with the beta-chain of the interleukin-2 receptor, which implicates Lck activity in signal transduction during thymocyte ontogeny and activation of mature T cells. Here we generate an lck null mutation by homologous recombination in embryonic stem cells to evaluate the role of p56lck in T-cell development and activation. Lck-deficient mice show a pronounced thymic atrophy, with a dramatic reduction in the double-positive (CD4+CD8+) thymocyte population. Mature, single-positive thymocytes are not detectable in these mice and there are only very few peripheral T cells. These results illustrate the crucial role of this T-cell-specific tyrosine kinase in the thymocyte development. PMID 1579166 [PubMed - indexed for MEDLINE] T cell from mice deficient in LCK is required for normal signal transduction through the TCR. 1 Cell. 1992 Aug 21;70(4) 585-93. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Straus DB, Weiss A. Howard Hughes Medical Institute, Department of Medicine, University of California, San Francisco 94143. Signaling through the T cell antigen receptor (TCR) results both in rapid increases in tyrosine phosphorylation on a number of proteins and in the activation of the phosphatidylinositol pathway. It is not clear how stimulation of the TCR leads to these signaling events. Mutants of the Jurkat T cell line have been previously isolated that fail to show increases in calcium following receptor stimulation. Analysis of one of these mutants, JCaM1, which is defective in the induction of tyrosine phosphorylation, revealed a defect in the expression of functional lck tyrosine kinase. The lack of lck activity was caused in part by a splicing defect. Expression of the lck cDNA in JCaM1 restores the ability of the cell to respond to TCR stimulation. These results indicate that lck is required for normal signal transduction through the TCR. PMID 1505025 [PubMed - indexed for MEDLINE] T cells from mice deficient in SLAP-130/Fyb show markedly impaired proliferation. 1 Science. 2001 Sep 21;293(5538) 2263-5. Coupling of the TCR to integrin activation by Slap-130/Fyb. Peterson EJ, Woods ML, Dmowski SA, Derimanov G, Jordan MS, Wu JN, Myung PS, Liu QH, Pribila JT, Freedman BD, Shimizu Y, Koretzky GA. The Abramson Family Cancer Research Institute, Department of Medicine, School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA. SLAP-130/Fyb (SLP-76-associated phosphoprotein or Fyn-binding protein; also known as Fyb/Slap) is a hematopoietic-specific adapter, which associates with and modulates function of SH2-containing leukocyte phosphoprotein of 76 kilodaltons (SLP-76). T cells from mice lacking SLAP-130/Fyb show markedly impaired proliferation following CD3 engagement. In addition, the T cell receptor (TCR) in SLAP-130/Fyb mutant cells fails to enhance integrin-dependent adhesion. Although TCR-induced actin polymerization is normal, TCR-stimulated clustering of the integrin LFA-1 is defective in SLAP-130/Fyb-deficient cells. These data indicate that SLAP-130/Fyb is important for coupling TCR-mediated actin cytoskeletal rearrangement with activation of integrin function, and for T cells to respond fully to activating signals. PMID 11567141 [PubMed - indexed for MEDLINE] B cell of chicken deficient ITK reduce IP3 generation and phospholipase C gamma 2 tyrosine phosphorylation. 1 J Exp Med. 1996 Jul 1;184(1) 31-40. A role for Bruton's tyrosine kinase in B cell antigen receptor-mediated activation of phospholipase C-gamma 2. Takata M, Kurosaki T. Department of Oncology and Immunology, Wyeth-Ayerst Research, Pearl River, New York 10965, USA. Defects in the gene encoding Bruton's tyrosine kinase (Btk) result in a disease called X-linked agammaglobulinemia, in which there is a profound decrease of mature B cells due to a block in B cell development. Recent studies have shown that Btk is tyrosine phosphorylated and activated upon B cell antigen receptor (BCR) stimulation. To elucidate the functions of this kinase, we examined BCR signaling of DT40 B cells deficient in Btk. Tyrosine phosphorylation of phospholipase C (PLC)-gamma 2 upon receptor stimulation was significantly reduced in the mutant cells, leading to the loss of both BCR-coupled phosphatidylinositol hydrolysis and calcium mobilization. Pleckstrin homology and Src-homology 2 domains of Btk were required for PLC-gamma 2 activation. Since Syk is also required for the BCR-induced PLC-gamma 2 activation, our findings indicate that PLC-gamma 2 activation is regulated by Btk and Syk through their concerted actions. PMID 8691147 [PubMed - indexed for MEDLINE] T cell of mice deficient ITK reduce IP3 generation and phospholipase C gamma 1 tyrosine phosphorylation. 1 J Exp Med. 1998 May 18;187(10) 1721-7. T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells. Liu KQ, Bunnell SC, Gurniak CB, Berg LJ. Program of Immunology, Division of Medical Sciences, Harvard University, Boston, Massachusetts 02115, USA. Itk, a Tec family tyrosine kinase, plays an important but as yet undefined role in T cell receptor (TCR) signaling. Here we show that T cells from Itk-deficient mice have a TCR-proximal signaling defect, resulting in defective interleukin 2 secretion. Upon TCR stimulation, Itk-/- T cells release normal amounts of calcium from intracellular stores, but fail to open plasma membrane calcium channels. Since thapsigargin-induced store depletion triggers normal calcium entry in Itk-/- T cells, an impaired biochemical link between store depletion and channel opening is unlikely to be responsible for this defect. Biochemical studies indicate that TCR-induced inositol 1,4,5 tris-phosphate (IP3) generation and phospholipase C gamma1 tyrosine phosphorylation are substantially reduced in Itk-/- T cells. In contrast, TCR-zeta and ZAP-70 are phosphorylated normally, suggesting that Itk functions downstream of, or in parallel to, ZAP-70 to facilitate TCR-induced IP3 production. These findings support a model in which quantitative differences in cytosolic IP3 trigger distinct responses, and in which only high concentrations of IP3 trigger the influx of extracellular calcium. PMID 9584150 [PubMed - indexed for MEDLINE] T cell of mice deficient ITK have failure of Th2 development. 1 Immunity. 1999 Oct;11(4) 399-409. Impaired NFATc translocation and failure of Th2 development in Itk-deficient CD4+ T cells. Fowell DJ, Shinkai K, Liao XC, Beebe AM, Coffman RL, Littman DR, Locksley RM. Department of Medicine, University of California San Francisco 94143, USA. Naive Itk-deficient CD4+ T cells were unable to establish stable IL-4 production, even when primed in Th2-inducing conditions. In contrast, IFNgamma production was little affected. Failure to express IL-4 occurred even among cells that had gone through multiple cell divisions and was associated with a delay in the kinetics and magnitude of NFATc nuclear localization. IL-4 production was restored genetically by retroviral reconstitution of Itk or biochemically by augmenting the calcium flux with ionomycin. In vivo, Itk-deficient mice were unable to establish functional Th2 cells. Development of protective Th1 cells was unimpeded. These data define a nonredundant role for Itk in modulating signals from the TCR/CD28 pathways that are specific for the establishment of stable IL-4 but not IFNgamma expression. PMID 10549622 [PubMed - indexed for MEDLINE] Mice deficient in ITK have reduced proliferative responses to MHC stimulation and to anti-TCR cross-linking 1 Immunity. 1995 Dec;3(6) 757-69. Altered T cell receptor signaling and disrupted T cell development in mice lacking Itk. Liao XC, Littman DR. Department of Microbiology and Immunology, University of California, San Francisco 94143-0414, USA. Itk is a T cell protein tyrosine kinase (PTK) that, along with Btk and Tec, belongs to a family of cytoplasmic PTKs with N-terminal pleckstrin homology domains. Btk plays a critical role in B lymphocyte development. To determine whether Itk has an analogous role in T lymphocytes, we used gene targeting to prepare mice lacking expression of Itk. Such animals had decreased numbers of mature thymocytes, an effect most clearly observed in mice expressing T cell receptor (TCR) transgenes. Mature T cells from Itk-deficient mice had reduced proliferative responses to allogeneic MHC stimulation and to anti-TCR cross-linking, but responded normally to stimulation with phorbol ester plus ionomycin or with IL-2. These results provide genetic evidence that Itk is involved in T cell development and also suggest that Itk has an important role in proximal events in TCR-mediated signaling pathways. PMID 8777721 [PubMed - indexed for MEDLINE] Mutations in Btk cause X-linked immunodeficiency. 1 Semin Immunol. 1998 Aug;10(4) 309-16. Btk function in B cell development and response. Satterthwaite AB, Li Z, Witte ON. Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90095-1662, USA. Mutations in Bruton's tyrosine kinase (Btk) result in the B cell immunodeficiencies XLA in humans and Xid in mice. Both the maintenance of peripheral B cell numbers and their response to B cell antigen receptor (BCR) crosslinking depend on Btk. Btk integrates signals from multiple cell surface receptors, including BCR and G-protein coupled receptors. These Btk dependent signals control B cell proliferation and survival by mediating Ca2+ flux, activating JNK and p38 and inducing cell cycle regulatory genes. Publication Types Review PMID 9695187 [PubMed - indexed for MEDLINE] Gads(GRAP2) has a role in thymocyte proliferaction for maturation of T-cells. 1 Science. 2001 Mar 9;291(5510) 1987-91. Requirement for the SLP-76 adaptor GADS in T cell development. Yoder J, Pham C, Iizuka YM, Kanagawa O, Liu SK, McGlade J, Cheng AM. Medical Scientist Training Program, Washington University School of Medicine, St. Louis, MO 63110, USA. GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADS- CD4-CD8- thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS- thymocytes failed to respond to CD3 cross-linking in vivo and were impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS- thymocytes. These observations indicate that GADS is a critical adaptor for CD3 signaling. PMID 11239162 [PubMed - indexed for MEDLINE] Gads(GRAP2) has a role for homeostatic proliferaction in B cells. 1 Eur J Immunol. 2005 Apr;35(4) 1184-92. Expression and function of the adaptor protein Gads in murine B cells. Yankee TM, Draves KE, Clark EA. Department of Immunology, University of Washington, Seattle, USA. tyankee@kumc.edu Nearly all hematopoietic receptors are dependent on adaptor proteins for the activation of downstream signaling pathways. The Gads adaptor protein is expressed in many hematopoietic tissues, including bone marrow, lymph node, and spleen. Using intracellular staining, we detected Gads protein in a number cells, including B cells, T cells, NK cells, monocytes, and plasmacytoid DC, but not in macrophages, neutrophils, or monocyte-derived DC. In the B cell compartment, Gads was first expressed after immature B cells leave the bone marrow and was down-regulated after B cell antigen receptor (BCR) ligation. Female Gads(-/-) mice had increased numbers of splenic B cells, as compared to female Gads(+/+) mice, suggesting a role for Gads in B cell homeostasis. Although B cell production and turnover of splenic B cell subsets appeared normal in Gads(-/-) mice, homeostatic proliferation was significantly impaired in Gads(-/-) B cells. Whereas BCR ligation can induce apoptosis in wild-type transitional stage 1 (T1) B cells, Gads(-/-) T1 B cells were resistant to BCR-induced apoptosis. Gads(-/-) B cells also showed increased BCR-mediated calcium mobilization. We conclude that Gads may have a negative regulatory role in signaling through survival pathways, and is necessary for normal homeostatic proliferation in B cells. PMID 15761845 [PubMed - indexed for MEDLINE] Grap negatively regulates T-cell proliferation. 1 Mol Cell Biol. 2002 May;22(10) 3230-6. Grap negatively regulates T-cell receptor-elicited lymphocyte proliferation and interleukin-2 induction. Shen R, Ouyang YB, Qu CK, Alonso A, Sperzel L, Mustelin T, Kaplan MH, Feng GS. Program in Signal Transduction Research, The Burnham Institute, La Jolla, California 92037, USA. Grb-2-related adaptor protein (Grap) is a Grb2-like SH3-SH2-SH3 adaptor protein with expression restricted to lymphoid tissues. Grap(-/-) lymphocytes isolated from targeted Grap-deficient mice exhibited enhanced proliferation, interleukin-2 production, and c-fos induction in response to mitogenic T-cell receptor (TCR) stimulation, compared to wild-type cells. Ectopic expression of Grap led to a suppression of Elk-1-directed transcription induced by the Ras/Erk pathway, without having effects on gene expression mediated by Jnk and p38 mitogen-activated protein kinases. Together, these data suggest that Grap, unlike Grb2, acts as a negative regulator of TCR-stimulated intracellular signaling by downregulating signal relay through the Ras/Erk pathway. PMID 11971956 [PubMed - indexed for MEDLINE] Gab2 is a substrate of ZAP-70 and functions as a switch molecule toward inhibition of TCR signal transuduction. 1 J Biol Chem. 2001 Nov 30;276(48) 45175-83. Epub 2001 Sep 25. Docking protein Gab2 is phosphorylated by ZAP-70 and negatively regulates T cell receptor signaling by recruitment of inhibitory molecules. Yamasaki S, Nishida K, Hibi M, Sakuma M, Shiina R, Takeuchi A, Ohnishi H, Hirano T, Saito T. Molecular Genetics, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan. To maintain various T cell responses and immune equilibrium, activation signals triggered by T cell antigen receptor (TCR) must be regulated by inhibitory signals. Gab2, an adaptor protein of the insulin receptor substrate-1 family, has been shown to be involved in the downstream signaling from cytokine receptors. We investigated the functional role of Gab2 in TCR-mediated signal transduction. Gab2 was phosphorylated by ZAP-70 and co-precipitated with phosphoproteins, such as ZAP-70, LAT, and CD3zeta, upon TCR stimulation. Overexpression of Gab2 in Jurkat cells or antigen-specific T cell hybridomas resulted in the inhibition of NF-AT activation, interleukin-2 production, and tyrosine phosphorylation. The structure-function relationship of Gab2 was analyzed by mutants of Gab2. The Gab2 mutants lacking SHP-2-binding sites mostly abrogated the inhibitory activity of Gab2, but its inhibitory function was restored by fusing to active SHP-2 as a chimeric protein. A mutant with defective phosphatidylinositol 3-kinase binding capacity also impaired the inhibitory activity, and the pleckstrin homology domain-deletion mutant revealed a crucial function of the pleckstrin homology domain for localization to the plasma membrane. These results suggest that Gab2 is a substrate of ZAP-70 and functions as a switch molecule toward inhibition of TCR signal transduction by mediating the recruitment of inhibitory molecules to the TCR signaling complex. PMID 11572860 [PubMed - indexed for MEDLINE] B cell signaling causes tyrosine phosphorylation of Gab1, and in turn SHP2 bind to Gab1 Gab1 phosophorylation potentiate the phosphorylation of Akt, PI3K-dependent response. 1 J Biol Chem. 2001 Apr 13;276(15) 12257-65. Epub 2001 Jan 22. The Gab1 docking protein links the b cell antigen receptor to the phosphatidylinositol 3-kinase/Akt signaling pathway and to the SHP2 tyrosine phosphatase. Ingham RJ, Santos L, Dang-Lawson M, Holgado-Madruga M, Dudek P, Maroun CR, Wong AJ, Matsuuchi L, Gold MR. Departments of Microbiology and Immunology and Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada. B cell antigen receptor (BCR) signaling causes tyrosine phosphorylation of the Gab1 docking protein. This allows phosphatidylinositol 3-kinase (PI3K) and the SHP2 tyrosine phosphatase to bind to Gab1. In this report, we tested the hypothesis that Gab1 acts as an amplifier of PI3K- and SHP2-dependent signaling in B lymphocytes. By overexpressing Gab1 in the WEHI-231 B cell line, we found that Gab1 can potentiate BCR-induced phosphorylation of Akt, a PI3K-dependent response. Gab1 expression also increased BCR-induced tyrosine phosphorylation of SHP2 as well as the binding of Grb2 to SHP2. We show that the pleckstrin homology (PH) domain of Gab1 is required for BCR-induced phosphorylation of Gab1 and for Gab1 participation in BCR signaling. Moreover, using confocal microscopy, we show that BCR ligation can induce the translocation of Gab1 from the cytosol to the plasma membrane and that this requires the Gab1 PH domain as well as PI3K activity. These findings are consistent with a model in which the binding of the Gab1 PH domain to PI3K-derived lipids brings Gab1 to the plasma membrane, where it can be tyrosine-phosphorylated and then act as an amplifier of BCR signaling. PMID 11278704 [PubMed - indexed for MEDLINE] RasGRP1 mediates Ras activation following TCR stimulatioin. RasGRP1 and RasGRP3 induces RAS activation in B-cell to response to T-cell stimulation. 1 Immunol Lett. 2006 May 15;105(1) 77-82. Epub 2006 Feb 20. The role of RasGRPs in regulation of lymphocyte proliferation. Coughlin JJ, Stang SL, Dower NA, Stone JC. Department of Biochemistry, University of Alberta, Edmonton, Alta., Canada T6G 2H7. RasGRP1 links TCR signaling to Ras in T cells, while both RasGRP1 and RasGRP3 link BCR signaling to Ras in B cells. T cells deficient in RasGRP1 have defective proliferative responses as do B cells deficient in both RasGRP1 and RasGRP3, confirming the importance of Ras activation in lymphocyte proliferation. While aged Rasgrp1-/- mice develop late-onset autoimmunity characterized by splenomegaly and the presence of anti-nuclear antibodies (ANA), the additional loss of RasGRP3 expression inhibits this phenotype. We show here that the autoimmunity in Rasgrp1-/- mice is T cell dependent. Compared to wildtype, Rasgrp1-/- T cells induce greater in vitro B cell proliferation that is due, at least in part, to increased production of interleukin-4 (IL-4). Rasgrp1 Rasgrp3 double mutant B cells are less responsive to this T cell stimulation. The reduced double mutant B cell proliferative response was paralleled by decreased induction of cyclin D2 upon stimulation with IL-4 and anti-IgM. Taken together these results suggest that double mutant mice fail to generate autoimmunity due to their decreased B cell cyclin D2 accumulation, and thus proliferation, in response to the elevated levels of IL-4 produced by mutant T cells. PMID 16530850 [PubMed - indexed for MEDLINE] Grb2-hSos1-PLCgamma1-p36/p38-ZAP70 complexes localize in the vicinity of TCR-zeta 1 J Biol Chem. 1995 Aug 4;270(31) 18428-36. Ligation of the T-cell antigen receptor (TCR) induces association of hSos1, ZAP-70, phospholipase C-gamma 1, and other phosphoproteins with Grb2 and the zeta-chain of the TCR. Nel AE, Gupta S, Lee L, Ledbetter JA, Kanner SB. Department of Medicine, UCLA School of Medicine 90024, USA. Signaling by the T-cell antigen receptor (TCR) involves both phospholipase C (PLC)-gamma 1 and p21ras activation. While failing to induce Shc/Grb2 association, ligation of the TCR/CD3 receptor in Jurkat T-cells induced hSos1-Grb2 complexes. In addition to hSos1, Grb2 participates in the formation of a tyrosine phosphoprotein complex that includes 145-, 95-, 70-, 54-, and 36-38-kDa proteins. p145 was identified as PLC-gamma 1 and p70 as the protein tyrosine kinase, ZAP-70. Although of the same molecular weight, p95 was not recognized by an anti-serum to p95 Vav. The SH2 domains of Grb2 and PLC-gamma 1 were required for the formation of this protein complex. In anti-CD3-treated cells, Grb2 redistributed from the cytosol to a particulate cell compartment along with p36/p38, ZAP-70, and PLC-gamma 1. Part of the Grb2 complex associated with the particulate compartment could be extracted with Nonidet P-40, while the rest was Nonidet P-40 insoluble. In both the detergent-soluble and -insoluble fractions, Grb2 coimmunoprecipitated with the zeta-chain of the TCR. Taken together, these results indicate that anti-CD3 induces Grb2-hSos1-PLC-gamma 1-p36/p38-ZAP70 complexes, which localize in the vicinity of TCR-zeta. PMID 7629168 [PubMed - indexed for MEDLINE] Gads(Grap2) plays an important role in T-cell signaling via its association with SLP-76 and LAT. 1 Curr Biol. 1999 Jan 28;9(2) 67-75. The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors. Liu SK, Fang N, Koretzky GA, McGlade CJ. Department of Medical Biophysics, University of Toronto, The Arthur and Sonia Labatt Brain Tumour Research Centre, Hospital for Sick Children, Research Institute, 555 University Ave, Toronto, Ontario M5G 1X8, Canada. BACKGROUND The adaptor protein Gads is a Grb2-related protein originally identified on the basis of its interaction with the tyrosine-phosphorylated form of the docking protein Shc. Gads protein expression is restricted to hematopoietic tissues and cell lines. Gads contains a Src homology 2 (SH2) domain, which has previously been shown to have a similar binding specificity to that of Grb2. Gads also possesses two SH3 domains, but these have a distinct binding specificity to those of Grb2, as Gads does not bind to known Grb2 SH3 domain targets. Here, we investigated whether Gads is involved in T-cell signaling. RESULTS We found that Gads is highly expressed in T cells and that the SLP-76 adaptor protein is a major Gads-associated protein in vivo. The constitutive interaction between Gads and SLP-76 was mediated by the carboxy-terminal SH3 domain of Gads and a 20 amino-acid proline-rich region in SLP-76. Gads also coimmunoprecipitated the tyrosine-phosphorylated form of the linker for activated T cells (LAT) adaptor protein following cross-linking of the T-cell receptor; this interaction was mediated by the Gads SH2 domain. Overexpression of Gads and SLP-76 resulted in a synergistic augmentation of T-cell signaling, as measured by activation of nuclear factor of activated T cells (NFAT), and this cooperation required a functional Gads SH2 domain. CONCLUSIONS These results demonstrate that Gads plays an important role in T-cell signaling via its association with SLP-76 and LAT. Gads may promote cross-talk between the LAT and SLP-76 signaling complexes, thereby coupling membrane-proximal events to downstream signaling pathways. PMID 10021361 [PubMed - indexed for MEDLINE] Lck is required for normal signal transduction through the TCR. 1 Cell. 1992 Aug 21;70(4) 585-93. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Straus DB, Weiss A. Howard Hughes Medical Institute, Department of Medicine, University of California, San Francisco 94143. Signaling through the T cell antigen receptor (TCR) results both in rapid increases in tyrosine phosphorylation on a number of proteins and in the activation of the phosphatidylinositol pathway. It is not clear how stimulation of the TCR leads to these signaling events. Mutants of the Jurkat T cell line have been previously isolated that fail to show increases in calcium following receptor stimulation. Analysis of one of these mutants, JCaM1, which is defective in the induction of tyrosine phosphorylation, revealed a defect in the expression of functional lck tyrosine kinase. The lack of lck activity was caused in part by a splicing defect. Expression of the lck cDNA in JCaM1 restores the ability of the cell to respond to TCR stimulation. These results indicate that lck is required for normal signal transduction through the TCR. PMID 1505025 [PubMed - indexed for MEDLINE] ZAP-70 plays crucial roles in T-cell activation and development. Syk triggers cellular activation in T-cell. 1 Mol Cell Biol. 1998 Mar;18(3) 1388-99. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor reconstitution studies in a ZAP-70-deficient Jurkat T-cell line. Williams BL, Schreiber KL, Zhang W, Wange RL, Samelson LE, Leibson PJ, Abraham RT. Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA. T-cell antigen receptor (TCR) engagement activates multiple protein tyrosine kinases (PTKs), including the Src family member, Lck, and the Syk-related PTK, ZAP-70. Studies in ZAP-70-deficient humans have demonstrated that ZAP-70 plays crucial roles in T-cell activation and development. However, progress toward a detailed understanding of the regulation and function of ZAP-70 during TCR signaling has been hampered by the lack of a suitable T-cell model for biochemical and genetic analyses. In this report, we describe the isolation and phenotypic characterization of a Syk- and ZAP-70-negative somatic mutant derived from the Jurkat T-cell line. The P116 cell line displays severe defects in TCR-induced signaling functions, including protein tyrosine phosphorylation, intracellular Ca2+ mobilization, and interleukin-2 promoter-driven transcription. These signaling defects were fully reversed by reintroduction of catalytically active versions of either Syk or ZAP-70 into the P116 cells. However, in contrast to ZAP-70 expression, Syk expression triggered a significant degree of cellular activation in the absence of TCR ligation. Transfection experiments with ZAP-70-Syk chimeric proteins indicated that both the amino-terminal regulatory regions and the carboxy-terminal catalytic domains of Syk and ZAP-70 contribute to the distinctive functional properties of these PTKs. These studies underscore the crucial role of ZAP-70 in TCR signaling and offer a powerful genetic model for further analyses of ZAP-70 regulation and function in T cells. PMID 9488454 [PubMed - indexed for MEDLINE]
https://w.atwiki.jp/elvis/pages/7982.html
Magic Prague Picador? Angelo Maria Ripellino? David Newton Marinelli? David Newton Marinelli? Picador? AngeloMariaRipellino? DavidNewtonMarinelli? DavidNewtonMarinelli? ジャンル別? ユーズドブック(洋書)-History? ユーズドブック(洋書)-Nonfiction? ユーズドブック(洋書)-Reference? ユーズドブック(洋書)-Travel? Substores-UnknownASINs-8 Subjects-History-Europe-CzechRepublic? Subjects-History-Europe-England-General? Subjects-History-Europe-Germany-General? Subjects-History-Europe-Ireland-General? Subjects-Nonfiction-SocialSciences-Sociology-Culture? Subjects-Reference-Writing-Travel? Subjects-Travel-Europe-CzechRepublic-Prague? Subjects-Travel-Europe-Germany-General? Subjects-Travel-Europe-Ireland-General? Subjects-Travel-Reference&Tips-TravelWriting? The Death of Faith Pan Books? Donna Leon? PanBooks? DonnaLeon? ジャンル別? ユーズドブック(洋書)-Mystery&Thrillers? Substores-UnknownASINs-8 Subjects-Mystery&Thrillers-Authors,A-Z-(L)-Leon,Donna? Advanced Calculus (Pie) Addison Wesley? Wilfred Kaplan? AddisonWesley? WilfredKaplan? ジャンル別? ユーズドブック(洋書)-Science? Substores-UnknownASINs-8 Subjects-Science-Mathematics-PureMathematics-Calculus? Pediatric Cardiac Surgery Mosby-Year Book? Constantine, M.D. Mavroudis? Carl L., M.D. Backer? Mosby-YearBook? Constantine,M.D.Mavroudis? CarlL.,M.D.Backer? ジャンル別? ユーズドブック(洋書)-Medicine? ユーズドブック(洋書)-Professional&Technical? ユーズドブック(洋書)-Science? Substores-UnknownASINs-8 Subjects-Medicine-Specialties-Pediatrics-General? Subjects-Medicine-Specialties-Surgery-Thoracic? Subjects-Medicine-InternalMedicine-Cardiology? Subjects-Professional&Technical-Medical-Medicine-InternalMedicine-Cardiology? Subjects-Professional&Technical-Medical-Medicine-Surgery-Thoracic&Vascular? Subjects-Science-Medicine-Specialties-Pediatrics-General? Subjects-Science-Medicine-Specialties-Surgery-Thoracic? Subjects-Science-Medicine-InternalMedicine-Cardiology? The Machine Gunners Macmillan Children s Books? Robert Westall? MacmillanChildren sBooks? RobertWestall? ジャンル別? ユーズドブック(洋書)-Children sBooks? ユーズドブック(洋書)-Teens? Substores-UnknownASINs-8 Subjects-Children sBooks-History&HistoricalFiction-Fiction-Military&Wars? Subjects-Teens-Authors,A-Z-(W)-Westall,Robert? The Shape of Snakes Pan Books? Minette Walters? PanBooks? MinetteWalters? ジャンル別? ユーズドブック(洋書)-Mystery&Thrillers? Substores-UnknownASINs-8 Subjects-Mystery&Thrillers-Authors,A-Z-(W)-Walters,Minette? The Love Killers Pan Books? Jackie Collins? PanBooks? JackieCollins? ジャンル別? ユーズドブック(洋書)-Literature&Fiction? Substores-UnknownASINs-8 Subjects-Literature&Fiction-General-Contemporary? The Hegel-Marx Connection Palgrave Macmillan? Tony Burns? Ian Fraser? PalgraveMacmillan? TonyBurns? IanFraser? ジャンル別? Substores-UnknownASINs-8 Subjects-Nonfiction-Philosophy-Political? Subjects-Nonfiction-SocialSciences-PoliticalScience-General? Subjects-Nonfiction-SocialSciences-PoliticalScience-PoliticalDoctrines-Marxism? The Collected Writings Palgrave Macmillan? John Maynard Keynes? D.E. Moggridge? PalgraveMacmillan? JohnMaynardKeynes? D.E.Moggridge? ジャンル別? ユーズドブック(洋書)-Business&Investing? ユーズドブック(洋書)-Nonfiction? ユーズドブック(洋書)-Professional&Technical? Substores-UnknownASINs-8 Subjects-Business&Investing-Economics? Subjects-Nonfiction-Economics? Subjects-Professional&Technical-Accounting&Finance-Economics? Into Thin Air Pan Books? Jon Krakauer? PanBooks? JonKrakauer? ジャンル別? ユーズドブック(洋書)-Biographies&Memoirs? ユーズドブック(洋書)-Outdoors&Nature ユーズドブック(洋書)-Sports? Substores-UnknownASINs-8 Subjects-Biographies&Memoirs-General? Subjects-Outdoors&Nature-OutdoorRecreation-Mountaineering&Climbing-General? Subjects-Outdoors&Nature-OutdoorRecreation-Mountaineering&Climbing-MountainClimbing? Subjects-Sports-Mountaineering-General? Subjects-Sports-Mountaineering-MountainClimbing? Fairy Dust Macmillan Children s Books? Gwyneth Rees? MacmillanChildren sBooks? GwynethRees? ジャンル別? ユーズドブック(洋書)-Children sBooks? Substores-UnknownASINs-8 Subjects-Children sBooks-Ages9-12-General? Subjects-Children sBooks-Literature-FairyTales,FolkTales&Myths-General? Beginning Algebra Addison-Wesley? Margaret L. Lial? John Hornsby? Terry McGinnis? E. John Hornsby? Addison-Wesley? MargaretL.Lial? JohnHornsby? TerryMcGinnis? E.JohnHornsby? ジャンル別? ユーズドブック(洋書)-Professional&Technical? ユーズドブック(洋書)-Science? Substores-UnknownASINs-8 Subjects-Professional&Technical-ProfessionalScience-Mathematics-PureMathematics-Algebra-General? Subjects-Science-General Subjects-Science-Mathematics-General? Subjects-Science-Mathematics-PureMathematics-Algebra-Elementary? Subjects-Science-Mathematics-PureMathematics-Algebra-General? Not a Penny More, Not a Penny Less Pan Books? Jeffrey Archer? PanBooks? JeffreyArcher? ジャンル別? ユーズドブック(洋書)-Literature&Fiction? ユーズドブック(洋書)-Mystery&Thrillers? Substores-UnknownASINs-8 Subjects-Literature&Fiction-General-Contemporary? Subjects-Literature&Fiction-GenreFiction-Action&Adventure? Subjects-Mystery&Thrillers-Authors,A-Z-(A)-Archer,Jeffrey? Social Theory, Social Policy and Ageing Open Univ Pr? Carroll Estes? Simon Biggs? Chris Phillipson? OpenUnivPr? CarrollEstes? SimonBiggs? ChrisPhillipson? ジャンル別? ユーズドブック(洋書)-Medicine? ユーズドブック(洋書)-Nonfiction? ユーズドブック(洋書)-Parenting&Families? ユーズドブック(洋書)-Science? Substores-UnknownASINs-8 Subjects-Medicine-Specialties-Geriatrics? Subjects-Nonfiction-SocialSciences-Sociology-SocialTheory? Subjects-Parenting&Families-AgingParents-General? Subjects-Science-Medicine-Specialties-Geriatrics? Freaky Dancin' Pan Books? Bez? PanBooks? Bez? ジャンル別? ユーズドブック(洋書)-Biographies&Memoirs? ユーズドブック(洋書)-Entertainment? Substores-UnknownASINs-8 Subjects-Biographies&Memoirs-Arts&Literature? Subjects-Entertainment-Music-MusicalGenres-Rock? Adobe Type Library Adobe Pr? Adobe Systems Inc.? AdobePr? AdobeSystemsInc.? ジャンル別? ユーズドブック(洋書)-Arts&Photography? ユーズドブック(洋書)-Computers&Internet? ユーズドブック(洋書)-Reference? Substores-UnknownASINs-8 Subjects-Arts&Photography-GraphicDesign-Typography? Subjects-Computers&Internet-WebDevelopment-HTML,Graphics,&Design-WebGraphics? Subjects-Computers&Internet-GraphicDesign-General? Subjects-Computers&Internet-GraphicDesign-DesktopPublishing-General? Subjects-Computers&Internet-General? Subjects-Reference? Mass Hysteria Palgrave Macmillan? Valerie Walkerdine? Lisa Blackman? PalgraveMacmillan? ValerieWalkerdine? LisaBlackman? ジャンル別? ユーズドブック(洋書)-Health,Mind&Body? ユーズドブック(洋書)-Nonfiction? Substores-UnknownASINs-8 Subjects-Health,Mind&Body-Psychology&Counseling? Subjects-Nonfiction-SocialSciences-MediaStudies? If the Spirit Moves You Picador? Justine Picardie? Picador? JustinePicardie? ジャンル別? ユーズドブック(洋書)-Health,Mind&Body? ユーズドブック(洋書)-Nonfiction? Substores-UnknownASINs-8 Subjects-Health,Mind&Body-Death&Grief-Grief&Bereavement? Subjects-Nonfiction-TrueAccounts? Brodie's Notes on Mrs. Gaskell's "North and South" (Brodies Notes) Palgrave Macmillan? Graham Handley? PalgraveMacmillan? GrahamHandley? ジャンル別? ユーズドブック(洋書)-Children sBooks? ユーズドブック(洋書)-Literature&Fiction? Substores-UnknownASINs-8 Subjects-Children sBooks-Literature-LiteraryCriticism&Collections? Subjects-Literature&Fiction-WorldLiterature-British-19thCentury? "Whitsun Weddings" and "Less Deceived" by Philip Larkin (Master Guides) Palgrave Macmillan? Andrew Swarbrick? PalgraveMacmillan? AndrewSwarbrick? ジャンル別? ユーズドブック(洋書)-Literature&Fiction? Substores-UnknownASINs-8 Subjects-Literature&Fiction-Poetry? Subjects-Literature&Fiction-WorldLiterature-British? William Wordsworth (Critical Issues) Palgrave Macmillan? John Williams? PalgraveMacmillan? JohnWilliams? ジャンル別? ユーズドブック(洋書)-Literature&Fiction? Substores-UnknownASINs-8 Subjects-Literature&Fiction-Poetry-British-19thCentury? Crime Fiction, 1800-2000 Detection, Death, Diversity Palgrave Macmillan? Stephen Thomas Knight? PalgraveMacmillan? StephenThomasKnight? ジャンル別? ユーズドブック(洋書)-Literature&Fiction? ユーズドブック(洋書)-Mystery&Thrillers? ユーズドブック(洋書)-Nonfiction? Substores-UnknownASINs-8 Subjects-Literature&Fiction-Classics-UnitedStates-General? Subjects-Literature&Fiction-General? Subjects-Literature&Fiction-History&Criticism-Criticism&Theory-General? Subjects-Literature&Fiction-WorldLiterature-British-19thCentury? Subjects-Literature&Fiction-WorldLiterature-British-20thCentury? Subjects-Mystery&Thrillers-Mystery-Reference? Subjects-Nonfiction-Crime&Criminals-Criminology? Herbs (The Garden Plant Series) Pan Books? Roger Phillips? Nicky Foy? PanBooks? RogerPhillips? NickyFoy? ジャンル別? ユーズドブック(洋書)-Home&Garden? ユーズドブック(洋書)-Science? Substores-UnknownASINs-8 Subjects-Home&Garden-Gardening&Horticulture-Herbs? Subjects-Science-BiologicalSciences-Botany-General? European Financial Reporting Adapting to a Changing World Palgrave Macmillan? John Flower? PalgraveMacmillan? JohnFlower? ジャンル別? ユーズドブック(洋書)-Business&Investing? ユーズドブック(洋書)-Nonfiction? ユーズドブック(洋書)-Professional&Technical? Substores-UnknownASINs-8 Subjects-Business&Investing-Finance-CorporateFinance? Subjects-Business&Investing-Finance-Finance&Investing? Subjects-Business&Investing-General? Subjects-Business&Investing-Industries&Professions-Accounting-Financial? Subjects-Business&Investing-Industries&Professions-Accounting-General? Subjects-Nonfiction-Politics-International-Relations? Subjects-Professional&Technical-Accounting&Finance-Industries&Professions-Accounting-Financial? Subjects-Professional&Technical-Accounting&Finance-Industries&Professions-Accounting-General? Subjects-Professional&Technical-Accounting&Finance-Accounting-General? Subjects-Professional&Technical-Accounting&Finance-Finance-General? Subjects-Professional&Technical-Accounting&Finance-Finance-CorporateFinance? Subjects-Professional&Technical-Accounting&Finance-Finance-Finance&Investing? The Origins of World War Two The Debate Continues Palgrave Macmillan? Robert W. D. Boyce? Joseph A. Maiolo? PalgraveMacmillan? RobertW.D.Boyce? JosephA.Maiolo? ジャンル別? ユーズドブック(洋書)-History? Substores-UnknownASINs-8 Subjects-History-Americas-UnitedStates-20thCentury-General? Subjects-History-Americas-UnitedStates-20thCentury-WorldWarII-General? Subjects-History-Europe-General? Subjects-History-World-General? Introducing Psychological Research Palgrave Macmillan? Philip Banyard? Andrew Grayson? PalgraveMacmillan? PhilipBanyard? AndrewGrayson? ジャンル別? ユーズドブック(洋書)-Health,Mind&Body? Substores-UnknownASINs-8 Subjects-Health,Mind&Body-Psychology&Counseling? The Guide To Minitab Release 14 Addison-Wesley? John McKenzie? Robert N. Goldman? Addison-Wesley? JohnMcKenzie? RobertN.Goldman? ジャンル別? ユーズドブック(洋書)-Computers&Internet? ユーズドブック(洋書)-Professional&Technical? ユーズドブック(洋書)-Science? Substores-UnknownASINs-8 Subjects-Computers&Internet-Networking-DataintheEnterprise-StatisticalComputing? Subjects-Professional&Technical-ProfessionalScience-Mathematics-Applied-Statistics? Subjects-Science-General Subjects-Science-Mathematics-Applied-Probability&Statistics? Harry Emerson Fosdick Persuasive Preacher (Great American Orators, No. 2) Greenwood Pub Group? Halford Ross Ryan? GreenwoodPubGroup? HalfordRossRyan? ジャンル別? Substores-UnknownASINs-8 Subjects-Nonfiction-Education-Reference-Words&Language-Communication? Subjects-Reference-Words&Language-Communication? Subjects-Reference-General? Subjects-Religion&Spirituality-Christianity-Clergy-Preaching? Subjects-Religion&Spirituality-Christianity-Protestantism-Baptist? The Spit-Shine Syndrome Organizational Irrationality in the American Field Army (Contributions in Military Studies) Greenwood Pub Group? Christopher Bassford? GreenwoodPubGroup? ChristopherBassford? ジャンル別? Substores-UnknownASINs-8 Subjects-History-Military-Weapons&Warfare? Subjects-History-MilitaryScience? Subjects-Nonfiction-Politics-General? Subjects-Science-History&Philosophy-HistoryofTechnology? The New Deal and Its Legacy Critique and Reappraisal (Contributions in American History) Greenwood Pub Group? Robert Eden? GreenwoodPubGroup? RobertEden? ジャンル別? Substores-UnknownASINs-8 Subjects-History-Americas-UnitedStates-20thCentury-General? Subjects-History-Americas-UnitedStates-General? Subjects-Nonfiction-Politics-History&Theory? The History of Cancer An Annotated Bibliography (Bibliographies and Indexes in Medical Studies) Greenwood Pub Group? James S. Olson? GreenwoodPubGroup? JamesS.Olson? ジャンル別? Substores-UnknownASINs-8 Subjects-Health,Mind&Body-General? Subjects-Medicine-InternalMedicine-Oncology-General? Subjects-Professional&Technical-Medical-Medicine-InternalMedicine-Oncology? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-History? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-General? Subjects-Science-Medicine-InternalMedicine-Oncology-General? Holocaust Literature A Handbook of Critical, Historical, and Literary Writings Greenwood Pub Group? Saul S. Friedman? GreenwoodPubGroup? SaulS.Friedman? ジャンル別? Substores-UnknownASINs-8 Subjects-History-Europe-General? Subjects-History-Europe-Germany-Holocaust? Subjects-Literature&Fiction-General? Subjects-Literature&Fiction-History&Criticism-Criticism&Theory-General? Subjects-Nonfiction-SocialSciences-Research? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-History? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-Literature? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-General? Military Periodicals United States and Selected International Journals and Newspapers (Historical Guides to the World's Periodicals and Newspapers) Greenwood Pub Group? Michael E. Unsworth? GreenwoodPubGroup? MichaelE.Unsworth? ジャンル別? Substores-UnknownASINs-8 Subjects-History-Military-General? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-History? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-Art&Photography? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-Science? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-General? Subjects-Reference-Writing-Journalism? The Black Aged in the United States A Selectively Annotated Bibliography (Bibliographies and Indexes in Afro-American and African Studies) Greenwood Pub Group? Lenwood G. Davis? GreenwoodPubGroup? LenwoodG.Davis? ジャンル別? Substores-UnknownASINs-8 Subjects-Nonfiction-SocialSciences-Gerontology? Subjects-Nonfiction-SocialSciences-SpecialGroups-EthnicStudies? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-History? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-Science? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-General? Appeasement in Europe A Reassessment of U.S. Policies (Contributions to the Study of World History, 18) Greenwood Pub Group? David F. Schmitz? Richard D. Challener? GreenwoodPubGroup? DavidF.Schmitz? RichardD.Challener? ジャンル別? Substores-UnknownASINs-8 Subjects-History-Americas-UnitedStates-20thCentury-General? Subjects-History-Europe-General? Subjects-Nonfiction-Politics-International-Relations? Telecommunications Networks Issues and Trends (American Society of Information Science) Greenwood Pub Group? Mary Ellen Jacob? GreenwoodPubGroup? MaryEllenJacob? ジャンル別? Substores-UnknownASINs-8 Subjects-Engineering-Telecommunications-General? Subjects-Professional&Technical-Engineering-Telecommunications-General? Subjects-Science-General Key Papers in Information Science Greenwood Pub Group? Belver C. Griffith? GreenwoodPubGroup? BelverC.Griffith? ジャンル別? Substores-UnknownASINs-8 Subjects-Nonfiction-SocialSciences-Library&InformationScience-General? Subjects-Reference-General? Historical Dictionary of the U.S. Air Force Greenwood Pub Group? Charles D. Bright? GreenwoodPubGroup? CharlesD.Bright? ジャンル別? Substores-UnknownASINs-8 Subjects-History-Military-Aviation? Subjects-History-Military-General? Subjects-History-Military-UnitedStates-General? Subjects-Reference? Labor and Industrial Relations Journals and Serials An Analytical Guide (Annotated Bibliographies of Serials, No 14) Greenwood Pub Group? Michael C. Vocino? Lucille W. Cameron? GreenwoodPubGroup? MichaelC.Vocino? LucilleW.Cameron? ジャンル別? Substores-UnknownASINs-8 Subjects-Reference-Publishing&Books-Bibliographies&Indexes-General? Fundamentals of Geriatrics for Health Professionals An Annotated Bibliography (Bibliographies and Indexed in Gerontology, No 11) Greenwood Pub Group? Jodi L. Teitelman? Iris A. Parham? GreenwoodPubGroup? JodiL.Teitelman? IrisA.Parham? ジャンル別? Substores-UnknownASINs-8 Subjects-Medicine-Specialties-Geriatrics? Subjects-Nonfiction-SocialSciences-Gerontology? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-Medicine? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-Science? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-General? Subjects-Science-Medicine-Specialties-Geriatrics? Europe Dream-Adventure-Reality (Documentary Reference Collections) Greenwood Pub Group? Marlene L. Rossman? GreenwoodPubGroup? MarleneL.Rossman? ジャンル別? Substores-UnknownASINs-8 Subjects-History-Europe-England-General? Subjects-History-Europe-General? Subjects-History-Europe-Ireland-General? Subjects-Nonfiction-Government-Constitutions? Spotlight on the Child Studies in the History of American Children's Theatre (Contributions in Drama and Theatre Studies) Greenwood Pub Group? Roger L. Bedard? C. John Tolch? GreenwoodPubGroup? RogerL.Bedard? C.JohnTolch? ジャンル別? Substores-UnknownASINs-8 Subjects-Arts&Photography-PerformingArts-Theater-Direction&Production? Subjects-Arts&Photography-PerformingArts-Theater-General? Subjects-Arts&Photography-PerformingArts-General? Subjects-Entertainment-PerformingArts-Theater-Direction&Production? Subjects-Entertainment-PerformingArts-Theater-General? Subjects-Literature&Fiction-Drama-History? Subjects-Literature&Fiction-History&Criticism-Criticism&Theory-General? Julie Andrews A Bio-Bibliography (Bio-Bibliographies in the Performing Arts) Greenwood Pub Group? Les Spindle? GreenwoodPubGroup? LesSpindle? ジャンル別? Substores-UnknownASINs-8 Subjects-Arts&Photography-PerformingArts? Subjects-Biographies&Memoirs-Arts&Literature? Subjects-Biographies&Memoirs-General? Subjects-Entertainment-Movies-Biographies? Subjects-Entertainment-Movies-General? Subjects-Nonfiction-Education-Reference-Words&Language-Communication? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-Music? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-Art&Photography? Subjects-Reference-Publishing&Books-Bibliographies&Indexes-General? Subjects-Reference-Words&Language-Communication? The Librarians' Guide to Microcomputer Technology and Applications Knowledge Industry Pubns? Nolan Pope? Lawrence Woods? KnowledgeIndustryPubns? NolanPope? LawrenceWoods? ジャンル別? Substores-UnknownASINs-8 Defender of the Union The Oratory of Daniel Webster (Great American Orators) Greenwood Pub Group? Craig R. Smith? GreenwoodPubGroup? CraigR.Smith? ジャンル別? Substores-UnknownASINs-8 Subjects-History-Americas-UnitedStates-19thCentury-General? Subjects-History-Americas-UnitedStates-General? Subjects-Nonfiction-Education-Reference-Words&Language-Communication? Subjects-Reference-Words&Language-Communication? Subjects-Reference-General? Essays of Robert Koch (Contributions in Military Studies) Greenwood Pub Group? K. Codell Carter? GreenwoodPubGroup? K.CodellCarter? ジャンル別? Substores-UnknownASINs-8 Subjects-Health,Mind&Body-General? Subjects-Medicine-BasicScience-Microbiology? Subjects-Medicine-InternalMedicine-InfectiousDisease? Subjects-Professional&Technical-Medical-BasicSciences-Microbiology? Subjects-Science-Medicine-BasicScience-Microbiology? Subjects-Science-Medicine-InternalMedicine-InfectiousDisease? Women's and Men's Liberation Testimonies of Spirit (Contributions in Philosophy) Greenwood Pub Group? Leonard Grob? Riffat Hassan? Haim Gordon? GreenwoodPubGroup? LeonardGrob? RiffatHassan? HaimGordon? ジャンル別? Substores-UnknownASINs-8 Subjects-Nonfiction-Philosophy-General? Subjects-Nonfiction-Women sStudies-FeministTheology? Subjects-Religion&Spirituality-Christianity-ChristianLiving-Women sIssues? Subjects-Religion&Spirituality-Christianity-Theology-General? Subjects-Religion&Spirituality-Christianity-Theology-Feminist? Subjects-Religion&Spirituality-General? Subjects-Religion&Spirituality-ReligiousStudies-ComparativeReligion? Subjects-Religion&Spirituality-Spirituality-Women? Historical Dictionary of Reconstruction Greenwood Pub Group? Hans L. Trefousse? GreenwoodPubGroup? HansL.Trefousse? ジャンル別? Substores-UnknownASINs-8 Subjects-History-Americas-UnitedStates-19thCentury-General? Subjects-History-Americas-UnitedStates-19thCentury-Reconstruction? Subjects-History-Americas-UnitedStates-CivilWar-General? Subjects-History-Americas-UnitedStates-General? Subjects-Reference? Nuclear War and Nuclear Strategy Unfinished Business Unfinished Business (Contributions in Military Studies) Greenwood Pub Group? Stephen J. Cimbala? GreenwoodPubGroup? StephenJ.Cimbala? ジャンル別? Substores-UnknownASINs-8 Subjects-History-Military-General? Subjects-History-Military-Weapons&Warfare-Nuclear? Subjects-History-MilitaryScience? Central America Historical Perspectives on the Contemporary Crises (Contributions to Study of World History, No 10) Greenwood Pub Group? Ralph Lee., Jr. Woodward? GreenwoodPubGroup? RalphLee.,Jr.Woodward? ジャンル別? Substores-UnknownASINs-8 Subjects-Business&Investing-Biographies&Primers-BusinessHistory? Subjects-History-Americas-CentralAmerica-General? Subjects-History-Americas-Mexico? Subjects-Nonfiction-Economics-EconomicHistory? Subjects-Professional&Technical-Accounting&Finance-Economics-EconomicHistory? Substores-UnknownASINs-8 洋書
https://w.atwiki.jp/armoredcoreforever/pages/348.html
チャプター00 / URBAN AREA レジスタンス部隊を追撃せよ 攻略難度 3 敵対勢力 レジスタンス部隊 敵主戦力 戦車・航空兵器 他多数 ランクS目安収支 500000 サブクエスト クエスト名 内容 報酬 獲得チームポイント 備考 戦車撃破 戦車を10両撃破 10000 10 戦闘ヘリ撃破 戦闘ヘリを10機撃破 10000 10 固定砲台撃破 砲台を10門撃破 10000 10 輸送ヘリ撃破 輸送ヘリを全て撃破 10000 10 防衛型撃破 防衛型を10機撃破 20000 20 特殊高機動型撃破 特殊高機動型を4機撃破 30000 30 チャージマスター ブーストチャージで10機撃破 20000 20 スピード撃破A 大型武装ヘリを2分以内に撃破 30000 30 スピード撃破B リーダーAC(ヴェンデッタ)を30秒以内に撃破 30000 30 廃品回収A 残骸Aを回収 10000 10 廃品回収B 残骸Bを回収 10000 10 廃品回収C 残骸Cを回収 20000 20 廃品回収D 残骸Dを回収 20000 20 時間制限 5分以内にクリア 30000 30 損害軽微 総ダメージ10000以下でクリア 30000 30 廃品回収 番号 場所 入手パーツ A 廃品Cの目印になる倒壊したビルを超えた先の高速道路の下 KO-4T5[LASER BLADE](ARM UNIT) B 廃品Cの目印になる倒壊したビル近くの地面、ビルとビルの間 URF-15 VALDOSTA[RIFLE](ARM UNIT) C スタート地点から道なりに進んでいくと見える、倒壊して道を破壊しているビルの左にあるヘリポート近く KO-2H4/PODENKA[BATTLE RIFLE](ARM UNIT) D 大型武装ヘリ出現地点付近(左側のビル沿い) USM-13 GULBARGA[CE MISSILE](SHOULDER UNIT) 敵AC ヴェンデッタ AP 32977 防御属性 KE CE TE - 2711 497 664 HEAD UHD-10 TRISTAN CORE UCR-10/A ARMS UAM-10/A LEGS ULG-10/A DENALI 装備位置 武装 攻撃属性 攻撃力 LA URF-15 VALDOSTA KE RA UEM-34 MODESTO TE SU USM-13 GULBARGA CE 攻略 機体の動きに馴れるための導入、ジャンク機体でも問題なく攻略できる。 敵は戦車、ヘリ、防衛型、固定砲台、輸送ヘリなど。突撃型と敵ACも出る。 ガトリングは精度の低さがネック。パルスマシンガンは射程が短く、ライフルは連射速度が若干遅い。それぞれの長所短所を把握しておくこと。 固い敵にはハイブースト押しっ放しのチャージが有効、スキャンモード中でも出せる。 戦車や防衛型相手にブーストチャージの練習をしておこう。 戦車は小さく背が低いためブーストチャージは意外と当てにくい。蹴り足としっかり軸をあわせよう。 固定砲台はビルの屋上などに設置されているのでビルを昇る必要がある。逆関節二脚ならば昇りやすい。 敵AC(ヴェンデッタ)はの登場フラグは ルート沿い2機目の輸送ヘリ破壊。 登場は 3機目の位置なので、スピード撃破B を狙うときはグライドで急ぐか 3機目側に通り過ぎてから 2機目を破壊するといい。 ヴェンデッタは CE に弱い。バトルライフル 2丁持ちでトリガーを引いたままロックし続けられればスピード撃破Bに十分間に合う。 ヴェンデッタはこちらを補足するまで接近、その後は引き撃ちとなる。 初期出現位置はマップ進行で最初からいる1機目の大型輸送ヘリより手前の小広場。背負っているご立派なものは抜いてこないので御安心を。 雑魚が邪魔なら、高架下に追い込めばヴェンデッタだけを狙いやすい。 トンネルを塞ぐ大型ヘリは真下に潜ればミサイルが当たらない、ビル上からの狙撃でも当たらない。 大型ヘリの弱点はCE系。手が無くてもブーストチャージで攻撃出来る。 攻撃属性はKEとCEで事足り、ミサイルも大型ヘリ以外はさほど撃ってこない。大型ヘリは真下が死角なので、潜り込んで空いた肩に積んだCEロケを連射すれば一瞬で落ちる。 トンネルを進むとムービーがあり終了。 トンネルは意外と長い。ここで下手に手間取って時間制限を取り逃がすことの無い様に。 戦闘評価S習得は、サブクエストを埋めながら損害を抑えればそれだけでOK。 チャージマスターは固定砲台や戦車を相手にするとよい。防衛型などに仕掛けると、思わぬ攻撃を受けて本末転倒になる、注意。 全サブミッション同時達成を狙うと難易度が跳ね上がる(特に時間制限)。こんなのじゃ物足りないオフ専な人はどうぞ。 コメント スピード早めにルートを行くとビルが倒れてくるところで、倒れてくるはビルに当たると大幅にAPが減る。 -- 名無しさん (2012-03-04 20 16 42) 何気に時間制限がやや厳しめ ルート表示までの会話で時間を食われるのでなくても進めるよう道を覚えておくと吉 -- 名無しさん (2012-03-04 21 42 38) 大型ヘリに近づいて(近づきすぎ注意)パルマシ撃てば爆発のおかげでミサイルが一切飛んでこない。(ミサイル発射した瞬間に爆発してしまう) -- 名無しさん (2012-03-17 22 28 37) ここの廃品をすべて回収すると、初期機体の武装の正規品がワンセット、タダで手にはいる。 -- 名無しさん (2012-03-24 14 20 02) 全サブミッション同時達成やってみたんだがクリアすんのに2日かかった・・・ -- 名無しさん (2012-04-08 03 41 11) 高架を渡り切った先のビルの倒壊、これに巻き込まれると中量機はほぼ一撃ですね……。時間制限を狙おうと渡るのをを急いてしまうと、ちょうどタイミングが合ってしまうみたいです。 -- 名無しさん (2012-05-05 22 30 16) 名前 コメント
https://w.atwiki.jp/youge_wiki/pages/35.html
Software Back to Sort ABC Sort Category FileUtil File_copier v1.0 100c 1Gq Copies programs and data from one databank to another standard software tool given free to all Agents FileUtil File_Deleter v1.0 100c 1Gq Delete programs and data from a databank standard software tool given free to all Agents FileUtil Defrag v1.0 5000c 2Gq Pushes all of your files together at the top of your memory banks and free up large blocks of space of for use FileUtil Security Firewall_Disable v1.0 2000c 1Gq Disables a firewall with a security level of 1.This action will be detected immediately Security Firewall_Disable v2.0 3000c 1Gq Disables a firewall with a security level of 2 or below..This action will be detected immediately Security Firewall_Disable v3.0 4000c 1Gq Disables a firewall with a security level of 3 or below..This action will be detected immediately Security Firewall_Disable v4.0 6000c 2Gq Disables a firewall with a security level of 4 or below.This action will be detected immediately Security Firewall_Disable v5.0 8000c 3Gq Disables a firewall with a security level of 5 or below..This action will be detected immediately Security Log_Deleter v1.0 500c 1Gq Deletes the contents of an access log from a computer leaves an empty log behind Security Log_Deleter v2.0 1000c 1Gq Deletes the contents of an access log from a computer leaves a distinctive blank space behind Security Log_Deleter v3.0 2000c 1Gq Copies another user's legitimate access log over the top of your own.very difficult to detect. Security Log_Deleter v4.0 4000c 1Gq Deletes an access from a computer Moves other logs up to cover empty space impossible to detect. Security Log_Modifier v1.0 4000c 2Gq Allows you to make modifications to an existing log Security Log_Modifier v2.0 6000c 2Gq Allows you to make modifications to an existing log,and create logs in blank spaces Security Log_undeleter v1.0 5000c 1Gq Recovers previously deleted logs Security Proxy_Disable v1.0 3000c 1Gq Disables aproxy with a security level of 1.This action will be detected immediately Security Proxy_Disable v2.0 3000c 1Gq Disables aproxy with a security level of 2 or below.This action will be detected immediately Security Proxy_Disable v3.0 6000c 1Gq Disables aproxy with a security level of 3 or below.This action will be detected immediately Security Proxy_Disable v4.0 8000c 1Gq Disables aproxy with a security level of 4 or below.This action will be detected immediately Security Proxy_Disable v5.0 10000c 1Gq Disables aproxy with a security level of 5 or below.This action will be detected immediately Security Trace_Tracker v1.0 300c 1Gq informs you on the progress of any traces on your connection(No trace detected,Trace in progress,Trace complete) Security Trace_Tracker v2.0 600c 1Gq informs you on the progress of any traces on your connection(No trace detected,Trace in progress,Trace complete) Security Trace_Tracker v3.0 1400c 2Gq informs you on the progress of any traces on your connection(No trace detected,Trace in progress,Trace complete) Security Trace_Tracker v4.0 2500c 3Gq informs you on the progress of any traces on your connection(No trace detected,Trace in progress,Trace complete)including an estimate of the exaxt time remaining Security Crackers Decrypter v1.0 800c 2Gq Decrypts any file encrypted with an Encrypter v1.0 or below Cracker Decrypter v2.0 1200c 2Gq Decrypts any file encrypted with an Encrypter v2.0 or below Cracker Decrypter v3.0 1600c 2Gq Decrypts any file encrypted with an Encrypter v3.0 or below Cracker Decrypter v4.0 2200c 2Gq Decrypts any file encrypted with an Encrypter v4.0 or below Cracker Decrypter v5.0 3000c 3Gq Decrypts any file encrypted with an Encrypter v5.0 or below Cracker Decrypter v6.0 7000c 4Gq Decrypts any file encrypted with an Encrypter v6.0 or below Cracker Decrypter v7.0 15000c 5Gq Decrypts any file encrypted with an Encrypter v7.0 or below Cracker Decypher v1.0 3000c 2Gq Breaks through Encryption Cyphers. Uses a Quadratic Decryption Algorithm to force crack an Elliptiv-curve Encryption Cypher Cracker Decypher v2.0 5000c 2Gq Breaks through Encryption Cyphers. Uses a Quadratic Decryption Algorithm to force crack an Elliptiv-curve Encryption Cypher Cracker Decypher v3.0 8000c 2Gq Breaks through Encryption Cyphers. Uses a Quadratic Decryption Algorithm to force crack an Elliptiv-curve Encryption Cypher Cracker Dictionary_Hacker v1.0 1000c 4Gq A cheap and fast method of breaking passwords.this software tool tries every rod in a ten-thousand word dictionary as a password.it is faster than a Password Breaker,but not graunted to find an answer Cracker Password_Breaker v1.0 1500c 2Gq Hack past passwor protection screens not as fast a DictionaryHacker but is guranteed to find the password eventually Cracker HUD Upgrade HUD_ConnectionAnalaysis v1.0 20000c 0Gq Upgrade your HUD so that you can see the security systems installed on your target computer,and launch Bypass software at them HUDUpgrade HUD_IRC-Client v1.0 4000c 0Gq Upgrade your HUD with a new screen,in which you can participate in internet Relay Chat.This allows you to talk to other hackers for help.You will also need an internet connection on your local machine. HUDUpgrade HUD_MapShowTrace v1.0 5000c 0Gq Upgrade your HUD so that your map shos the progress of any your connection.Replace the Trace Tracker HUDUpgrade Hud_LANView v1.0 5000c 0Gq Uograde your HUD so that you can analyse and connect to Local Area Network(LAN) systems.this upgrade is required you can install an run any LAN software. HUDUpgrade Bypassers Firewall_Bypass v1.0 3000c 1Gq Actively bypasses firewall systems with a security level of 1.bypassers require a'HUD connectionAnalaysis upgrade first Bypasser Firewall_Bypass v2.0 4000c 1Gq Actively bypasses firewall systems with a security level of 2 or below.bypassers require a'HUD connectionAnalaysis upgrade first Bypasser Firewall_Bypass v3.0 6000c 1Gq Actively bypasses firewall systems with a security level of 3 or below.bypassers require a'HUD connectionAnalaysis upgrade first Bypasser Firewall_Bypass v4.0 8000c 1Gq Actively bypasses firewall systems with a security level of 4 or below.bypassers require a'HUD connectionAnalaysis upgrade first Bypasser Firewall_Bypass v5.0 10000c 1Gq Actively bypasses firewall systems with a security level of 5 or below.bypassers require a'HUD connectionAnalaysis upgrade first Bypasser Monitor_Bypass v1.0 10000c 1Gq Active bypasses a monitor with a security level of 1.All bypassers require a'HUD connectionAnalaysis upgrade first Bypasser Monitor_Bypass v2.0 12000c 1Gq Active bypasses a monitor with a security level of 2 or below.All bypassers require a'HUD connectionAnalaysis upgrade first Bypasser Monitor_Bypass v3.0 16000c 1Gq Active bypasses a monitor with a security level of 3 or below.All bypassers require a'HUD connectionAnalaysis upgrade first Bypasser Monitor_Bypass v3.0 16000c 1Gq Active bypasses a monitor with a security level of 4 or below.All bypassers require a'HUD connectionAnalaysis upgrade first Bypasser Monitor_Bypass v3.0 16000c 1Gq Active bypasses a monitor with a security level of 5 or below.All bypassers require a'HUD connectionAnalaysis upgrade first Bypasser Proxy_Bypass v1.0 6000c 1Gq Active bypasses a proxy server with a Security level of 1.All bypassers require a'HUD connectionAnalaysis upgrade first HUDUpgrade Proxy_Bypass v2.0 8000c 1Gq Active bypasses a proxy server with a Security level of 2 or below.All bypassers require a'HUD connectionAnalaysis upgrade first HUDUpgrade Proxy_Bypass v3.0 12000c 1Gq Active bypasses a proxy server with a Security level of 3 or below.All bypassers require a'HUD connectionAnalaysis upgrade first HUDUpgrade Proxy_Bypass v4.0 16000c 1Gq Active bypasses a proxy server with a Security level of 4 or below.All bypassers require a'HUD connectionAnalaysis upgrade first HUDUpgrade Proxy_Bypass v1.0 20000c 1Gq Active bypasses a proxy server with a Security level of 5 or below.All bypassers require a'HUD connectionAnalaysis upgrade first HUDUpgrade LAN tools LAN_Probe v1.0 15000c 3Gq Scans a single system on a LAN,and the links running from it LAN LAN_Probe v2.0 20000c 3Gq Scans a single system on a LAN,and the links running from it LAN LAN_Probe v3.0 30000c 4Gq Scans a single system on a LAN,and the links running from it LAN LAN_Scan v1.0 10000c 2Gq Scans an entire LAN,looking for systems LAN LAN_Scan v2.0 15000c 2Gq Scans an entire LAN,looking for systems LAN LAN_Scan v3.0 25000c 2Gq Scans an entire LAN,looking for systems LAN LAN_Spoof v1.0 20000c 2Gq Spoofs a system on the network,fooling the network itself into believing you are that system.Works on level 1system only. LAN LAN_Spoof v2.0 30000c 3Gq Spoofs a system on the network,fooling the network itself into believing you are that system.Works on level 2and 2 systems. LAN LAN_Spoof v3.0 45000c 4Gq Spoofs a system on the network,fooling the network itself into believing you are that system.Works on level all LAN systems. LAN LAN_Force v1.0 15000c 2Gq This tool will force open any lock system you might encounter on a LAN system.The sysAdmin will probably notice this LAN LAN_Force v2.0 20000c 3Gq This tool will force open any lock system you might encounter on a LAN system.The sysAdmin will probably notice this LAN LAN_Force v3.0 25000c 4Gq This tool will force open any lock system you might encounter on a LAN system.The sysAdmin will probably notice this LAN Other IP_Lookup v1.0 500c 1Gq Converts a raw IP number into internet address and adds it to your links Other IP_Probe v1.0 2000c 3Gq Scans a given IP address for security systems and reports the types in use Other IP_Probe v2.0 4000c 3Gq Scans a given IP address for security systems and reports the types in use.their version information Other IP_Probe v3.0 5000c 3Gq Scans a given IP address for security systems and reports the types in use.their version information and their Status Other voice_Analyser v1.0 5000c 4Gq Recors and analyses voice patterns for reproduction.Can be used to simulate voice authorisations. Other voice_Analyser v2.0 10000c 5Gq Recors and analyses voice patterns for reproduction.Can store load voice to and from gateway memory.Can used to simurate voice authorisation. Other
https://w.atwiki.jp/nighthorizon2/pages/63.html
vendicatore (語り) 月も星も見えない夜 闇に佇むひとつの影 故郷を失くした悲しい男の物語 農民(coltivatore)の子に生まれ 農民(coltivatore)として育ち そして農民(coltivatore)の娘(こ)と契る 幸せ(Felice)を謡い 朽ちていく運命(Destino) それが少年の未来・・・そのはずだった (A) 嵐と共に奴等はやってくる 神の悪戯か? 気づけないうちに 家族(3人)は闇に呑まれて (語り) 少年は 母親に手を握られ眠りにおちている (A ) やさしいmamma 強いpapa 未来(明日)は黒い絶望だというのに こんなに幸せな表情(かお)でいられるのか (A) かすかな気配を感じて 少年は目を覚ます 両脇には優しい両親 静寂を支配するはずの闇が揺らぎ始める (A ) まずはGrave そしてVivace 大地を駆ける 漆黒の闇を切り裂きながら 馬(cavallo)に跨り 奴らはやってくる (B) 母親を起こす少年 次第に 恐怖に 喰われる感覚が体を支配する いつかの日の記憶がよみがえり 動悸は激しく胸を打つ (B ) そして母親は父親を起こす頃 村(villaggio)に恐怖の声が上がり始める (B ) 父親が目を覚ます頃 離れた場所で 幼馴染(Liceリーチェ)の声が遠くで聞こえた (語り) 息を呑む家族 幾許かの沈黙の後・・・ 扉は蹴破られた (サビ) どこかで聞こえる悲鳴は混じり それは自分のものか 母親のものか わけもわからず叫び続けた 狂う神経 ↓ 鬼の形相 ↓ 歪む視界 ↓ 父親(血)の臭い ↓ 裂かれる布 ↓ 白い肌 (C) やがて叫ぶことをやめてしまう コマ送りの惨劇は終り始め 主催者(finanziatore)は新しい惨劇を上演する (C’) 奴らは少年に手を伸ばし 少年の精神(こころ)を奪った (C) 生臭いにおいが口に広がる 父親の死も 母親の揺れる裸体も 自分に振りかかる災厄を 遠い感覚で見ていた (C’) 切り離された精神(こころ)は 裂ける痛みで目を覚ます (サビ) どこかで聞こえる悲鳴は混じり それは自分のものか 母親のものか わけもわからず叫び続けた 獣の真似事 ↓ 足を伝う血 ↓ 息苦しい口 ↓ 穢される肢体 ↓ 奴らのtatoo ↓ 壊される精神(こころ) (セリフ) 「こんなに痛いのに、こんなに辛いのに なぜ奴らは嬉しそうなの?」 激しく揺さぶられた後 唐突に束の間の終わりがやって来る (D) 2度、3度と繰り返された惨劇の後 少年は命を乞う 奴らの一人が言った 「・・・・・・・」 (語り) そして奴らの怒号が上がる (セリフ) 今までありがとう 大好きなmamma papa だけどごめんね 僕は・・・僕は・・・ (語り) 恐怖は人を狂わせる 軽々と向こう側へ人を運ぶ 血濡れのナイフに刺さるmamma 柔らかな胸に吸い込まれたナイフ 優しい瞳をしたmammaは少年の手を包み込んでいた それを満足そうに奴らはみていた (E) 呆然と座り込む少年 奴らは荒らしつくした我が家を後に出て行く この家に残されたのは 2つの死体と一人のvendicatore (E) 両親の小さな願いは叶った 母親は死の間際に思った「どうかあの子に幸せ(Felice)を・・・」 しかし未来(あす)は知っている 彼の幸せは終ってしまったのだと (語り) 数日後、村から少年は姿を消す そして月日は流れ彼の復讐劇の幕が開く Una tragedia(悲劇) La Sorte(運命) vendicatore(復讐者) (セリフ) 「みつけたぞ。まずは奴らの・・・。」
https://w.atwiki.jp/linsivvi/pages/164.html
Into the Heart of Mistmoore ■BBM 1. The Stone Behind the Ankhの最後でSir Trodonisに報告してから約5分後からクエストが受けられるようになる ■ミストムーア地下墓地 1. ミストムーア地下墓地(-86,-31,83)にるドワーフに話しかける 2. (-67,-31,95)にある巻物を読みドワーフを正気に戻す方法を調べる 3. Head Maid Zyra か A Burning Vampireを倒しDust of Recollectionを手に入れる 4. 同じ部屋にあるLibation Wineryからblood of the Thrallを4つ採集する 5. The Blood ForgeにいるMobからEvil Eye Bileを4つ手に入れる 6. (-63,-28,-93)でStein of Cohesionを手に入れる 7. (-166,-30,77)でCauldron of Uzunと話しLiberation Libationを作ってもらう 8. (-86,-31,83)に戻りドワーフにLiberation Libationを飲ませるとドワーフは 隣の部屋に走っていくので追いかけて話しかける 9. The Blood Forgeに行きトロルを倒す(PHでも可) 10.城に上がる階段の所でSeneschal D'naraz かPHのa Mistmoore aideを倒す ■MMC 1.(-90,0,-20)の箱を調べて本を拾う 2. Eternal Bloom bookのページを集める Page #3 (-156,-4,-88) (Kormak Syncal room) Page #5 (-74,5,-50) (Sage Room) Page #7 (-244,6,-17) (Expirimentation Chamber Page #8 (-135,-5,30) (Fountain room) Page #10 (-20,4,56) (Graf Koban room) Page #2 (-135,0,-48) (Room between ballroom and Vikmont) 3.-87,0,8の小部屋にあるドクロの燭台を調べる 4.ミストモアの錆を10個集めて、もう一度ドクロの燭台を調べた後本を読む 5.地下墓地から上がってくる螺旋階段の部屋の壁に書かれている絵(-189,-9,-12)を調べる 6.9体のNAMEDを倒す 7.-105,-5,57にあるMayongの銅像を調べ、Blade of Mistmyrを手に入れる 8.-90,0,20にある箱を調べて Soulfire's bladeを手に入れる 9.BBMのSir Trodonisに報告して終了 A Freeport Overlord in Queen Lenya s Courtへ続く
https://w.atwiki.jp/akasatanahama/pages/115.html
鉱石から鉱石ブロックをドロップする方法くぉおあいえていただけないでsy - World_of_the_Takumi 2016-03-11 07 40 22 AluminiumOreクラスで、getItemDropped・quantityDroppedWithBonus・getExpDropの部分を削除すればできます。 - 赤砂蛇凪浜 2016-03-13 07 44 13 返信ありがとうございました、わかりにくいコメントですいません。今後から気を付けます。あともう一つお願いします" new WorldGenMinable(AluminiumMod.oreAluminium, 0, 20, Blocks.stone).generate(world,”以降はどうなっていますか。wikiにものってなかったのでお願いします。 - World_of_the_Takumi 2016-03-13 19 18 57 ソースコードの表示が見切れてしまっていたでしょうか。このようになっています。new WorldGenMinable(AluminiumMod.oreAluminium, 0, 20, Blocks.stone).generate(world, random, genX, genY, genZ); - 赤砂蛇凪浜 2016-03-14 06 40 37 ありがとうございました。感謝します - World_of_the_Takumi 2016-03-14 17 38 44 はじめまして このような素晴らしいwikiをありがとうございます 一つ気になることがあるのですが AluminiumOreGenerator.javaの22行目 「for(int i = 0; i 64; i++) {」 26行目 「new WorldGenMinable(decorationMOD.oreDecoration, 0, 64, Blocks.stone).generate(world, random, genX, genY, genZ);」 としているのですが 地下に鉱石が生成された気配が全くありません どこか間違えている・その他に設定が必要 などありましたら教えていただけないでしょうか 長文失礼しました - さくら 2016-04-01 00 10 45 こちらの環境でそのように変更すると、チャンクを読み込もうとしたときにクラッシュしました。鉱石はクリエイティブタブなどから利用できますか?GameRegistry.registerBlockをpreInitで呼び出しているか、GameRegistry.registerWorldGeneratorをinitで呼び出しているかなどを確認してください。WorldGenMinable.generateの次の行に「FMLLog.info("Generated ore. (" + genX + ", " + genY + ", " + genZ + ")");」と入れてログを確認するのも有効かと思います。 - 赤砂蛇凪浜 2016-04-01 06 58 20 返信ありがとうございます。まず報告からなんですが、行けました! 無事に生成されてました。ありがとうございます。で、原因なんですが「GameRegistry.registerWorldGeneratorをinitで呼び出しているかなどを確認してください。」というのが気になったので 上をよく見るとそのような文章があり、「げ」と思ってEclipseを見なおしたところ追加し忘れていました。お騒がせして申し訳ありません。 (int i = 0; i 16; i++) WorldGenMinable(decorationMOD.oreDecoration, 0, 8, Blocks.stone) として無事生成できました。本当にありがとうございました!長文、大変失礼いたしました - さくら 2016-04-02 05 55 25 解決できたようでよかったです。 - 赤砂蛇凪浜 2016-04-02 06 10 29 わかりやすい解説ありがとうございます。アイテムをドロップさせる際にメタデータを指定する為にはどのようにすればよいのでしょうか。よろしくお願いします。 - asajyra 2016-04-02 02 14 53 MOD製作チュートリアル/メタデータを持つブロックの追加でも触れていますが、AluminiumOre内で、Block.damageDropedをオーバーライドするとできます。 - 赤砂蛇凪浜 2016-04-02 06 12 33 WorldGenMinableのコンストラクタの第三引数をバニラの鉱石を参考にしたいのですが、バニラの鉱石のWorldGenMinableってどこにあるんですかね? - 名無しさん 2017-02-08 21 38 00 net.minecraft.world.biome.BiomeDecoratorのコンストラクタにおいて、通常世界の鉱石類のためにWorldGenMinableインスタンスの生成が行われています。(以下、補足。今後のModdingをより便利に行うためにご活用ください。)このような情報はIDEの機能を使うと簡単に調べることができます。今回の場合、コード内の「WorldGenMinable」にカーソルを合わせて「定義へ移動」(EclipseではF3)をすると、Forgeコード内のコンストラクタ記述部に移動でき、WorldGenMinableがコンストラクタを三種類持っていることがわかります。それぞれのコンストラクタ記述部にカーソルを合わせて「呼び出し階層を開く」(EclipseではCtrl+Alt+H)をすると、それぞれがどこから呼び出されているかがわかります。WorldGenMinable(Block p_i45459_1_, int p_i45459_2_)がBiomeDecoratorから呼び出されていることはこうして調べました。 - 赤砂蛇凪浜 2017-02-10 18 39 31 丁寧な回答ありがとうございます。どうにか丁度よい生成量にできました! - 名無しさん 2017-02-10 22 55 50 すみません、鉱石の生成率を鉱脈の大きさを変えるのにはどうすればいいんですか?わかりにくい質問でごめんなさい - 頭の中お花畑 2017-04-13 22 22 48 鉱石の生成率の件は解決しました。鉱脈の大きさを決めるプログラムを教えていただけますでしょうか。何度もすいません - 頭の中お花畑 2017-04-13 23 25 16 「解説」に記載してありますが、WorldGenMinableのコンストラクタの第三引数である"int number"を変更することで大きさを制御できます。これは、生成される可能性のある最大のパターンでの鉱石数を指定しています。 - 赤砂蛇凪浜 2017-04-14 18 34 08 ありがとうございます!早速試してみます! - 頭の中お花畑 2017-04-16 19 26 45
https://w.atwiki.jp/protot/pages/40.html
ストーリーの翻訳>1.MEMORY IN DEATH オープニングムービー チュートリアル開始後 チュートリアル後のムービー 1.MEMORY IN DEATH [死の記憶] オープニングムービー 兵士 All clear. 異常なし。 ALEX MERCER(以下、アレックス):My name is Alex Mercer. I m the reason for all of this. They call me a killer, a monster, a terrorist. I m all of these things. 俺の名はアレックスマーサー。こんなことになった原因だ。 奴らは俺をこう呼ぶ。殺人鬼、化け物、テロリストと。ぜんぶそのとおりだ。 アレックス:Three weeks ago someone released a lethal virus in penn station. I woke up in a morgue. Now I hunt, I kill, I consume, I become. I m going to find out who did this to me. And I m going to make them pay. 3週間前、何者かによって凶悪なウィルスがペンシルバニア駅にばら撒かれた。俺は死体保管所で目覚めた。今や俺は獲物を探し、殺し、食い尽くし、またそれに成りすますことだってできる。俺をこんな化け物に変えたやつを探し出す。落とし前をつけさせてやる。 通信:Ghost Three Nine to ground, do you have target in sight? Over. ゴースト39より地上部隊へ、目標は視認できたか?どうぞ。 兵士:That s negative. いいや。 女性:Help! Help me! 助けて!助けてよ! 兵士:Freeze! Don t move! 止まれ!動くな! 兵士:Walkers! Open up! We re clear, Move up. You, With me. Her too. ウォーカーだ!攻撃開始!クリア。行け!お前、ついてこい。こいつも殺れ。 兵士:That s him! 奴だ! 兵士(アレックス変身中):Where is your commanding officer? 君の指揮官はどこに? 兵士:Sir, He s in Times Square. I ll call for trans... サー、タイムズスクエアにおられます。お呼びしま…… 兵士(アレックス変身中):That won t be necessary. 必要ない。 チュートリアル開始後 アレックス:They took everything from me. They re responsible. And they ll pay! 奴らは俺の全てを奪った。責任は奴らに在る。償いはしてもらうぞ! BLACKWATCH COMMANDER:HQ, send in the Strike squad! HQ、攻撃チームを送り込んでくれ! BLACKWATCH HEADQUARTERS:Strike Team to Zebra grid. Ground Teams set up smoke, over. 攻撃チーム横断歩道へ。地上チーム、スモークを焚け。 目的のBLACKWATCH COMMANDER:(掴んだとき)Shoot him! NOW! Fucking shoot him! こいつを撃て!すぐだ!このくそったれを撃て! アレックス:Time to end this. 終わりだ。 チュートリアル後のムービー 会話の相手:We have less than an hour. What s next? あと一時間も無いな。次はどうする? アレックス:The last person responsible for all of this dies tonight. すべての元凶が今夜、死ぬ。 会話の相手:If we make it out of here. Do you think you re ready? 辿り着けるかね……。覚悟は出来てるか? アレックス:I was made for this. 俺はこの為に造られたのさ。 NEXT>2.UNEXPECTED FAMILY