約 3,447,680 件
https://w.atwiki.jp/pictriver/pages/2.html
PictRiverとは PictRiver公式ページ 2010年6月21日に「アプリ★ゲット」で配信を開始した、写真投稿掲示板アプリである。クリエイターは ＫＹＯ さん。 「パっと撮ってすぐ投稿 実況もできる写真投稿掲示板」がキャッチフレーズである。 現在では、フォルダ内の写真もアップロードする事が可能になった。 主に「ピクリバ」と略称されている。しえのりーと呼ばれてた時もある。 「Appli Of the Year」2010年6月の部ノミネート作品。最終選考まで残ったが惜しくも受賞ならず。 現在では「ドコモマーケット」でも配信をしており、ドコモマーケットの「ｉアプリ・アドバンスアワード2010」での賞を受賞している。 また、月日ごとに起こる極端な過疎にも悩まされている。
https://w.atwiki.jp/casterchronicle/pages/298.html
〔弱点攻撃(じゃくてんこうげき)〕 詠唱 コスト2/水星 瞬動 コスト2以下の敵怪異1体を対象とし、それを破壊する。 開幕☆魔法決闘で登場のコスト2の水星の詠唱。 瞬動とコスト2以下の敵怪異1体を破壊する能力を持つ。 敵ターンに使用できる利点があるものの、破壊対象がコスト2以下と非常に狭い。 コスト2以下の怪異を採用していないデッキも多く存在するため、それらに対しては腐ってしまう点も難しいところ。 また属性は異なるものの〔烈火の反撃〕のように別条件を持ちコストに縛りのない破壊詠唱も存在する。 属性にこだわらなければそれらを採用する方が良いだろう。 カード情報 フレーバー・イラストレーター 沈め。 illust 白野椋 収録 開幕☆魔法決闘 BP01-159 U
https://w.atwiki.jp/sekai_ippukawatta/pages/70.html
Morby centrum駅 レッドライン北端の終着駅。 白色の下地に、無造作に塗られていように見えるピンクや灰色の模様は見る角度によって、白＋ピンク、白＋灰色に見えるように 計算して塗られている。 これは、見える色によって自分がどっちのホームにいるのか分かりやすくする意図があり、さらに直接照明を岩に当てなくても、そういう風な照明効果を狙ってとのこと。 http //www.youtube.com/watch?v=euLO-CzOV5s
https://w.atwiki.jp/deccijapan/pages/24.html
DECCIエンターテインメント
https://w.atwiki.jp/gtsf/pages/36.html
+KART RACE KART RACE ENTRY LIST No ハンドルネーム PSID 車検 1 すてい stymotorsport ○ 2 ラクフォー Racfor ○ 3 ゲオルゲ george800v ○ 4 松永 毛 matsunagake ○ 5 じーさん Mistral_SB01 ○ 6 ぴっし tomatonage ○ 7 エフマート ug0715 ○ 8 MTSP mitosupa77 ○ +NISSAN CHALLENGE NISSAN CHALLENGE ENTRY LIST No ハンドルネーム PSID Machine 車検 1 でろりん derolin30 日産 シルビア spec-R エアロ (S15) 02 ○ 2 GEN R34GEN 日産 シルビア spec-R エアロ (S15) 02 ○ 3 HIDE MperformerHID 日産 シルビア spec-R エアロ (S15) 02 ○ 4 サブ ll___SUB___ll 日産 シルビア spec-R エアロ (S15) 02 ○ 5 CLARK Clark_gt 日産 マーチ 12SR 07 ○ 6 ナベ kovmp423 日産 マーチ 12SR 07 ○ 7 ぶみねこ Bumi-neko 日産 マーチ 12SR 07 ○ 8 マーチ jp-ma_ch 日産 マーチ 12SR 07 ○ 9 modena stefanomodena020 日産 シルビア spec-R エアロ (S15) 02 ○ +SuperGT 300 SuperGT 300 ENTRY LIST No ハンドルネーム PSID Machine 車検 1 オカサン・ジョースター okataku RE雨宮 雨宮アスパラドリンクRX7 04 ○ 2 かろ corolla-axio_74 レクサスIS F RM ‘07 ○ 3 しるびあ sirvia RE雨宮 雨宮アスパラドリンクRX7 04 ○ 4 真紅 Majic-xSINKUx スバル クスコDUNLOPスバルインプレッサ 08 ○ 5 クロウ HDHDODHDF アキュラ アキュラ NSX RM 91 ○ 6 かず kazk11 メルセデス・ベンツ CLK ツーリングカー 00 ○ 7 マツキュー MATSU9right スバル インプレッサ TC ○ 8 かつ katsuminami オートバックス ARTA ガライヤ 08 ○ 9 syun syuntyper1 マツダ RX-7 LMレースカー ○ 10 いっちー ichiro-710 スバル インプレッサ TC ○ 11 ダジャ川レビ児 trueno111 ホンダ CR-Z TC ○ 12 ゆーた YUUTA5027 レクサス ウェッズスポーツIS350 08 ○ 13 ナラ ILOVENARA オペル カリブラ ツーリングカー 94 RESERVE ENTRY LIST No ハンドルネーム PSID Machine 車検 1 ねぎ look_hazard アウディ R8 LMS レースカー (Team PlayStation) 09 ○ +NASCAR NASCAR ENTRY LIST No ハンドルネーム PSID Machine 車検 1 ラルド RARUDO63GT シボレー 2011 Jeff Gordon ＃24 インパラ ○ 2 ふしあな fushianasan12 シボレー 2011 Juan Montoya ＃42 インパラ ○ 3 ころ koro_night フォード 2010 Carl Edwards ＃99 フュージョン ○ 4 TOMO xTOMOx-2JZ-GTE シボレー 2011 Tony Stewart ＃14 インパラ ○ 5 よういち YO1_1963 トヨタ 2011 Denny Hamlin ＃11 カムリ ○ 6 むくむく mkmk_1108_angel フォード 2010 AJ Allmendinger ＃43 フュージョン ○ 7 BZ TEAM_lc_62 トヨタ 2010 Brian Vickers ＃83 カムリ ○ 8 デジ de-zi9 シボレー 2010 Jeff Gordon ＃24 インパラ ○ 9 ミケネコ MI_KE_NE_KO4649 シボレー 2010 Tony Stewart ＃14 インパラ ○ 10 ケーヒン KEIHIN77 シボレー 2010 Dale Earnhardt Jr. ＃88 インパラ ○ 11 ばるす barusu-nsr-0410 トヨタ 2011 Joey Logano ＃20 カムリ ○ 12 アッツー Attsu-31 シボレー 2011 Jimmie Johnson ＃48 インパラ ○ +RALLY CROSS RALLY CROSS ENTRY LIST No ハンドルネーム PSID Machine 車検 1 じゅーご ptx015 三菱 ランサーエボリューション X TC ○ 2 世阿弥+ XanaviNismo スバル インプレッサ セダン WRX STI スペック C タイプ RA RM 05 ○ 3 エムディー Md-kob スバル インプレッサ ラリーカー 03 ○ 4 フジオ fujio4jd フォード フォーカス RS WRC 07 08 ○ 5 れっどぶる男 Z31-11 スバル インプレッサWRC 2008 ○ 6 蠍丸 Sasorimaru スズキ SX4 WRC 08 ○ +DRIFT RACE DRIFT RACE ENTRY LIST No ハンドルネーム PSID Machine 車検 1 エビ a12b56 マツダ RX-7 タイプR (FD, J) 93 ○ 2 雪風 unbroken_arrow 日産 180SX Type X 96 ○ 3 モカ moka_kiyot 日産 スカイライン クーペ 370GT タイプSP 07 ○ 4 ダジャ川レビ児 trueno111 マツダ RX-8 タイプS 07 ○ 5 たけちゃん TEAM_REV_LIMIT53 日産 350Z Roadster (Z33, EU) 03 ○ 6 ポジハメくん zero_1155 日産 シルビア spec-R エアロ (S15) 02 ○ 7 ヴォルフィー wolfgang0913 トヨタ MR2 1600 Gリミテッド スーパーチャージャー 86 ○ 8 マツキュー MATSU9right マツダ アンフィニ RX-7 タイプR (FD) 91 ○ 9 アドバン ADVAN_POTENZA 日産 フェアレディZ Version S（Z33）’07 ○ +SuperGT 500 SuperGT 500 ENTRY LIST No ハンドルネーム PSID Machine 車検 1 おやじ o-ya-ji68 レクサス デンソー ダンロップ サード SC430 08 ○ 2 ミズ mizu312 日産 GT-R GT500 ステルスモデル ○ 3 こんばーす CONVERSE2354 ホンダ エプソン NSX 08 ○ 4 ナッツ君 nattu-871871 日産 ザナヴィ ニスモ GT-R 03 ○ 5 あてぃちゅーど atitude0702 ホンダ カストロール無限 NSX 00 ○ 6 ハミ Hamilton_Senna BMW マクラーレン F1 GTR レースカー 97 ○ 7 えぬよし n-yoshi-umi 日産 カルソニック IMPUL GT-R 08 ○ 8 スカイ Prince32R ホンダ ウイダー HSV-010 (SUPER GT) 11 ○ 9 蒼黒 yukiku ホンダ TAKATA童夢NSX 06 ○ 10 ウルフ worf-1 ホンダ ウイダー HSV-010 (SUPER GT) 11 ○ 11 こう kou0910 日産 カルソニック IMPUL GT-R 08
https://w.atwiki.jp/cmtrpgh/pages/253.html
ダメージに対する耐性に関する項目です。 此処のダメージの珍しさとはダメージ属性の属性分類を参照してください。 ※ただし「(全攻撃)」だけは0として扱わず別枠で出します。 防護系 防護点：ダメージを軽減 攻撃反射：ダメージを反射 攻撃吸収：ダメージを吸収して自分の力に 無敵：ダメージを一切無効化、割合的無効化も含む 攻撃型修正なし、状態異常無効、精神攻撃無効 頑健、真空耐性、水中行動、耐圧 弱点系 ※無敵、攻撃反射、攻撃吸収と弱点の効果範囲がかぶった場合、弱点を優先、防護点は有効 急所：きゅうしょ に あたった！ こうか は ばつぐん だ！ 被ダメージ上昇：受けるダメージが上がってしまう 防護点無視：身体の防護点を無視してダメージ 弱点ダメージ：(特定の属性の効果を受けたとき)追加でダメージを受ける 継続苦痛：特定の状況下でダメージを受ける 行動制限：特定の状況下で行動が阻害
https://w.atwiki.jp/motodic/pages/614.html
エキセントリックチェーンアジャスター（eccentric chain adjuster（英語）） ドライブチェーンアジャスター(未作成)の一種。変則的にギアを噛み合わせた物になっていて、この噛み合せ位置を工具で回して調整する。 一般的なダブルナット式(未作成)よりも簡単に調整でき、また、自動的に左右同時に調整できるため、チェーンラインの狂いを心配する必要が全くない。一部、歯の組み合わせ位置が段階的で微調整できないものもあるが、あまり神経質になる点ではなく、メリットが大きい。 コスト(未作成)面で不利なので数は多くないが標準でついているバイクもあれば、社外品(未作成)のスイングアーム(未作成)に採用されていることもある。 関連語 「ダブルナットアジャスター(未作成)」「チェーン調整(未作成)」「ドライブチェーン(未作成)」「ドライブチェーンアジャスター(未作成)」 2007年04月29日
https://w.atwiki.jp/i_ro/pages/326.html
必要条件 Base Lv 1～140 報酬 Base Exp ┳変動 (下記参照) Job Exp ┛ + ←クリックで目次を開く Introduction Level 1-11 Level 11-25 Level 26-40 Level 41-55 Level 56-70 Level 71-85 Level 86-90 Level 91-99 Level 100以上 Level 100-110 Level 111-120 Level 121-130 Level 131-140 関連項目 Introduction iRO wiki の画像表示 The Leveling Quests are only for experience purposes (except for the 26~40 quests that will supply free Novice Potion ) and are accessible through the Mission Boards in the back of the Eden Group headquarters. These quests give mainly Base EXP and the player may or may not gain Job EXP, depending on the quest. The level restriction applies only when taking the quest. It is possible to take a quest and to get the experience at a lower level after transcending. You can take as many quest as you want by talking to the sign board against the wall and they are all repeatable without any delay unless noted (level 71 and up have specials restrictions). All kinds of Battle Manuals EXP modifier can be used for these quests. VIP accounts gets their bonus to EXP added to these quest rewards. Level 1-11 When you have completed the quest(s), return to Eden Headquarters and speak to Sprakki to get your reward. モンスター 達成数 Base EXP Drops 10 220 Lunatic 10 765 Fabre 10 Chonchon 20 1088 Pupa 10 Peco Peco Egg 20 918 Willow 20 2108 Picky 20 Note A free teleport is offered when accepting the quest directly to monster location. Level 11-25 When you have completed the quest(s), return to Eden Headquarters and speak to Spikle to get your reward. モンスター 達成数 アイテム 個数 Base EXP Hornet 10 - 900 Condor 20 - 1800 Rocker 20 Grasshopper's Leg 10 1400 Wormtail 20 - 2600 Spore 30 - 3900 Thief Bug Egg 20 Chrysalis 10 2900 Muka 20 - 3200 Familiar 20 - 3200 Peco Peco 30 - 5400 Poison Spore 30 Poison Spore 5 5000 Level 26-40 After picking up the quest, you will need to talk to the quest giver NPC× to activate it. When you have completed the quest(s), return to the NPC to get your reward (Except the "Delivery" type, the target will give your reward directly). The level range indicated when picking any of the quests below is disabled - no level requirement. 狩りミッション モンスター 達成数 Base EXP Job EXP 報酬アイテム NPC Poison Spore 10 1000 - Novice Potion 100個 Sponiac(Geffen 103,42) Smokie 10 1000 - Novice Potion 100個 Elder Willow 10 1000 100 Novice Potion 110個 Coco 10 1000 200 Novice Potion 150個 Thara Frog 10 1500 300 Novice Potion 200個 Sponiac - iRO wiki の画像表示 配達ミッション 配達物 配達先 NPC Base EXP 報酬アイテム NPC Insecticide Zoologist ( pay_fild07 82, 89) 1000 Novice Potion 100個 Dieshin Man( moc_ruins 173, 55) Honey Entomologist ( prt_fild03 30, 253) 1000 Novice Potion 100個 Blanket Biologist ( xmas_fild01 92, 57) 1000 Novice Potion 100個 Dieshin Man - iRO wiki の画像表示 Note If you selected Deliver Blankets, talk with Mr. Claus (Aldebaran× 223, 222) to access Lutie Field (xmas_fild01). 収集ミッション アイテム 個数 アイテム2 個数 アイテム3 個数 Base EXP Job EXP 報酬アイテム NPC Skel-Bone 10 - - - - 1000 100 Novice Potion 100個 Dashia( Payon 179, 66) Animal Skin 5 Bear's Footskin 10 - - 1000 - Novice Potion 100個 Worm Peeling 20 Garlet 20 - - 1000 100 Novice Potion 100個 Yoyo Tail 10 Acorn 10 Raccoon Leaf 10 1000 200 Novice Potion 150個 Well-baked Cookie 5 Piece of Cake 1 - - 1000 200 Novice Potion 150個 Mole Whiskers 5 Mole Claw 3 - - 1000 200 Novice Potion 150個 Dashia - iRO wiki の画像表示 Level 41-55 When you have completed the quest(s), return to Eden Headquarters and speak to Rakkis to get your reward. モンスター 達成数 Base EXP Thara Frog 20 9600 Cruiser 15 7200 Kukre 30 15750 Orc Baby 15 7875 Orc Warrior 20 11100 Vadon 15 8550 Megalodon 30 17100 Marse 15 9000 Cornutus 20 12300 Myst 15 9900 Shellfish 30 20250 Marine Sphere 15 10350 Phen 20 13800 Orc Skeleton 15 11025 Zenorc 30 22500 Mummy 15 11250 Level 56-70 When you have completed the quest(s), return to Eden Headquarters and speak to Sparkle to get your reward. 狩り収集ミッション モンスター 達成数 アイテム 個数 Base EXP Job EXP Mummy 30 Rotten Bandage 20 15000 5000 Alligator 30 - 16000 6000 Merman 30 - 17000 8000 Savage 30 - 16000 7000 Sohee 30 - 17000 8000 Marionette 30 Golden Hair 30 17000 7000 Hunter Fly 30 - 16000 6000 収集ミッション アイテム 個数 アイテム2 個数 Base EXP Job EXP Fine Sand 10 Grit 30 18000 8000 Sticky Webfoot 30 - 15000 5000 Huge Leaf 40 - 16000 7000 Maneater Blossom 40 - 17000 7000 Level 71-85 You can take as many quest's location as you want by talking to the sign board against the wall and each location is repeatable. You can get individuals rewards on each sub-quest (kill count) but you won't be able to pick the whole quest(s) back at that specific district until everything is cleared (all quests done inside the same district group). After picking up the quest, you will need to talk to the quest giver NPC located near the district of your choice to activate it. When you have completed the quest(s), return to the NPC to get your reward. 場所 モンスター 達成数 Base EXP NPC Sphinx Dungeon district Requiem 10 各48000 Meidi(Morroc× 35,174) Marduk 10 Pasana 10 Glast Heim district Dark Frame 10 各48000 Ancellia(Geffen 146,132) Evil druid 10 Wraith 10 Raydric Archer 10 Juno× district Grand Peco 20 各48000 Jeanbai(Aldebaran 144,230) Sleeper 20 Goat 20 Harpy 20 Clock Tower district Clock 15 各48000 Lisingshetter(Aldebaran 105,116) Punk 15 Rideword 15 Island district Mi Gao 15 各48000 Funfi(Alberta× 215,111) Uzhas 15 Miyabi Doll 15 Meidi - iRO wiki の画像表示 Ancellia - iRO wiki の画像表示 Jeanbai - iRO wiki の画像表示 Lisingshetter - iRO wiki の画像表示 Funfi - iRO wiki の画像表示 Note Island district can be problematic (2 out of 3 monsters require access quest or dungeon teleport scrolls to get to them). Level 86-90 このクエスト若しくはインスタンスは反復可能但し３時間のクールタイムがある You can take as many quest as you want by talking to the sign board on second floor and they are all individually repeatables after a 3 hours cooldown. When you have completed the quest(s), return to Eden Headquarters and speak to the same sign board and select the mission you have completed to get your reward. モンスター 達成数 Base EXP Dragon Tail 30 76000 Spring Rabbit 30 82000 Pest 30 82000 Bathory 30 72000 Alarm 30 76000 Baba Yaga 30 72000 Yao Jun 30 117000 Firelock Soldier 30 82000 Zipper Bear 30 120000 Ground Petite 30 72000 Rafflesia 30 72000 Venomous 30 72000 Pitman 30 86000 Yellow Novus 30 80000 Level 91-99 このクエスト若しくはインスタンスは反復可能但し３時間のクールタイムがある You can take as many quest as you want by talking to the sign board on second floor and they are all individually repeatable after a 3 hours cooldown. When you have completed the quest(s), return to Eden Headquarters and speak to the same sign board and select the mission you have completed to get your reward. モンスター 達成数 Base EXP Job EXP Solider 30 90000 311000 Freezer 30 94000 Heater 30 114000 Injustice 30 96000 Rybio 30 96000 Dark Priest 30 146000 Stapo 30 96000 Roween 30 102000 Siroma 30 110000 Shinobi 30 102000 Evil Nymph 30 108000 Deviruchi 30 110000 Mineral 30 138000 Kaho 30 118000 Neraid 30 96000 Disguise 30 134000 Level 100以上 このクエスト若しくはインスタンスは反復可能但し１時間のクールタイムがある Level 100 and above Eden Quests were introduced to iRO with 13th November 2013 maintenance. The NPCs offering these quests are situated behind the blue access door located in the NE corner of Eden floor 1. Upon entering take the left portal to enter the NPC room. Level 100-110 1. Speak to Gelkah ( moc_para01 (*1)37,95) located inside the first room on the left behind the blue access door located in the north east corner of Eden Group. 2. She will ask you to bring her 50 Brigan . Once you bring the items back she will reward you with a Courtesy Ticket . 3. Speak to Rohtert (moc_para01 41,95). 4. Gelkhah will then offer a number of hunting quests. You can take as many quest as you want by talking to Gelkah and they are all individually repeatable after a 1 hour cooldown. 5. Return to Gelkhah and select the completed quest in order to receive the reward. 場所 モンスター 達成数 Base EXP Job EXP 報酬アイテム Glast Heim Carat 30 154000 148000 Courtesy Ticket Arclouse 22 154000 148000 Anolian 30 198400 186500 Sting 30 198400 186500 Majorous 30 252000 252000 Ash Vacuum Pinguicula 30 154000 148000 Luciola Vespa 30 198400 186500 Ice Dungeon Desert Wolf 30 154000 148000 Ice Titan 30 198400 186500 Snowier 30 154000 148000 Magma Dungeon Nightmare Terror 30 154000 148000 Deleter (Sky) 30 154000 148000 その他地域 Loli Ruri 30 198400 186500 Medusa 30 154000 148000 Anubis 20 198400 186500 10, 20 or 30 Courtesy Tickets can be exchanged for EXP by listening to a story. 10 Courtesy Tickets gives a reward of 80000 Base EXP and 168000 Job EXP or 100000 Base EXP and 148000 Job EXP. 20 Courtesy Tickets gives a reward of 300000 Base EXP and 240000 Job EXP. 30 Courtesy Tickets gives a reward of 500000 Base EXP and 430000 Job EXP. 1 or 10 Courtesy Ticket(s) can be exchanged for item rewards. Possible rewards for 1 Courtesy Ticket include 5 or 10 Condensed White Potion s. Possible rewards for 10 Courtesy Tickets include 1 Old Blue Box , 100 Condensed White Potions or 1 Old Card Album . Level 111-120 1. Speak to Mingmin (moc_para01 17,95) located inside the first room on the left behind the blue access door located in the north east corner of Eden Group. 2. You will need to speak with her several times and agree to sign a contract before the hunting quests can be unlocked. 3. Mingmin will then offer a number of hunting quests. You can take as many quest as you want and they are all individually repeatable after a 1 hour cooldown. 4. Return to Mingmin and select the completed quest in order to receive the reward. 場所 / 依頼 モンスターまたはアイテム 達成数 Base EXP Job EXP Glast Heim Raydric 30 300000 200000 Khalitzburg 30 300000 200000 Wanderer 30 300000 200000 Thanatos Tower Ancient Mimic 30 ?? ?? Death Word 30 400000 300000 Owl Baron 20 ?? ?? ※ Bloody Page 20 400000 300000 Ash Vacuum Dark Pinguicula 30 280000 180000 Nepenthes 30 280000 180000 Naga 30 280000 180000 Centipede Larva 20 280000 180000 Cornus 30 280000 180000 ※ Mystic Horn 20 280000 180000 Mingmin の個人的な依頼 Pyramid Ancient Mummy 30 ?? ?? Geffenia Survey Incubus 10 ?? ?? Succubus 10 Violy 10 Juperos Survey Venatu (Green) 30 ?? ?? Venatu (Orange) 30 Venatu (Blue) 30 Juperos ※ Fragment 10 300000 200000 ※ Rusty Screw 20 訳注 ： ※の付いた物はアイテム収集 Level 121-130 1. Speak to Aigu (moc_para01 17,77) located inside the first room on the left behind the blue access door located in the north east corner of Eden Group. 2. Speak to Melody Jack (moc_para01 44,81). Abyss lake search - Abyss Lake Dungeon 3 (101,139) 場所 モンスター 達成数 Base EXP Job EXP Thanatos Tower Baroness of Retribution 30 ?? ?? Lady Solace 30 ?? ?? Mistress of Shelter 30 ?? ?? Dame of Sentinel 30 ?? ?? Rachel Shrine Vanberk 20 500000 200000 Isilla 20 Hodremlin 30 500000 200000 Agav 20 500000 200000 Echio 20 Nameless Island Ragged Zombie 30 500000 200000 Banshee 30 500000 200000 Abyss Lake Dungeon Ferus (*2) 30 ?? ?? Bewler (*3) 30 Blue Acidus 30 ?? ?? Gold Acidus 30 Level 131-140 1. Speak to Ragi (moc_para01 41,76) located inside the first room on the left behind the blue access door located in the north east corner of Eden Group. 1. incarnation of morroc 30 ea - shadow, angel ghost - 800000 base exp + 200000 job exp 2. all 4 scaraba - uni, horn, antler, rake - 30 ea - 800000 base + 200000 job 関連項目 - Eden Group Quests Eden Group Quests Equipment Quests ・ Leveling Quests ・ Crystal Synthesis×Eden Merit Badge ・ Weekly Turn-In Events× ・ VIP Turn-Ins× - Leveling Leveling Levels Experience ・ Base Level (Base EXP Chart ・ Base EXP Quests×) ・ Job Level (Job EXP Chart ・ Job EXP Quests×) ・ パーティシステム ・ Guild Level× ・ Homunculus Level (S-Type×) Leveling Methods Bounty Board Quests • Eden Group Leveling Quests · 狩場情報 · EXP Quest Table ・ Weekly Turn-In Events× (Gramps) Categories Quest Window Quests | Job Experience Reward | Base Experience Reward | Repeatable Quests | Eden Group
https://w.atwiki.jp/th_izime/pages/1315.html
39 名前が無い程度の能力 [ｓａｇｅ] 2012/02/05(日) 21 33 47 ID eHsrMus60 魔理沙「マスタースパーク」 さとり「マカラカーン」 こいし「ミラーコート」 魔理沙「」 魔理沙やレミリアみたいな超火力一発技なタイプって一属性だけ完全ガードな防具とか カウンター攻撃に凄く弱そうな気がする 40 名前が無い程度の能力 [sage] 2012/02/05(日) 22 37 01 ID fgk.jZJ60 レミは全ステ高いけど弱点多いタイプだろ 41 名前が無い程度の能力 [sage] 2012/02/06(月) 12 34 03 ID seWpawRM0 プレスターンで勝負を挑まれて涙目になるタイプ 42 名前が無い程度の能力 [sage] 2012/02/06(月) 13 54 59 ID UW04ow.20 魔理沙：呪殺弱点 レミリア：破魔弱点 43 名前が無い程度の能力 [sage] 2012/02/06(月) 18 18 05 ID 7rcoq9sU0 マカジャマオンで詰むアリスとパチュリー。 テトラカーンで手も足も出なくなる萃香と勇儀。 44 名前が無い程度の能力 [sage] 2012/02/06(月) 23 00 14 ID K.9sua3U0 全ての属性に耐性があるが無効が一つもない霊夢(もちろん破魔も効く) ターンごとに耐性が入れ替わりまくる紫(曖昧) 耐性が正体不明なぬえ(見えない。全部「？」) 耐性が弱点か無効かしかない四季様(白黒はっきりしてる) 46 名前が無い程度の能力 [sage] 2012/02/07(火) 00 45 06 ID 6vXbCytw0 ≫43 そういやマカジャマオン→死神の点呼で即死コンボ決めれたような・・・ 47 名前が無い程度の能力 [sage] 2012/02/07(火) 01 10 06 ID GT/ATbeA0 パチュリー「獣の眼光！マカカジャ！マカカジャ！獣の眼光！マカカジャ！獣の眼光！獣の眼光！マカカジャ！マカカジャ！」 48 名前が無い程度の能力 [sage] 2012/02/07(火) 01 21 44 ID 9fF2V1ss0 →ロイヤルフレア モト劇場か…。 -- 名無しさん (2012-09-17 06 09 03) 名前 コメント
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01July/2012 最近の、DAG研究の動向と方向性 基本的に、あまり進んでいない。2006年のうちらの論文および2010年のNewton ACの論文によるそれぞれの膜においてのシグナルのcompartmentalizeが、cell biologyのDAGに関する新しい発見である。これは、imaging技術の発展なしにしてはわからなかったことである。 24/Mar/2011 I found this paper with the search with the word, SMase and diacylglycerol. Methylmercury-induced toxicity is mediated by enhanced intracellular calcium through activation of phosphatidylcholine-specific phospholipase C D609 06/Dec/2010 These are the papers related to that DAG is cruical for membrane funsion. 1. Biochemistry. 1989 May 2;28(9) 3703-9. Physiological levels of diacylglycerols in phospholipid membranes induce membrane fusion and stabilize inverted phases. Siegel DP, Banschbach J, Alford D, Ellens H, Lis LJ, Quinn PJ, Yeagle PL, Bentz J. Procter and Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45239-8707. 2.J Cell Biol. 2004 Dec 20;167(6) 1087-98. Interdependent assembly of specific regulatory lipids and membrane fusion proteins into the vertex ring domain of docked vacuoles. Fratti RA, Jun Y, Merz AJ, Margolis N, Wickner W. Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA. In this paper, they say DAG has inhibitory effect on vesicle fusion. 3.J Biol Chem. 2004 Dec 17;279(51) 53186-95. Epub 2004 Oct 12. Diacylglycerol and its formation by phospholipase C regulate Rab- and SNARE-dependent yeast vacuole fusion. Jun Y, Fratti RA, Wickner W. Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755-3844, USA. Paper In this paper, they say that DAG is masked by C1B domain, vacuola fusion is inhibited. 4. Biochem Biophys Res Commun. 2010 Apr 9;394(3) 733-6. Epub 2010 Mar 15. A novel complete reconstitution system for membrane integration of the simplest membrane protein. Nishiyama K, Maeda M, Abe M, Kanamori T, Shimamoto K, Kusumoto S, Ueda T, Tokuda H. Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. nishiyam@iwate-u.ac.jp paper 5.Biochim Biophys Acta. 2010 Jan;1798(1) 59-64. Epub 2009 Nov 3. End-products diacylglycerol and ceramide modulate membrane fusion induced by a phospholipase C/sphingomyelinase from Pseudomonas aeruginosa. Ibarguren M, Bomans PH, Frederik PM, Stonehouse M, Vasil AI, Vasil ML, Alonso A, Goñi FM. Unidad de Biofísica (Centro Mixto CSIS-UPV/EHU), y Departamento de Bioquímica, Universidad del País Vasco, Apto. 644, 48080 Bilbao, Spain. Paper 6.Biophys J. 2009 May 6;96(9) 3638-47. A comparison of the membrane binding properties of C1B domains of PKCgamma, PKCdelta, and PKCepsilon. Sánchez-Bautista S, Corbalán-García S, Pérez-Lara A, Gómez-Fernández JC. Departamento de Bioquímica y Biología Molecular A, Facultad de Veterinaria, Universidad de Murcia, E-30080-Murcia, Spain. Paper 7.Biochim Biophys Acta. 2009 Mar;1788(3) 701-7. Epub 2008 Dec 3. Calcium inhibits diacylglycerol uptake by serum albumin. Ahyayauch H, Arana G, Sot J, Alonso A, Goñi FM. Unidad de Biofísica (Centro Mixto CSIC-UPV/EHU), and Departamento de Bioquímica, Universidad del País Vasco, Aptdo. 644, 48080 Bilbao, Spain. Paper 8. Progress in Lipid Research Volume 38, Issue 1, January 1999, Pages 1-48 Structure and functional properties of diacylglycerols in membranes Félix M. Goñi* and Alicia Alonso Grupo Biomembranas (Unidad Asociada al CSIC), Departamento de Bioquímica, Universidad del País Vasco, Aptdo. 644, 48080 Bilbao, Spain Paper 20Dec2010 About the experiment for biological significance of outer DAG. Previously I did experiment about the difference in cell growth between the cells with or without DAG probe. At the time, it seems that the flowting cells exist more in the cells incubated with DAG probe. This means that cell growth is suppressed with DAG probe. But MDCK cells s growth is very fast. I am gonna reduce the % of serum and make growth rate slow down. 14Dec2010 I need to finish this paper now around. But there are several things I have to do... 1. see if DAG comes out to the outer plasma membrane in response to ATP. 2. biological significance of outer DAG. The day after tomorrow, I gonna do the following expreiments. 1. Time course of outer DAG production in response to ATP in MDCK cells. 2. inhibitor of R59949 and U73122 at the time point of 10 min 3. mGluR expressed MDCK cells 1. SMnaseBcPLC, SMnase, ATP, ctrl. 1. ctrl(no D609),ctrl(D609), 1min(D609,ATP) 4min, 7min,10min. 2. ctrl(no D609), ctrl(D609), ctrl(D609ATP)D609ATPU73122, D609ATPR59949, biological significance 1. cell growth 2. incorporation of LDL or something. 24/Nov/3010 I am gonna do the following experiments. 1) 1. Incubate with 1 microM ATP and 10 U SMase and 12.5 unit/ml Bc-PLC for 4 min and 10 min and 10 min respectively. Do nothing about ctrl. 2. Fix cells with 3 % PFA for 15 min. 3. Add NH4Cl for 5 min. 2) Preincubation with D609 for 5 hour. 1. Preincubation of the cells with 15micro/L U73122 for 10 min shortly before starting experiments. 2. ATP stimulation and after 10 min, incubate with EFYP-C1AB for 2 min. 3. Fix cells with 3 % PFA for 15 min. 4. Add NH4Cl for 5 min. 22/Nov/2010 Transfection of DsRED2 and EYFP-C1AB into MDCK cells. What I want to do is that examination of DAG in stady state in ctrl SMase, Bc-PLC and ATP treated MDCK cell. 28/Oct/2010 I am gonna do the experiment for the paper. 1. incubation with D609 for 0, 1, 3, 6 and 12 hrs. 2. ATP stimulation after incubation with D609 for 6 hrs. 3. the effect of SMase, ATP and BcPLC on the inner DAG. 4. the comparison between Bc-PLC and ctrl. 12 33 12 hour incubation finish 17 06 6 hour incubation finish 12 12 1 hour incubation finish 14 12 3 hour incubation finish. 15/Oct/2010 The things I am gonna do from now is as follows. 1. At first, it is very weird that DAG flip should occured in stady state upon addition of SMase or incubation with ISP1. So,I am gonna observe DAG in inner leaflet of plasma membrane. RFP and YFP-C1AB are expressed in MDCK cells. Fix this cell. If there are some making a difference in fluorescence between RFP and YFP, YFP-C1AB can detect inner DAG. After identifing this, I am gonna do SMase addition to MDCK cells. I gonna compare the fluorescence pattern with or without SMase. 14/Oct/2010 Today I am gonna do the experiment of staining of lymphocyte with DAG probe. The way to do is something like that. MDCK, SMS-+ and Jurkat cells. (Actually I picked up 5×105 lympho cells 50microl) I want to difference in staing between MDCK and other lympho cells. 1. trypsinize 2. rip off the cells. 3. spin down 4. replace medium with HBSS. 5. incubate with DG probe for 2 min 6. fixed with 3%PFA And then, I want to see the staining pattern of Jurkat cells which have aggrigation. 1. pich up several Jurkat cells on the glass base dish. 2. replace the medium with HBSS carefully in the glass base dish. 3. 2 min incubation with DG probe 4. wash with HBSS 3 times carefully 5. fixed with 3% PFA. 07/Oct/2010 今日のDG I examined the effect of D609 on DAG on the outer leaflet of plasma membrane several days ago. Previously I thought that DAG is gonna dissapear after 6hour. However I found out that DAG dissapear more quickly within 3 hour. Basically within 1 hour, almost of DAG dissapears.So I am gonna do the following experiment next. 1. Incubate with D609 for 3 hour. At this time, I believe that internalization of recepters does not occur. So I can examine DAG flop that is drived from DAG producing in response to ATP stimulation. 2. 100microM ATP stimulation and stain the outer leaflet of DAG at 0,2,4,7,10 min. 17/Sep/2010 I did following experiments. MDCK cells were incubated with 50microg/ml D609 for 6 hour. ripped off the cells with trypsine. spread MDCK cells 24 well dish with D609. After 1 hrs, wash out the flouting cells 3 times, fixed and counted the number of cells. In control cells without D609, cells attached the bottom of dish well. However in the presence of D609, cells hardly attached the dish. 07/Sep/2010 The experiments below have been very much done. So what is the next step? The paper s theme is something involved in DAG flip-flop. So it is better focusing on DAG flip-flop. What I gonna do is as follow. 1. I am gonna check that there is no difference in cytosolic DAG production in response to ATP between with or without D609. I afraid of Receptors internalization during incubation with D609 as previously shown in some papers. If I can, I want to fix these cells because I can count cell numbers easily. 25/Aug/2010 MDCK was incubated with some drug as follows. 1. 10% LPDS 24 hrs 2. ISP-1 50 nM 24 hrs 3. FB1 40 microM 24 hrs 4. D609 50 mircog/ml 24 hrs 5. U73122 500 nM 24 hrs. 6. D609 50 microg/ml 24 hrs and U73122 500 nM 24 hrs. 7. D609 50 microg/ml 6 hrs 8. ctrl 19/Aug/2010 MDCK cells were incubated with 200 microM D609 for 12hour. But cells died perfectly. So I can not use long incubation with 200 microM D609. I am gonna try 6hour. 18/Aug/2010 The results of yesterday experiment. In fact, ISP-1 treated cells give annexin V fluorescence a little bit without Bc-PLC. After incubation with 1.25units/ml Bc-PLC, in the case of 200 nM ISP-1, fluoresecence of annexin V increases little by little. Same situation gonna happen in the case of 50 nM ISP-1. But labelling with annexin V is very weaker than 200 nM. This is the level I can ignore. So I put 50nM IPS-1 treated cells in my paper. 17/Aug/2010 Today I gonna do the following experiments. 13/Aut/2010, I did 12.5 unit/ml BcPLC application experiment. I want to confirm that ISP-1 treated cells do not have cell damages during incubation with 12.5 unit/ml Bc-PLC using anexin V. And I want to confirm whethere DAG produced by Bc-PLC is same between tISP-1 treated cells and normal cells. 13/Aug/2010 I did several important expriments. 1. whether ZS2, ZS2/SMS1 and ZS2/SMS2 have DAG on the outer leaflet of PM. Answer Every cells was stained with DAG probe at the same level. This shows that PC-PLC activity is different from SMS1 and SMS2. 2. whether D609 diminishes the outer DAG amount in MDCK cells. Answer at 6hour DAG diminished however 24 hour incubation recovered the DAG? What happen?? Anyhow I am gonna take reproducibility. 3. Glutamte applies MDCK cells expressing mGluR5. 20 microM glutamate was added to this MDCK cells. However there is no difference between not expressed cells and expressed cells. This data shows that DAG does not flop to the outer leaflet of the PM. 4. 1.25 unit/ml Bc-PLC was added to the MDCK cells treated with 50 and 200 nM ISP-1. Flip occured! 10/Aug/2010 Here is most important paper now for me. D609 D609-1 D609-2 In the experiment of 10/Aug/2010, we could not see difference in fluorescence between D609 24hour incubated MDCK cells and intact MDCK cell. So I am going to do this reproducibility again. And then I am gonna start ISP1 incubation again. 06/Aug/2010 I am gonna do ISP-1 treatment expeiment. I am gonna divide MDCK cells, which exposed 200nM and 50nM ISP-1 for observation and biochemistry expeiments. So I gonna prepare six 3.5mm well dishs for each. And I gonna do the experiment in which I want to see the effect of D609 on the receptor signalling. Because it is known that D609 causes the internalization of receptors from plasma membrane. So I prepare MDCK cells expressing inner DAG probe in three dishes. 03/Aug/2010 I got mGluR-RFP from Matsuura-kun. I can express this DNA whatever cells I want. I am gonna express this DNA CHO cells and MDCK cells. Today s result. I looked through the samples I prepared yesterday. Now I found out that DAG exists in static state. Based on this result, I need to plan the expriments. Basically, this expreiment follows the experiment of CHO cells. 02/Aug/2010 I am gonna do the experiment as follows. 1. no probe, probe only, ATP, ATP+R59949, ATP+U73122, PC-PLC. 2. MDCK cell growth with or without DAG probe. 01/Aug/2010 I am doing the experiments in which 10 microM R59949 incubation is done upon 100 microM ATP addition. I expect that more DAG comes to outer leaflet of PM than without R59949. 10 min R59949 incubation and then ATP stimulation containing R59949. 2 min incubation with DAG probe.