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https://w.atwiki.jp/metalmetabo/pages/34.html
- visitors In vivo analysis of intracellular amino acid labelings by GC/MS. C Wittmann, M Hans, E Heinzle - Analytical biochemistry, 2002 - mendeley.com ... TBDMS derivatization of amino acids is especially useful in metabolic network analysis, because M-57 fragments containing the entire carbon skeleton of the analyte can be observed by GC/MS with high signal intensities 8. The potential of the method is exemplified for ... Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose nih.gov [HTML]P Kiefer, E Heinzle, O Zelder, C … - Applied and …, 2004 - Am Soc Microbiol ... A comprehensive approach to 13 C tracer studies, labeling measurements by gas chromatography-mass spectrometry, metabolite balancing, and isotopomer modeling, was applied for comparative metabolic network analysis of lysine-producing Corynebacterium glutamicum ... It is all about metabolic fluxes nih.gov [HTML]J Nielsen - Journal of bacteriology, 2003 - Am Soc Microbiol http //scholar.google.com/scholar?q=related xAvLLrdbbNYJ scholar.google.com/ hl=ja as_sdt=2000 ... I. Experimental observations. Biotechnol. Bioeng. 55 305-316.[CrossRef] 21; Thykaer, J., B. Christensen, and J. Nielsen. 2002. Metabolic network analysis of an adipoyl-7-ADCA producing strain of Penicillium chrysogenum elucidation of adipate degradation.Metab. Eng. ... Using topology of the metabolic network to predict viability of mutant strains biomedcentral.com [PDF]Z Wunderlich, L Mirny - Genome Biology, 2005 - biomedcentral.com http //scholar.google.com/scholar?q=related sTavonuje7wJ scholar.google.com/ hl=ja as_sdt=2000 ... Ph.D. Thesis. University of Leeds; 1994. 14. Papp B, Pal C, Hurst LD Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast. Nature 2004, 429(6992) 661-664. 15. Jeong H, Tombor B, Albert R, Oltvai ZN, Barabasi AL The large-scale ... Novel proteins, putative membrane transporters, and an integrated metabolic network are revealed by quantitative proteomic analysis of Arabidopsis cell … plantphysiol.org [HTML]H Eubel, EH Meyer, NL Taylor, JD Bussell, N O … - Plant …, 2008 - Am Soc Plant Biol http //scholar.google.com/scholar?q=related aCgbR-dAtyMJ scholar.google.com/ hl=ja as_sdt=2000 ... This is aided by a metabolic network analysis that reveals a tight integration of functions and highlights specific metabolite nodes that most probably represent entry and exit metabolites that could require transport across the peroxisomal membrane. ... High-throughput phenomics experimental methods for mapping fluxomes bjmu.cn [PDF]U Sauer - Current opinion in Biotechnology, 2004 - Elsevier http //scholar.google.com/scholar?q=related XvhSxXXwbn4J scholar.google.com/ hl=ja as_sdt=2000 ... From its early days when material fluxes were balanced within assumed reaction networks [7.], metabolic network analysis [6. and 8.] has matured to actually identify the topology of active reactions and pathways and to quantify the molecular flux through them on a variety of ... Use of genome-scale microbial models for metabolic engineering KR Patil, M Åkesson, J Nielsen - Current opinion in biotechnology, 2004 - Elsevier http //scholar.google.com/scholar?q=related oAOz5SBTVHAJ scholar.google.com/ hl=ja as_sdt=2000 ... incorporate genome-scale biological data. Genome-scale stoichiometric models of microorganisms represent a first step in this direction. FBA, flux balance analysis; MFA, metabolic flux analysis; MNA, metabolic network analysis. ... Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression nih.gov [HTML]AK Gombert, M Moreira dos Santos, B … - Journal of …, 2001 - Am Soc Microbiol http //scholar.google.com/scholar?q=related R5V_AodxCrwJ scholar.google.com/ hl=ja as_sdt=2000 ... flux estimates. The use of 13 C-labeled substrates enables both identification of the metabolic network structure and quantification of the metabolic fluxes and is therefore referred to as metabolic network analysis (5). This kind ... Metabolic flux analysis of Escherichia coli K12 grown on 13C-labeled acetate and glucose using GC-MS and powerful flux calculation method freelogy.org [PDF]J Zhao, K Shimizu - Journal of biotechnology, 2003 - Elsevier http //scholar.google.com/scholar?q=related w1m-5IKYKpsJ scholar.google.com/ hl=ja as_sdt=2000 Structural and functional analysis of cellular networks with CellNetAnalyzer biomedcentral.com [HTML]S Klamt, J Saez-Rodriguez, ED Gilles - BMC Systems Biology, 2007 - biomedcentral.com http //scholar.google.com/scholar?q=related bzwlVku0FL8J scholar.google.com/ hl=ja as_sdt=2000 ... CNA extends its predecessor FluxAnalyzer, originally developed for metabolic network analysis[7], by new methods for signalling and regulatory networks, ie for networks where signal flows are dominating (in contrast to mass flows in metabolic networks). ...
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- visitors Metabolic network analysis B Christensen, J Nielsen - Bioanalysis and biosensors for bioprocess …, 2000 - Springer http //scholar.google.com/scholar?q=related PiGOMNyMhjYJ scholar.google.com/ hl=ja as_sdt=2000 Metabolic network analysis is a tool for investigating the features that identify the topology of a metabolic network and the relative activities of its individual branches. The pillars of metabolic network analysis are mathematical modeling, allowing for a quantitative analysis, ... Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast B Papp, C Pál, LD Hurst - Nature, 2004 - nature.com http //scholar.google.com/scholar?q=related 0sM60oXJ2jQJ scholar.google.com/ hl=ja as_sdt=2000 Under laboratory conditions 80% of yeast genes seem not to be essential for viability 1 . This raises the question of what the mechanistic basis for dispensability is, and whether it is the result of selection for buffering or an incidental side product. Here we analyse these issues ... Genealogy profiling through strain improvement by using metabolic network analysis metabolic flux genealogy of several generations of lysine-producing … nih.gov [HTML]C Wittmann, E Heinzle - Applied and environmental microbiology, 2002 - Am Soc Microbiol http //scholar.google.com/scholar?q=related 49I4bRT2XYIJ scholar.google.com/ hl=ja as_sdt=2000 A comprehensive approach of metabolite balancing, 13 C tracer studies, gas chromatography-mass spectrometry, matrix-assisted laser desorption ionization-time of flight mass spectrometry, and isotopomer modeling was applied for comparative metabolic ... Metabolic network analysis of Penicillium chrysogenum using 13C-labeled glucose B Christensen, J Nielsen - Biotechnology and …, 2000 - interscience.wiley.com http //scholar.google.com/scholar?q=related 9XjgWNCKNqMJ scholar.google.com/ hl=ja as_sdt=2000 To provide a rational approach for directed genetic alter- ations in microorganisms, often referred to as metabolic engineering (Bailey, 1991; Stephanopoulos, 1999; Yarmush and Berthiaume, 1997), a detailed map of the fluxes in the central metabolism is an essential ... Metabolic network analysis of Bacillus clausii on minimal and semirich medium using 13C-labeled glucose T Christiansen, B Christensen, J Nielsen - Metabolic engineering, 2002 - Elsevier http //scholar.google.com/scholar?q=related GeX9jXLzh3EJ scholar.google.com/ hl=ja as_sdt=2000 Using 13 C-labeled glucose fed to the facultative alkalophilic Bacillus clausii producing the alkaline serine protease Savinase, the intracellular fluxes were quantified in continuous cultivation and in batch cultivation on a minimal medium. The flux through the pentose phosphate ... Genome-scale metabolic network analysis of the opportunistic pathogen Pseudomonas aeruginosa PAO1 nih.gov [HTML]MA Oberhardt, J Puchalka, KE Fryer, VAP … - Journal of …, 2008 - Am Soc Microbiol http //scholar.google.com/scholar?q=related isrhQUVHkSMJ scholar.google.com/ hl=ja as_sdt=2000 Pseudomonas aeruginosa is a major life-threatening opportunistic pathogen that commonly infects immunocompromised patients. This bacterium owes its success as a pathogen largely to its metabolic versatility and flexibility. A thorough understanding of P. aeruginosa s ... A metabolic network stoichiometry analysis of microbial growth and product formation WM Van Gulik, JJ Heijnen - Biotechnology and …, 1995 - interscience.wiley.com http //scholar.google.com/scholar?q=related p9UTGWFLJJUJ scholar.google.com/ hl=ja as_sdt=2000 ... Other work on metabolic network analysis using linear op- timization techniques has been performed on hybridoma cell Esehenchiu coli metabolism,3 .32 and acetate overflow in E. c01i.""~ Following the approach to solve underdetermined metabolic networks by conducting ... Metabolic network analysis of lysine producing Corynebacterium glutamicum at a miniaturized scale C Wittmann, HM Kim, E Heinzle - Biotechnology and …, 2004 - Wiley Online Library http //scholar.google.com/scholar?q=related B7ezo5R5OxIJ scholar.google.com/ hl=ja as_sdt=2000 Abstract We present a straightforward approach com- prising 13C tracer experiments at 200-AL volume in 96-well microtiter plates with on-line measurement of dissolved oxygen for quantitative high-throughput metabolic net- work analysis at a miniaturized scale. This ... Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast biomedcentral.com [HTML]LM Blank, L Kuepfer, U Sauer - Genome Biology, 2005 - biomedcentral.com http //scholar.google.com/scholar?q=related TodhI8VNW54J scholar.google.com/ hl=ja as_sdt=2000
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■Spam Mail Killerの入手方 製作者の土肥 英明氏のウェブサイトeimei's laboratory Vector 窓の杜 （どこからダウンロードしても同じです） ■■■項目をクリックするとジャンプします■■■ 豆知識 Tips リンク■2chスレ お役立ちリンク 適当に編集してください。 項目が増えてきたら見出し毎に別ページに入れていきませう。 おすすめの禁止リスト＠複数要素 豆知識 ■マッチング（判定）のプライオリティ（優先度）について 設定した条件には以下のような優先順序があります。 SMK作者の土肥さんより、下記情報をいただきました。 Ver.2.16～2.21における各条件の優先順位は以下の通りです。 1. 許可リスト（ヘッダー処理系のみ） 2. 禁止リスト＠複数要素（ヘッダー処理系のみ） 3. 禁止リスト＠正規表現（ヘッダー処理系のみ） 4. テンプレート（ヘッダー処理系のみ） 5. 禁止リスト＠差出人、受取人 6. 宛先（To ）に指定アドレスが含まれない 7. 日付（Date ）が無いか不正な書式 8. 許可リストに登録されていない 9. 禁止リスト＠件名、ヘッダー 10. 件名に「未承諾広告※」および類似語を含む 11. メールサイズで制限 12. 文字（言語）コード指定で制限（ヘッダー処理系のみ） 13. 許可リスト（本文処理系のみ） ※(1) 14. 禁止リスト＠複数要素（本文処理系のみ） 15. 禁止リスト＠正規表現（本文処理系のみ） 16. テンプレート（本文処理系のみ） 17. 文字（言語）コード指定で制限（本文処理系のみ） 18. 禁止リスト＠本文 19. 件名＆本文の２バイト文字が計４個以下 20. 添付ファイルの拡張子で制限 21. 本文と同時に受信したヘッダーを対象に3.および9.を再チェック ※(2) 22. html部分をHTML→TEXT変換して13.～20.を再チェック ※(3) ※(1) 許可リストに本文関連の指定（## body 指定）がある場合、 1.に該当せず、2.～12.に該当して削除対象と判断されても、 引き続き本文を受信して13.の確認を行なう。 結果、13.に該当すれば当該メールは2.～12.に関わらず、 削除対象外であると判断する。 ※(2) POPFileと連携させる場合、本文と同時に受信したヘッダーでないと POPFileの分類情報が確認できないので、本文受信後の件名やヘッダーの 再チェックが必要となる。 ※(3) [削除設定→html形式の本文チェックに HtoX を使用]がONで、 本文に有効なhtmlが含まれている場合のみ再チェックする。 データ受信量と負荷が大きくなる本文処理は意識的に後回しにされているので、可能な限りはヘッダで処理するように心がけた方が動作も軽く処理も速い。 また、メルマガや友人などのアドレスを許可リストに積極的に入れておくようにすれば、２番以下のマッチングを一切行うことなくスルー判定されるため非常に効率よく処理できるようになる。 Tips ■その１ プロバイダ等でウイルスメールチェックのサービスを利用しており、それによってウイルスメールを受信することがほとんどないのであれば、テンプレートの０００～０２９のチェックをOFFにすることで全体の処理速度を向上させることができる。 ■その２ テンプレートとかたくさん使いながら「禁止リスト＠差出人」などのいわゆる（複数要素に対する）単一要素でもたくさん登録している人向けの小技。 これらの条件を「禁止リスト＠複数要素」に一括登録すると、プライオリティが上がるため処理が早くなる。 例えば「禁止リスト＠差出人」に次のように登録してあれば aaa＠xyz.com bbb＠xxx.co.jp ＠ooo.com ＠*adult*.* これを複数要素に置き換えると・・・ ====================== 2.20 禁止リスト＠差出人 0 1 H 00000000 From aaa＠xyz.com 00000000 From bbb＠xxx.co.jp 00000000 From ＠ooo.com 00000000 From ＠*adult*.* ====================== こんな感じ。（必要に応じてFrom をReturn-Path やReply-To に置き換え、あるいは追加してやればよい） ポイントは各要素を必須条件とせず、成立要素数を「１」にすること。 （注：この例では便宜上、アットマークを全角で表記しています。実際には小文字で表記してください） リンク ■2chスレ 【SPAM】Spam Mail Killer その1【感染メール除去】 お役立ちリンク NTP - wiki@nothing--補助設定＞NTPサーバーにどこのを入れたらいいのか判らない方のために ※サーバ負荷分散のため、clock.nc.fukuoka-u.ac.jp (133.100.9.2) を使うのは控えましょう！ サルにもわかる正規表現入門--正規表現を理解するために 訪問者 - 本日訪問者 - 昨日訪問者 -
https://w.atwiki.jp/metalmetabo/pages/28.html
- visitors Application of regulatory sequence analysis and metabolic network analysis to the interpretation of gene expression data psu.edu [PDF]J Van Helden, D Gilbert, L Wernisch, M Schroeder, … - Computational …, 2001 - Springer http //scholar.google.com/scholar?q=related JS_4j3CP5nEJ scholar.google.com/ hl=ja as_sdt=2000 Comparative metabolic network analysis of two xylose fermenting recombinant Saccharomyces cerevisiae strains T Grotkjær, P Christakopoulos, J Nielsen, L … - Metabolic Engineering, 2005 - Elsevier http //scholar.google.com/scholar?q=related m6nOh8HQbaIJ scholar.google.com/ hl=ja as_sdt=2000 The recombinant xylose fermenting strain Saccharomyces cerevisiae TMB3001 can grow on xylose, but the xylose utilisation rate is low. One important reason for the inefficient fermentation of xylose to ethanol is believed to be the imbalance of redox co-factors. In the present ... The Edinburgh human metabolic network reconstruction and its functional analysis nih.gov [HTML]H Ma, A Sorokin, A Mazein, A Selkov, E … - Molecular systems …, 2007 - nature.com http //scholar.google.com/scholar?q=related pgWLepUKeS0J scholar.google.com/ hl=ja as_sdt=2000 ... A main objective of human metabolic network analysis is to see how it is related with human disease. More than 10 000 human genes (half of the whole genome) have been reported to be related with one or more human diseases in the OMIM database (Hamosh et al, 2005). ... Reconstruction of metabolic networks from genome data and analysis of their global structure for various organisms oxfordjournals.org [PDF]H Ma, AP Zeng - Bioinformatics, 2003 - Oxford Univ Press http //scholar.google.com/scholar?q=related SuHZgSLeNw8J scholar.google.com/ hl=ja as_sdt=2000 ... It is one of the main tasks of metabolic network analysis to find possible conversion pathways between two metabolites. There may be many different pathways between two metabolites, among them the shortest pathway is of particular interest for network analysis. ... Metabolic network analysis of an adipoyl-7-ADCA-producing strain of Penicillium chrysogenum elucidation of adipate degradation J Thykaer, B Christensen, J Nielsen - Metabolic Engineering, 2002 - Elsevier http //scholar.google.com/scholar?q=related gycTvJYwT9kJ scholar.google.com/ hl=ja as_sdt=2000 An adipoyl-7-ADCA-producing, recombinant strain of Penicillium chrysogenum was characterized by metabolic network analysis, with special focus on the degradation of adipate and determination of the metabolic fluxes. Degradation of the side-chain precursor, adipate, causes an ... Potential metabolic limitations in lysine production by Corynebacterium glutamicum as revealed by metabolic network analysis JA Hollander - Applied Microbiology and Biotechnology, 1994 - Springer http //scholar.google.com/scholar?q=related BwIeARCnafMJ scholar.google.com/ hl=ja as_sdt=2000 Corynebacterium glutamicum and closely related Brevi- bacterium species are the most important organisms used for the industrial fermentative production of ly- sine. Although wild-type strains do not excrete lysine at all, classical mutation programs have resulted in strains ... Metabolic flux and metabolic network analysis of Penicillium chrysogenum using 2D [13C, 1H] COSY NMR measurements and cumulative bondomer simulation WA van Winden, WM van Gulik, D … - Biotechnology and …, 2003 - Wiley Online Library http //scholar.google.com/scholar?q=related Jy6SBrqyeHAJ scholar.google.com/ hl=ja as_sdt=2000 Abstract At present two alternative methods are avail- able for analyzing the fluxes in a metabolic network (1) combining measurements of net conversion rates with a set of metabolite balances including the cofactor bal- ances, or (2) leaving out the cofactor balances and fitting the ... Metabolic‐flux and network analysis in fourteen hemiascomycetous yeasts LM Blank, F Lehmbeck, U Sauer - FEMS yeast research, 2005 - Wiley Online Library http //scholar.google.com/scholar?q=related ILl2ugZn3ZcJ scholar.google.com/ hl=ja as_sdt=2000 ... (5). Oxaloacetate14 mit was estimated using Eq. (6)[16] (6). 2.6Metabolic-network analysis. We used a previously constructed metabolic network [1,24], which consists of the PP pathway, glycolysis, TCA cycle, gluconeogenesis, malic enzyme and by-product formation reactions. ... 13C metabolic flux analysis W Wiechert - Metabolic Engineering, 2001 - Elsevier http //scholar.google.com/scholar?q=related Eat1pl-yJGQJ scholar.google.com/ hl=ja as_sdt=2000 ... 6. B. Christensen and J. Nielsen, Metabolic network analysis, a powerful tool in metabolic engineering. Adv. Biochem. Eng. ... 209–231. 7. B. Christensen and J. Nielsen, Metabolic network analysis of Penicillium chrysogenum using 13 C-labeled glucose. Biotechnol. Bioeng. ... Multiple knockout analysis of genetic robustness in the yeast metabolic network tau.ac.il [PDF]D Deutscher, I Meilijson, M Kupiec, E Ruppin - Nature genetics, 2006 - nature.com http //scholar.google.com/scholar?q=related X5cH6RUwUF0J scholar.google.com/ hl=ja as_sdt=2000 ... | PubMed |; Kitano, H. Biological robustness. Nat. Rev. Genet. 5, 826–837 (2004). | Article | PubMed | ISI | ChemPort |; Papp, B. , Pál, C. Hurst, LD Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast. Nature 429, 661–664 (2004). ...
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- visitors Genome-scale reconstruction of the metabolic network in Staphylococcus aureus N 315 an initial draft to the two-dimensional annotation biomedcentral.com [HTML]SA Becker, BØ Palsson - BMC microbiology, 2005 - biomedcentral.com http //scholar.google.com/scholar?q=related 829ZIciJNnkJ scholar.google.com/ hl=ja as_sdt=2000 ... J Biol Chem 2004, 279 39532-39540. PubMed Abstract | Publisher Full Text OpenURL. Papp B, Pal C, Hurst LD Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast. Nature 2004, 429 661-664. ... Adaptive evolution of bacterial metabolic networks by horizontal gene transfer linkgroup.hu [PDF]C Pál, B Papp, MJ Lercher - Nature Genetics, 2005 - nature.com http //scholar.google.com/scholar?q=related j65eRY_pFyIJ scholar.google.com/ hl=ja as_sdt=2000 ... were present. Gene families with more than one member in E. coli K-12 were excluded from the analysis. Metabolic network analysis. We examined the reconstructed metabolic network (iJR904 GSM/GPR) of E. coli K-12. We ... Network analysis of metabolic enzyme evolution in Escherichia coli biomedcentral.com [HTML]S Light, P Kraulis - BMC bioinformatics, 2004 - biomedcentral.com http //scholar.google.com/scholar?q=related M0gfPqO-WsQJ scholar.google.com/ hl=ja as_sdt=2000 ... representation of metabolic pathways where the substrates and products are the vertices and the enzymes catalyzing the reactions are the edges (Figure 1). The type of network representation used in our study has been used before for metabolic network analysis where it has ... Metabolic network analysis on Phaffia rhodozyma yeast using 13C-labeled glucose and gas chromatography-mass spectrometry C Cannizzaro, B Christensen, J Nielsen, U … - Metabolic Engineering, 2004 - Elsevier http //scholar.google.com/scholar?q=related xVq5KkaMAd4J scholar.google.com/ hl=ja as_sdt=2000 Amino acid labeling patterns, as determined by GC–MS, were in accordance with a metabolic network consisting of the Embden–Meyerhof–Parnas pathway, the pentose phosphate pathway, and the TCA cycle. Glucose was mainly consumed along the pentose ... Metabolic network analysis integrated with transcript verification for sequenced genomes A Manichaikul, L Ghamsari, EFY Hom, C Lin, RR … - Nature methods, 2009 - nature.com http //scholar.google.com/scholar?q=related qlItzvkCLowJ scholar.google.com/ hl=ja as_sdt=2000 Present availability of genome sequences for diverse microorganisms brings opportunities for metabolic engineering through systems-level characterization of these organisms metabolic networks 1 . Such efforts require both functional and structural annotation of metabolic ... High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints E Fischer, N Zamboni, U Sauer - Analytical biochemistry, 2004 - Elsevier http //scholar.google.com/scholar?q=related AGqwTbFzQbYJ scholar.google.com/ hl=ja as_sdt=2000 Influence of metabolic network structure and function on enzyme evolution biomedcentral.com [HTML]D Vitkup, P Kharchenko, A Wagner - Genome biology, 2006 - biomedcentral.com http //scholar.google.com/scholar?q=related tyk--z8xdr0J scholar.google.com/ hl=ja as_sdt=2000 ... Cold Spring Harbor Press, NY; 1982. OpenURL. Papp B, Pal C, Hurst LD Metabolic network analysis of the causes and evolution of the enzyme dispensability in yeast. Nature 2004,429 661-664. PubMed Abstract | Publisher Full Text OpenURL. ... Metabolomics-and proteomics-assisted genome annotation and analysis of the draft metabolic network of Chlamydomonas reinhardtii genetics.org [HTML]P May, S Wienkoop, S Kempa, B Usadel, N … - Genetics, 2008 - Genetics Soc America http //scholar.google.com/scholar?q=related lrqK6GwpxFIJ scholar.google.com/ hl=ja as_sdt=2000 ... Furthermore, the results will contribute to an improvement of metabolic databases. Presently, our methods are limited to one selected large-scale metabolic network analysis technique, the method of network expansion. Further ... Metabolic network analysis during fed-batch cultivation of Corynebacterium glutamicum for pantothenic acid production first quantitative data and analysis of by- … C Chassagnole, A Diano, F Létisse, ND Lindley - Journal of biotechnology, 2003 - Elsevier http //scholar.google.com/scholar?q=related CJC7H27nMvMJ scholar.google.com/ hl=ja as_sdt=2000 A first generation genetically modified strain of Corynebacterium glutamicum has been assessed for its potential to synthesise and accumulate the vitamin pantothenic acid in the medium using fed-batch cultivation technology, with biomass concentration controlled by isoleucine ... Metabolic modeling of microbial strains in silico ru.nl [PDF]MW Covert, CH Schilling, I Famili, JS Edwards, … - Trends in Biochemical …, 2001 - Elsevier http //scholar.google.com/scholar?q=related pjh6sWp3MRMJ scholar.google.com/ hl=ja as_sdt=2000 ... labeling experiments. Adv. Biochem. Eng. Biotechnol. 54, pp. 109–154. Christensen, B. and Nielsen, J., Metabolic network analysis. A powerful tool in metabolic engineering. Adv. Biochem. Eng. Biotechnol. 66, pp. 209–231. The ...
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- visitors Metabolic network analysis of Streptomyces tenebrarius, a Streptomyces species with an active Entner-Doudoroff pathway nih.gov [HTML]I Borodina, C Scholler, A Eliasson, J … - Applied and …, 2005 - Am Soc Microbiol http //scholar.google.com/scholar?q=related vbWz9Ak9HjwJ scholar.google.com/ hl=ja as_sdt=2000 Streptomyces tenebrarius is an industrially important microorganism, producing an antibiotic complex that mainly consists of the aminoglycosides apramycin, tobramycin carbamate, and kanamycin B carbamate. When S. tenebrarius is used for industrial tobramycin ... Genome-scale reconstruction and in silico analysis of the Clostridium acetobutylicum ATCC 824 metabolic network J Lee, H Yun, AM Feist, BØ Palsson, SY Lee - Applied microbiology and …, 2008 - Springer http //scholar.google.com/scholar?q=related 6k93cgR0CzwJ scholar.google.com/ hl=ja as_sdt=2000 Page 1. GENOMICS AND PROTEOMICS Genome-scale reconstruction and in silico analysis of the Clostridium acetobutylicum ATCC 824 metabolic network Joungmin Lee Hongseok Yun Adam M. Feist Bernhard Ø. Palsson Sang Yup Lee ... Metabolic networks in motion 13C-based flux analysis nih.gov [HTML]U Sauer - Molecular systems biology, 2006 - nature.com http //scholar.google.com/scholar?q=related B0vTesydGZwJ scholar.google.com/ hl=ja as_sdt=2000 ... Curr Opin Biotechnol 16 350–355 | Article | PubMed | ISI | ChemPort |; Borodina I, Schöller C, Eliasson A, Nielsen J (2005) Metabolic network analysis of Streptomyces tenebrarius, a Streptomyces species with an active Entner–Doudoroff pathway. ... Combining genomics, metabolome analysis, and biochemical modelling to understand metabolic networks hindawi.com [PDF]O Fiehn - Comparative and Functional Genomics, 2001 - Wiley Online Library http //scholar.google.com/scholar?q=related -r0O-RbpfxsJ scholar.google.com/ hl=ja as_sdt=2000 ... Metabolomics. The obvious next step in metabolic network analysis is to try to determine metabolic snapshots in a broad and comprehensive way. ... 13 Christensen B, Nielsen J. Metabolic network analysis a powerful tool in metabolic engineering. ... A metabolic network analysis NMR experiment design tool with user interface-driven model construction for depth-first search analysis T Zhu, C Phalakornkule, S Ghosh, IE Grossmann, … - Metabolic …, 2003 - Elsevier http //scholar.google.com/scholar?q=related Ov3p-3IVXxsJ scholar.google.com/ hl=ja as_sdt=2000 A Windows program for metabolic engineering analysis and experimental design has been developed. A graphical user interface enables the pictorial, “on-screen” construction of a metabolic network. Once a model is composed, balance equations are automatically generated. ... Genome-scale reconstruction and analysis of the Pseudomonas putida KT2440 metabolic network facilitates applications in biotechnology plos.org [HTML]J Puchałka, MA Oberhardt, M Godinho, A Bielecka, … - PLoS Comput …, 2008 - dx.plos.org PLoS Computational Biology is an open-access. http //scholar.google.com/scholar?q=related V6wktboEeLMJ scholar.google.com/ hl=ja as_sdt=2000 Integrated analysis of regulatory and metabolic networks reveals novel regulatory mechanisms in Saccharomyces cerevisiae cshlp.org [HTML]MJ Herrgård, BS Lee, V Portnoy, BØ … - Genome research, 2006 - genome.cshlp.org http //scholar.google.com/scholar?q=related -IQUN4gFKUgJ scholar.google.com/ hl=ja as_sdt=2000 ... biomass composition). The integration of the metabolic network analysis and the transcriptional regulatory network model described above is accomplished using the regulated flux balance analysis approach (Covert et al. 2001). In ... Validation of a metabolic network for Saccharomyces cerevisiae using mixed substrate studies ulaval.ca [PDF]PA Vanrolleghem, P de Jong-Gubbels, … - Biotechnology …, 1996 - interscience.wiley.com http //scholar.google.com/scholar?q=related jaCs_pUlSXcJ scholar.google.com/ hl=ja as_sdt=2000 Page 1. ARTICLES Validation of a Metabolic Network for Saccharomyces cerevisiae Using Mixed Substrate Studies Peter A. Vanrolleghem,*,† Patricia de Jong-Gubbels,‡ Walter M. van Gulik,§ Jack T. Pronk,‡ Johannes P. van Dijken,‡ and Sef Heijnen† ... Metabolic‐flux analysis of Saccharomyces cerevisiae CEN. PK113‐7D based on mass isotopomer measurements of 13C‐labeled primary metabolites WA Winden, JC Dam, C Ras, RJ Kleijn, JL … - FEMS yeast …, 2005 - Wiley Online Library Skip to Main Content. ... http //scholar.google.com/scholar?q=related fM6Xi9-F_GIJ scholar.google.com/ hl=ja as_sdt=2000 The efficiency of multi-target drugs the network approach might help drug design arxiv.org [PDF]P Csermely, V Agoston, S Pongor - Trends in pharmacological sciences, 2005 - Elsevier http //scholar.google.com/scholar?q=related U0rlpj3tgrYJ scholar.google.com/ hl=ja as_sdt=2000 ... 6111–6121. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (53). 8 B. Papp et al., Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast, Nature 429 (2004), pp. 661–664. Full Text via CrossRef | View Record in Scopus |.
https://w.atwiki.jp/wiki12_0ch/pages/24.html
SPAMや荒らしの対策方法 BBQ(BBX,DSBL)の導入方法 BBQ(BBX,DSBL)導入方法(test060227版) vara.plの串チェックの後(通常は372行目と373行目の間)に my $RADDR = $ENV{ REMOTE_ADDR }; $RADDR =~ /^(\d+)\.(\d+)\.(\d+)\.(\d+)$/; my $query_addr = "$4.$3.$2.$1.niku.2ch.net"; # BBQ用 # my $query_addr = "$4.$3.$2.$1.bbx.2ch.net"; # BBX用 my $addr = join( . , unpack( C* , gethostbyname($query_addr))); if ($addr eq 127.0.0.2 ) { return 997; } と入れるといいよ BBQ用のところを削除かコメントにして、BBXやDSBL用のところのコメントを外せばBBXのチェックも可能 BBQ導入方法(旧バージョン) http //0ch.mine.nu/test/read.cgi/jikken/1046702960/428-430 2バイト文字を含まない投稿を拒否する vara.plのsub NormalizationContents関数の中に以下を追加 # ASCIIのみ if ($text =~ /^[\x00-\x7F]*$/){ return 160; } info/errmsg.cgiに以下を追加 160 広告規制 半角英数字のみの書き込みは出来ません。 海外からのSPAM投稿 管理CGIの掲示板管理で [各種編集]-[規制ユーザの編集] で、\.com、\.netとかを設定する。 (※\.netで規制するとYahooBBユーザー(softbankなんちゃら.bbtec.net)が書けなくなるので注意) 携帯からの荒らし ID強制表示にしてみる 連続投稿規制を使ってみる スレッドタイトルにもNGワードを適用する方法 vara.plの328行目あたりの@checkKeyにsubjectを追加するだけ。 トラックバックスパムをどう防ぐか？ トラックバック機能を殺せばいい 海外ホスト規制を利用したままp2を書き込めるようにする vara.pl「JPホスト以外規制」のところ、 if ($oSET- Equal( BBS_JP_CHECK , checked )){ my $f = 0; my @hosts = qw(.jp .JP p2.2ch.net .bbtec.net .nifty.com); foreach (@hosts){ if(substr($host, -1 * length($_)) eq $_){ $f = 1; last; } } if(!$f){return 207;} } qw( ～ )の中に半角スペース区切りで、 jp以外の国内ISPのホストでめぼしいところを順次追加。 ブランドショップの書き込みがかなりウザイ 対策考え中... info/ngwords.cgi に書いているが収まらず orz エロ出会い業者がウザイ livedoorのスパムちゃんぷるーがちまちまと対応してくれているようです。 携帯がBBQでひっかかるっていわれたので無理やりifでｗｗ # BBQ+スパムちゃんぷるーチェック(※ただし携帯ドメインは除く) if ( $host !~ /\.(? docomo|ezweb|vodafone|jp-[a-z]|softbank|prin)\.ne\.jp$/ ){ my $addr = join( . , reverse( split(/\./, $ENV{ REMOTE_ADDR } ))); my @dnsbls = ( niku.2ch.net , dnsbl.spam-champuru.livedoor.com ); foreach my $dnsbl (@dnsbls) { return ( 997 ) if ( join( . , unpack( C* , gethostbyname("$addr.$dnsbl"))) eq 127.0.0.2 ); } } 一応test060227以降で動作確認済み
https://w.atwiki.jp/metalmetabo/pages/30.html
- visitors Metabolic pathway analysis of enzyme-deficient human red blood cells T ÇakIr, CS Tacer, KÖ Ülgen - Biosystems, 2004 - Elsevier http //scholar.google.com/scholar?q=related 8w1qIgoYqGcJ scholar.google.com/ hl=ja as_sdt=2000 Five enzymopathies (G6PDH, TPI, PGI, DPGM and PGK deficiencies) in the human red blood cells are investigated using a stoichiometric modeling approach, ie, metabolic pathway analysis. Elementary flux modes (EFMs) corresponding to each enzyme deficiency case ... Reconstruction of metabolic networks from genome data and analysis of their global structure for various organisms oxfordjournals.org [PDF]H Ma, AP Zeng - Bioinformatics, 2003 - Oxford Univ Press http //scholar.google.com/scholar?q=related SuHZgSLeNw8J scholar.google.com/ hl=ja as_sdt=2000 Page 1. BIOINFORMATICS Vol. 19 no. 2 2003 Pages 270–277 Reconstruction of metabolic networks from genome data and analysis of their global structure for various organisms Hongwu Ma1, 2 and An-Ping Zeng1,∗ 1GBF ... Elementary mode analysis a useful metabolic pathway analysis tool for characterizing cellular metabolism CT Trinh, A Wlaschin, F Srienc - Applied microbiology and biotechnology, 2009 - Springer http //scholar.google.com/scholar?q=related hkaWpJnxK5QJ scholar.google.com/ hl=ja as_sdt=2000 Abstract Elementary mode analysis is a useful metabolic pathway analysis tool to identify the structure of a metabolic network that links the cellular phenotype to the corresponding genotype. The analysis can decompose the intricate metabolic network comprised of ... Extreme pathway analysis of human red blood cell metabolism nih.gov [PDF]SJ Wiback, BO Palsson - Biophysical Journal, 2002 - Elsevier http //scholar.google.com/scholar?q=related hVaLEfzPgSEJ scholar.google.com/ hl=ja as_sdt=2000 Constraints-based models regulation of gene expression reduces the steady-state solution space MW Covert, BO Palsson - Journal of theoretical Biology, 2003 - Elsevier http //scholar.google.com/scholar?q=related o9VRtN2gAKQJ scholar.google.com/ hl=ja as_sdt=2000 ... Biol. 203, 249, 283. 26. CH SCHILLING, S. SCHUSTER, BO PALSSON and R.HEINRICH, Metabolic pathway analysis basic concepts and scientific applications in the post-genomic era. Biotechnol. Prog. 15 (1999), pp. 296–303. ... The JAK-STAT signaling network in the human B-cell an extreme signaling pathway analysis nih.gov [HTML]JA Papin, BO Palsson - Biophysical Journal, 2004 - Elsevier http //scholar.google.com/scholar?q=related myiNLachV-QJ scholar.google.com/ hl=ja as_sdt=2000 From genome to cellular phenotype—a role for metabolic flux analysis? A Cornish-Bowden, ML Cárdenas - Nature Biotechnology, 2000 - nature.com http //scholar.google.com/scholar?q=related Q1z877WO_uAJ scholar.google.com/ hl=ja as_sdt=2000 ... whether a bypass exists within a complex network. Metabolic pathway analysis aims to identify indispensable enzymes, thus predicting the effects of their deletion or inhibition. Flux analysis should also allow the determination ... On-line metabolic pathway analysis based on metabolic signal flow diagram H Shi, K Shimizu - Biotechnology and bioengineering, 1998 - interscience.wiley.com http //scholar.google.com/scholar?q=related wjDdrq_SGcQJ scholar.google.com/ hl=ja as_sdt=2000 Abstract In this work, an integrated modeling approach based on a metabolic signal flow diagram and cellular energetics was used to model the metabolic pathway analysis for the cultivation of yeast on glucose. This ap- proach enables us to make a clear analysis of the flow ... Metabolic pathway analysis of recombinant Saccharomyces cerevisiae with a galactose-inducible promoter based on a signal flow modeling approach S Jin, K Ye, K Shimizu - Journal of Fermentation and Bioengineering, 1995 - Elsevier http //scholar.google.com/scholar?q=related 0uzMsbsJrXEJ scholar.google.com/ hl=ja as_sdt=2000 The objective of this work was to develop a signal flow diagram-based modeling approach proposed by Endo et al. (1976) to organize the network of complex metabolic reactions occurring in the living cell, employing directed signal flow diagram in which the enzyme reaction ... Energy balance for analysis of complex metabolic networks nih.gov [PDF]DA Beard, S Liang, H Qian - Biophysical journal, 2002 - Elsevier http //scholar.google.com/scholar?q=related wcAvs5fp3FgJ scholar.google.com/ hl=ja as_sdt=2000
https://w.atwiki.jp/sayosayo/pages/281.html
SPAM メーカー：HormelFoods 評価：★☆☆☆☆ 云わずと知れたランチョンミートの缶詰。 ランチョンミートというのは、加工肉のこと。ハムとかソーセージなんかもランチョンミートの一種。 SPAMは豚のひき肉に香辛料と塩を入れてこねたものを缶の中に入れ、加熱したもの。 前々から食べてみたいとは思っていたのだけれど、結構大きく、大きいがゆえにお値段もそれなりに張るので躊躇していました。 美味しければ量は多いほうがいいのですが、不味かった時のことを考えると、中々手が出るものではありません。 そんな中、会社から救援物資としてSPAMを貰ってきたといって持ってきてくれた人が居ました。 その人の同僚はSPAMを加熱せずに食べたそうです。 まぁ、電気もガスとまっているなら、そのままかじるしか有りませんよね。 美味しくなかったそうですが。 ラードはヘットよりは融点が低いとはいえ、口の中で中々溶けるものではないでしょうから当然でしょうね。 おりしも季節は冬ですし。 ま、それはそれとして。 貰ってきたのなら食べなければなりませんね。 あけてみると、ピンク色の巨大な肉塊が詰まっています。 普通の缶詰のように、汁のようなものはありません。 匂いは多分普通ですが、個人的にちょっと苦手なにおいがします。 何の匂いだろう……脂かなぁ。 取り出して、切ってみるとかなりやわらかい。 普通のハムのような肉っぽい感じは余り無くて、魚肉ソーセージのような感じ。 色もそんな感じだし。 弾力が無いので、なんか微妙。 ぺティナイフじゃなくてスライサー(細長い包丁)とかを使ったほうが良かったかな。 微妙に切り辛い。 ま、下手なだけなんだけどさ。 苦手なにおいがしたので、胡椒をたっぷりふりかけ、脂の匂いだと踏んでカリカリに焼き上げてみた。 まぁ、加工肉はカリカリに焼くのが好きなので匂いに関わらずじっくり焼いただろうけど。 ちなみに、ラードがたっぷり入っているので、油を引かなくても自身の脂でカリカリに仕上がります。 かなり塩辛い食品だと聞いていたけれど、東北人の小生はこれに醤油をたらす。 そして、ご飯の上へ。 そして、かぢる。 苦手な匂いは抑え目になっているものの、まだちょっと鼻に付く。 が、我慢できないほどではない。 食感が外側がカリっとしているのはいいけど、中が柔らかいのでなんとも微妙。 味の方は悪くない。 食感はともかく、匂いさえ何とかなれば素直に食べられる味。 沖縄では卵とかと一緒に炒めると聞いたけど、卵とかを使うと違かったのだろうか。 ともかく、SPAMを金を出して買うことはないであろう、というのが小生の評価。 と、云うわけで、星はひとつです。
https://w.atwiki.jp/metalmetabo/pages/26.html
- visitors Combining pathway analysis with flux balance analysis for the comprehensive study of metabolic systems slu.edu [PDF]… JS Edwards, D Letscher, BØ Palsson - Biotechnology and …, 2000 - interscience.wiley.com http //scholar.google.com/scholar?q=related HtFdZ3DFTgMJ scholar.google.com/ hl=ja as_sdt=2000 ... dimensional space. From metabolic pathway analysis, the edges of the high-dimensional flux cone are vectors that correspond to sys- temically defined “extreme pathways” spanning the ca- pabilities of the system. The addition ... Metabolic pathway analysis of yeast strengthens the bridge between transcriptomics and metabolic networks T Çakir, B Kirdar, KÖ Ülgen - Biotechnology and …, 2004 - Wiley Online Library http //scholar.google.com/scholar?q=related cUtrabByB40J scholar.google.com/ hl=ja as_sdt=2000 Abstract Central carbon metabolism of the yeast Saccha- romyces cerevisiae was analyzed using metabolic pathway analysis tools. Elementary flux modes for three substrates (glucose, galactose, and ethanol) were determined using the catabolic reactions ... Metabolic pathway analysis for rational design of L-methionine production by Escherichia coli and Corynebacterium glutamicum JO Krömer, C Wittmann, H Schröder, E Heinzle - Metabolic engineering, 2006 - Elsevier http //scholar.google.com/scholar?q=related KSxlsQoF_oIJ scholar.google.com/ hl=ja as_sdt=2000 Metabolic pathway analysis was carried out to predict the metabolic potential of Corynebacterium glutamicum and Escherichia coli for the production of L-methionine. Based on detailed stoichiometric models for these organisms, this allowed the calculation of the theoretically optimal ... The genome-scale metabolic extreme pathway structure in Haemophilus influenzae shows significant network redundancy JA Papin, ND Price, JS Edwards, BØ … - Journal of theoretical …, 2002 - Elsevier http //scholar.google.com/scholar?q=related KrUkw9kndisJ scholar.google.com/ hl=ja as_sdt=2000 ... 229–248. Abstract | PDF (428 K) | View Record in Scopus | Cited By in Scopus (198). 19. CHSCHILLING, S. SCHUSTER, BO PALSSON and R. HEINRICH, Metabolic pathway analysis basic concepts and scientific applications in the post-genomic era. Biotechnol. Prog. ... Potential drug targets in Mycobacterium tuberculosis through metabolic pathway analysis psu.edu [PDF]S Anishetty, M Pulimi, G Pennathur - Computational Biology and …, 2005 - Elsevier http //scholar.google.com/scholar?q=related hEJbAmAPhwkJ scholar.google.com/ hl=ja as_sdt=2000 The emergence of multidrug resistant varieties of Mycobacterium tuberculosis has led to a search for novel drug targets. We have performed an insilico comparative analysis of metabolic pathways of the host Homo sapiens and the pathogen M. tuberculosis. Enzymes from the ... In silico metabolic pathway analysis and design succinic acid production by metabolically engineered Escherichia coli as an example jsbi.org [PDF]SY Lee, SH Hong, SY Moon - GENOME INFORMATICS SERIES, 2002 - jsbi.org http //scholar.google.com/scholar?q=related 6Ehoy39R1HUJ scholar.google.com/ hl=ja as_sdt=2000 Abstract The intracellular metabolic fluxes can be calculated by metabolic flux analysis, which uses a stoichiometric model for the intracellular reactions along with mass balances around the intra- cellular metabolites. In this study, we have constructed in silico metabolic pathway ... Determination of redundancy and systems properties of the metabolic network of Helicobacter pylori using genome-scale extreme pathway analysis cshlp.org [HTML]ND Price, JA Papin, BØ Palsson - Genome Research, 2002 - genome.cshlp.org http //scholar.google.com/scholar?q=related hyQpOmehsTYJ scholar.google.com/ hl=ja as_sdt=2000 Metabolic pathway analysis in trypanosomes and malaria parasites. nih.gov [PDF]AH Fairlamb - Philosophical Transactions of the Royal Society B …, 2002 - ncbi.nlm.nih.gov http //scholar.google.com/scholar?q=related x44FtcJTlQwJ scholar.google.com/ hl=ja as_sdt=2000 Identification of novel drug targets is required for the development of new classes of drugs to overcome drug resistance and replace less efficacious treatments. In theory, knowledge of the entire genome of a pathogen identifies every potential drug target in any given microbe. ... A general definition of metabolic pathways useful for systematic organization and analysis of complex metabolic networks uni-erlangen.de [PDF]S Schuster, DA Fell, T Dandekar - Nature biotechnology, 2000 - nature.com http //scholar.google.com/scholar?q=related 4GmcRTKIXlwJ scholar.google.com/ hl=ja as_sdt=2000 ... Trends Biotechnol. 17, 53–60(1999). | Article | PubMed | ISI | ChemPort |; Schilling, CH, Schuster, S., Palsson, BO Heinrich R. Metabolic pathway analysis basic concepts and scientific applications in the post-genomic era. Biotechnol. Prog. 15, 296–303 (1999). ...